Changes in the rate of food vacuole formation during early clonal life of<i>Paramecium primaurelia</i>

Ramoino, Paola

doi:10.1080/11250009309355803

“…Food vacuole formation in ciliates, including Paramecium, is sensitive to external environmental changes like temperature, pH, size and concentration of particles in the medium. Furthermore, Ramoino [20] described that in Paramecium primaurelia under constant conditions, food vacuole formation rate increased at different life span clones.…”

Section: Food Vacuole Formation In Paramecium Aureliamentioning

confidence: 99%

Uptake of plastic microbeads by ciliate Paramecium aurelia

Nugroho

¹

,

Fyda

²

2020

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Microplastics (MPs) are small fraction of plastics that are less than 5 mm in length. They are bountiful and widespread pollutants in the aquatic environment. A wide range of organisms which play an important role in the food web, ingest microplastic particles and transfer them to the higher trophic levels. In this work, ingestion of fluorescent polystyrene beads 2 µm of diameter by ciliated protozoa Paramecium aurelia in different concentrations and times of exposure was studied. We studied also the ingestion and clearance rate as well as formation of food vacuoles. The highest uptake of beads by ciliates reached 1047.2 ± 414.46 particles after 10 min of incubation. Food vacuoles formation reflected the ingestion rate of P. aurelia, which increased at higher beads concentration up to the10th minute of incubation and decreased afterwards. On the contrary, the clearance rate persisted to be higher at low concentration. These findings showed that maximum capacity of microplastics ingestion by paramecia depended on beads concentration and on time of exposure.

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“…Moreover, studies of phagocytosis in P. primaurelia showed that the food vacuole formation capacity varied during early clonal life [3] and that these changes were related to variations in protein synthesis [4]. It was, however, not explained why protein synthesis decreased despite the fact that the cell took up a large amount of precursors.…”

Section: Introductiollmentioning

confidence: 99%

Image analysis of lysosomal activity during the early clonal life of Paramecium primaurelia

Ramoino

¹

1995

FEMS Microbiology Letters

0

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Acid phosphatase activity was measured in individual cells by determining their optical densities through a scanning confocal laser microscope. The naphthol AS-TR (3-hydroxy-2-naphtoic acid 4'-chloro-2'-methylanilide) phosphate-hexazotized para-rosanilin method was used to visualise the acid phosphatase content in the light microscope. Evidence was obtained that the amount of enzyme varied in exponential growth phase cells as the fission age increased. By comparing the acid phosphatase activity with the rate of food vacuole formation, it appeared that the amount of enzyme inside the cells decreased in early clonal life, whereas the rate of food uptake increased. It was assumed that the reduction of acid phosphatase content could lead to a more extended life of vacuoles and to a decreased membrane recycling rate. In turn, the reduced supply of membrane available for food vacuole formation could partly be responsible for the decrease of the food uptake rate observed after the initial increase.

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Image analysis of lysosomal activity during the early clonal life ofParamecium primaurelia

Ramoino

¹

,

Beltrame

²

,

Fato

³

1995

FEMS Microbiology Letters

3

0

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Acid phosphatase activity was measured in individual cells by determining their optical densities through a scanning confocal laser microscope. The naphthol AS-TR (3-hydroxy-2-naphtoic acid 4'-chloro-2'-methylanilide) phosphate-hexazotized para-rosanilin method was used to visualise the acid phosphatase content in the light microscope. Evidence was obtained that the amount of enzyme varied in exponential growth phase cells as the fission age increased. By comparing the acid phosphatase activity with the rate of food vacuole formation, it appeared that the amount of enzyme inside the cells decreased in early clonal life, whereas the rate of food uptake increased. It was assumed that the reduction of acid phosphatase content could lead to a more extended life of vacuoles and to a decreased membrane recycling rate. In turn, the reduced supply of membrane available for food vacuole formation could partly be responsible for the decrease of the food uptake rate observed after the initial increase.

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Changes in the rate of food vacuole formation during early clonal life ofParamecium primaurelia

Cited by 3 publications

References 19 publications

Uptake of plastic microbeads by ciliate Paramecium aurelia

Uptake of plastic microbeads by ciliate Paramecium aurelia

Image analysis of lysosomal activity during the early clonal life of Paramecium primaurelia

Image analysis of lysosomal activity during the early clonal life ofParamecium primaurelia

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