Changes in the process performance, sludge production and microbial activity in an activated sludge reactor with addition of a metabolic uncoupler under different operating conditions

Ferrer‐Polonio, Eva; Fernández-Navarro, J.; Alonso, J.L.; Amorós-Muñoz, I.; Bes-Piá, A.; Mendoza–Roca, J.A.

doi:10.1016/j.jenvman.2017.08.009

Cited by 18 publications

(8 citation statements)

References 26 publications

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“…Thus, the inhibited proton gradient generated by TCS produced a lower concentration of sludge in SBR-1 but with a metabolically more active biomass. Other studies also reported the correlation between MHEA increase and COD consumption (Ferrer-Polonio et al, 2017) or the total organic carbon removal in the reactor (Kreutz et al, 2016).…”

Section: Atp Cellular Viability and Microbial Hydrolytic Enzymatic Activities (Mhea)mentioning

confidence: 96%

“…The phenomenon of uncoupled metabolism may be carried out under abnormal conditions such as the presence of inhibitory compounds or some heavy metals (Zn, Ni, Cu, Cr), not optimal temperatures, nutrient limitations, oxic-anoxic (or oxic-anaerobic) cycling conditions and chemical uncouplers (Foladori, 2010;Velho et al, 2016). Some of the substances commonly used as chemical uncouplers are DNP (2,4-dinitrophenol) (Ding et al, 2019), TCS (3,3',4',5tetrachlorosalicylanilide) (Ferrer-Polonio et al, 2017), pNP (para-dinitrophenol) (Zuriaga-Agustí et al, 2016) or another reagents based on the phenolic compounds such as mNP (mnitrohenol), oNP (o-nitrophenol) or pCP (p-chlorophenol) (Wang et al, 2017). TCS has been widely adopted as an environmentally-benign uncoupler to reduce yield of activated sludge.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the process performance and microbial community by addition of the metabolic uncoupler 3,3′,4′,5-tetrachlorosalicylanilide in sequencing batch reactors

Ferrer‐Polonio

Fernández-Navarro

Alonso

et al. 2019

Science of The Total Environment

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A complete study about the effects of 3,3',4',5-tetrachlorosalicylanilide (TCS) on organic matter elimination performance, sludge production and on the microbial community of a biological wastewater treatment process has been performed. For this purpose two sequencing batch reactors (SBR) worked in parallel for 43 days with 0.8 mg•L -1 of TCS (SBR-1) and without this metabolic uncoupler (SBR-2). Results indicated that 63.3% of sludge reduction was achieved in SBR-1. However, COD removal efficiency was maintained in similar values in both reactors (89.1% and 92.1% in SBR-1 and SBR-2, respectively). The exhaustive mixed liquor characterization led to know deeply the action mechanism of TCS.In this way, a 69% of adenosine triphosphate (ATP) reduction was observed in SBR-1 in comparison with values measured in SBR-2. On the contrary, an increase in soluble microbial products (SMP) and DNA concentrations occurred as a consequence of TCS addition. Thus, 2 it could be concluded that sludge reduction due to TCS addition was due to both uncoupling effect and cellular lysis. Also, increase in all microbial hydrolytic enzymatic activities measured was observed, which explained the stable performance achieved in SBR-1 despite to the results explained above. It should be highlighted that this uncoupler should not be used in biological treatments that require nitrogen elimination because nitrifying bacteria were affected by its addition (Nitrosomonas and Nitrospira). Finally, the 16S rRNA gene amplicon sequencing informed that an important reduction of bacterial diversity resulted in SBR-1 due to TCS addition.

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Section: Atp Cellular Viability and Microbial Hydrolytic Enzymatic Activities (Mhea)mentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Changes in the process performance and microbial community by addition of the metabolic uncoupler 3,3′,4′,5-tetrachlorosalicylanilide in sequencing batch reactors

Ferrer‐Polonio

Fernández-Navarro

Alonso

et al. 2019

Science of The Total Environment

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show abstract

“…spectrophotometer. More details about the MHEA measurement can be found in Ferrer-Polonio et al (Ferrer-Polonio et al, 2017). The EU values were divided by the MLVSS (g•L -1 ).…”

Section: Ombr Plant and Operating Conditionsmentioning

confidence: 99%

The role of salinity on the changes of the biomass characteristics and on the performance of an OMBR treating tannery wastewater

et al. 2018

Self Cite

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Tannery wastewaters are difficult to treat biologically due to the high salinity and organic matter concentration. Conventional treatments, like sequential batch reactors (SBR) and membrane bioreactors (MBR), have showed settling problems, in the case of SBR, and ultrafiltration (UF) membrane fouling in the case of MBR, slowing their industrial application. In this work, the treatment of tannery wastewater with an osmotic membrane bioreactor (OMBR) is assessed. Forward osmosis (FO) membranes are characterized by a much lower fouling degree than UF membranes. The permeate passes through the membrane pores (practically only water by the high membrane rejection) from the feed solution to the draw solution, which is also an industrial wastewater (ammonia absorption effluent) in this work. Experiments were carried out at laboratory scale with a FO CTA-NW membrane from Hydration Technology Innovations (HTI). Tannery wastewater was treated by means of an OMBR using as DS an actual industrial wastewater mainly consisting of ammonium sulphate. The monitoring of the biological process was carried out with biological indicators like microbial hydrolytic enzymatic activities, dissolved and total adenosine triphosphate (ATP) in the mixed liquor and microbial population. Results indicated a limiting conductivity in the reactor of 35 mS cm (on the 43th operation day), from which process was deteriorated. This process performance diminution was associated by a high decrease of the dehydrogenase activity and a sudden increase of the protease and lipase activities. The increase of the bacterial stress index also described appropriately the process performance. Regarding the relative abundance of bacterial phylotypes, 37 phyla were identified in the biomass. Proteobacteria were the most abundant (varying the relative abundance between 50.29% and 34.78%) during the first 34 days of operation. From this day on, Bacteroidetes were detected in a greater extent varying the relative abundance of this phylum between 27.20% and 40.45%.

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“…Thus, a double-growth limitation strategy consisting in a prolonged substrate limitation after a sufficient long retention under anaerobic condition, allowed obtaining a higher excess sludge minimization. Indeed, it was likely that the internal ATP restoration in the mainstream reactor was lower in scheme B compared to scheme A, because of the lower organic carbon availability in the aerobic reactor compared to the anoxic (Ferrer-Polonio et al, 2017).…”

Section: Performances Of Excess Sludge Reductionmentioning

confidence: 99%

Insights on mechanisms of excess sludge minimization in an oxic-settling-anaerobic process under different operating conditions and plant configurations

et al. 2023

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Changes in the process performance, sludge production and microbial activity in an activated sludge reactor with addition of a metabolic uncoupler under different operating conditions

Cited by 18 publications

References 26 publications

Changes in the process performance and microbial community by addition of the metabolic uncoupler 3,3′,4′,5-tetrachlorosalicylanilide in sequencing batch reactors

Changes in the process performance and microbial community by addition of the metabolic uncoupler 3,3′,4′,5-tetrachlorosalicylanilide in sequencing batch reactors

The role of salinity on the changes of the biomass characteristics and on the performance of an OMBR treating tannery wastewater

Insights on mechanisms of excess sludge minimization in an oxic-settling-anaerobic process under different operating conditions and plant configurations

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