Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Inguglia, Luigi; Chiaramonte, Marco; Arizza, Vincenzo; Turiák, Lilla; Vékey, Károly; Drahos, László; Pitonzo, Rosa; Avellone, Giuseppe; Stefano, Vita Di

doi:10.1371/journal.pone.0228893

Cited by 11 publications

(7 citation statements)

References 62 publications

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“…Aquaculture fish management practices can represent an important stressing factor for fishes, in particular for salmon, and could result in high mortalities leading to significant economic loss for producers (Wilson et al, 2009;Sudheesh et al, 2012;Overton et al, 2019). In fact, stressors like handling, stripping of brood stock, antimicrobial treatments, vaccination, temperature, crowding, starvation and transport can result in an increase of a number of diseases evaluable through the measure of the levels of cytokines, heat shock proteins (HSP), corticosteroid hormones, immunoglobulin and immune cells levels, haematological parameters (Gabriel & Akinrotimi, 2011;Cordero et al, 2016;Rehman et al, 2017;Parisi et al, 2017;Chiaramonte et al, 2019;Cammilleri et al, 2019a;Inguglia et al, 2020;Vazzana et al, 2020). Moreover, salmon, which are usually farmed in crowded conditions, are easily targeted by infective pathologies (Poppe, Barnes & Midtlyng, 2002;Håstein, 2004;Bang-Jensen, Gu & Sindre, 2019).…”

Section: Discussionmentioning

confidence: 99%

Salmo salar fish waste oil: Fatty acids composition and antibacterial activity

Inguglia

Chiaramonte

Stefano

et al. 2020

PeerJ

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Background and aims Fish by-products are generally used to produce fishmeal or fertilizers, with fish oil as a by-product. Despite their importance, fish wastes are still poorly explored and characterized and more studies are needed to reveal their potentiality. The goal of the present study was to qualitatively characterize and investigate the antimicrobial effects of the fish oil extracted from Salmo salar waste samples and to evaluate the potential use of these compounds for treating pathogen infections. Methods Salmo salar waste samples were divided in two groups: heads and soft tissues. Fatty acids composition, and in particular the content in saturated (SAFAs), mono-unsaturated (MUFAs) and Polyunsaturated (PUFAs) fatty acids, was characterized through GC/MS Thermo Focus GC-DSQ II equipped with a ZB-5 fused silica capillary tubes column. The antimicrobial activity of the salmon waste oils was evaluated through the Minimum Inhibitory Concentration assay and the antibiotics contamination was determined by Liquid Chromatography with tandem Mass Spectrometry (LC-MS/MS) analysis. All experiments were done at least in triplicate. Results GC/MS analysis has shown the specific fatty acid composition of the salmon waste oils and their enrichment in MUFAs and PUFAs, with special reference to omega-3, -6, -7, -9 fatty acids. Furthermore, our study has highlighted the antimicrobial activity of the fish waste oil samples against two Gram+ and Gram- bacterial strains. Conclusions These data confirm that the fish waste is still quantitatively and qualitatively an important source of available biological properties that could be extracted and utilized representing an important strategy to counteract infective diseases in the context of the circular economy.

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Section: Discussionmentioning

confidence: 99%

Salmo salar fish waste oil: Fatty acids composition and antibacterial activity

Inguglia

Chiaramonte

Stefano

et al. 2020

PeerJ

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“…The coelomocytes were collected from specimens exposed to NSW (controls) and to those exposed to micro-PS/L and analysed by a label free shotgun proteomic approach for the identification and quantification of all the proteins expressed as previously described by Inguglia et al (2020). In the present study this approach allowed to identify 1323, 1305, 1152, 1275 and 1152 proteins in CTR, 10 micro-PS/L, 50 micro-PS/L, 10 3 micro-PS/L and 10 4 micro-PS/L, respectively, as reported in the corresponding Supplementary Tables S1-S5.…”

Section: Impact Of Micro-ps On the Proteome Of Coelomocytesmentioning

confidence: 99%

Response to microplastic exposure: An exploration into the sea urchin immune cell proteome

Murano

Nonnis

Scalvini

et al. 2023

Environmental Pollution

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“…With the expression of several rSpTrf proteins from insect cells, the hypothesis was tested using rSpTrf proteins bound to inert beads. The use of inert beads removed the confounding effects of surface PAMPS on bacteria, such as LPS, that are detected by sea urchin cells ( 56 – 60 ), induce the expression of the SpTrf gene family ( 30 ), and likely drive phagocytosis of Gram negative bacteria ( 38 ). The use of rSpTrf::beads limited the interactions between the cells and beads exclusively to the phagocyte cell surface and the rSpTrf proteins.…”

Section: Resultsmentioning

confidence: 99%

Recombinant SpTransformer proteins are functionally diverse for binding and phagocytosis by three subtypes of sea urchin phagocytes

Crow,

Shaw,

Grayfer

et al. 2024

Front. Immunol.

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IntroductionThe California purple sea urchin, Strongylocentrotus purpuratus, relies solely on an innate immune system to combat the many pathogens in the marine environment. One aspect of their molecular defenses is the SpTransformer (SpTrf) gene family that is upregulated in response to immune challenge. The gene sequences are highly variable both within and among animals and likely encode thousands of SpTrf isoforms within the sea urchin population. The native SpTrf proteins bind foreign targets and augment phagocytosis of a marine Vibrio. A recombinant (r)SpTrf-E1-Ec protein produced by E. coli also binds Vibrio but does not augment phagocytosis.MethodsTo address the question of whether other rSpTrf isoforms function as opsonins and augment phagocytosis, six rSpTrf proteins were expressed in insect cells. ResultsThe rSpTrf proteins are larger than expected, are glycosylated, and one dimerized irreversibly. Each rSpTrf protein cross-linked to inert magnetic beads (rSpTrf::beads) results in different levels of surface binding and phagocytosis by phagocytes. Initial analysis shows that significantly more rSpTrf::beads associate with cells compared to control BSA::beads. Binding specificity was verified by pre-incubating the rSpTrf::beads with antibodies, which reduces the association with phagocytes. The different rSpTrf::beads show significant differences for cell surface binding and phagocytosis by phagocytes. Furthermore, there are differences among the three distinct types of phagocytes that show specific vs. constitutive binding and phagocytosis. ConclusionThese findings illustrate the complexity and effectiveness of the sea urchin innate immune system driven by the natSpTrf proteins and the phagocyte cell populations that act to neutralize a wide range of foreign pathogens.

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Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity

Cited by 11 publications

References 62 publications

Salmo salar fish waste oil: Fatty acids composition and antibacterial activity

Salmo salar fish waste oil: Fatty acids composition and antibacterial activity

Response to microplastic exposure: An exploration into the sea urchin immune cell proteome

Recombinant SpTransformer proteins are functionally diverse for binding and phagocytosis by three subtypes of sea urchin phagocytes

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