Changes in the vaginal microbiota associated with primary ovarian failure

Wang, Juan; Xu, Jieying; Han, Qixin; Chu, Weiwei; Lü, Gang; Chan, Wai‐Yee; Qin, Yingying; Du, Yanzhi

doi:10.1186/s12866-020-01918-0

Cited by 18 publications

(18 citation statements)

References 59 publications

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“…Early studies established the paradigm that, following menopause, vaginal Lactobacillus decreases, and overall bacterial levels and diversity are altered ( 22 ). Similar results have been observed in women with POF ( 23 , 24 ). POI, POF, early menopause, and natural menopause reflect different stages of ovarian aging.…”

Section: Introductionsupporting

confidence: 89%

“…Wang et al. found that the relative abundance of Lactobacillus was significantly reduced in POF patients and it is less abundant in postmenopausal women than in POF patients ( 23 ). Wu et al.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, two studies showed that the diversity and richness of the vaginal microbiota of POF patients were statistically different from healthy controls. Wang et al found that the relative abundance of Lactobacillus was significantly reduced in POF patients and it is less abundant in postmenopausal women than in POF patients (23). Wu et al also found a significant decrease in Lactobacillus in POI patients with FSH > 40 IU/ L (24).…”

Section: Interpretation Of the Findingsmentioning

confidence: 99%

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Vaginal Microbiota Changes in Patients With Premature Ovarian Insufficiency and Its Correlation With Ovarian Function

Wen¹,

Feng²,

Yan³

et al. 2022

Front. Endocrinol.

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ObjectivesTo reveal the characteristics of vaginal microbiota in premature ovarian insufficiency (POI) patients and their relationship with ovarian function.Materials and MethodsIn this case-control study, the vaginal bacterial composition of 30 POI patients and 26 healthy women of comparable age was assessed by 16S rRNA gene sequencing targeting the V3-V4 hypervariable regions. The metabolic functions of vaginal microflora were preliminarily predicted through the PICRUSt2 analysis. Redundancy analysis and Spearman’s correlation analyzed the relationships between vaginal microbiota and ovarian function indicators.ResultsActinobacteria, Atopobium, and Gardnerella were significantly increased in POI patients. Their increments were significantly negatively correlated with anti-müllerian hormone (AMH) and inhibin B, and positively correlated with follicle-stimulating hormone (FSH) and luteinizing hormone (LH). While Bifidobacterium was significantly decreased in POI patients. Its relative abundance was significantly positively correlated with AMH and negatively correlated with FSH and LH. Then, POI patients included in this study were divided into POI (25 < FSH ≤ 40) (n = 9) and premature ovarian failure (POF) (FSH > 40) (n = 21) subgroups according to serum FSH levels. Compared with the controls, Firmicutes and Lactobacillus were significantly decreased only in POF (FSH > 40) patients, while no difference was observed in POI (25 < FSH ≤ 40) patients. Lactobacillus was negatively correlated with FSH. Firmicutes was significantly reduced and Actinobacteria was significantly increased in POF (FSH > 40) patients compared with POI (25 < FSH ≤ 40) patients. The key bacterial taxa Gardnerella and Atopobium showed potency in predicting POI.ConclusionsHere we demonstrated significant changes in the vaginal microbiota of POI patients, and these changes were significantly correlated with reduced ovarian reserve, endocrine disruption, and symptoms of perimenopausal syndrome. Differences in vaginal microbiota between POI (25 < FSH ≤ 40) and POF (FSH > 40) patients were also identified. These findings may provide new evidence for the relationship between vaginal microbiota and ovarian function.

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Section: Introductionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Interpretation Of the Findingsmentioning

confidence: 99%

See 1 more Smart Citation

Vaginal Microbiota Changes in Patients With Premature Ovarian Insufficiency and Its Correlation With Ovarian Function

Wen¹,

Feng²,

Yan³

et al. 2022

Front. Endocrinol.

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“…In the control group, the three highest relative expression dominant microbiomes were Lactobacillus, Muribaculaceae and Monoglobus. Lactobacillus plays an important role in maintaining human health and is considered as one of the most important probiotics in human intestinal [31]. It had been reported that Lactobacillus could inhibit the growth and reproduction of pathogenic bacteria [32].…”

Section: Discussionmentioning

confidence: 99%

Effect of Electro-Acupuncture on Gut Microbiome in Cisplatin-Induced Premature Ovarian Failure Mice

He¹,

Guo²,

Zhang³

et al. 2021

Preprint

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Background Growing evidences showed that gut microbiota is associated with premature ovarian failure (POF). Many case reports had shown that electro-acupuncture was effective in the treatment of POF. However, there was little research on regulating gut microbiome of POF mice by electro-acupuncture. Therefore, this study aimed to verify whether electro-acupuncture could improve ovarian function and regulate gut microbiome in mice with POF. In addition, the therapeutic effects of electro-acupuncture at acupoints and non-acupoints were compared. Methods POF mice were established by injected intraperitoneally with cisplatin (2mg/kg) for 2 weeks. Guanyuan (CV4) and Sanyinjiao (SP6) were selected in the electro-acupuncture at acupoints group (EA group). Non-acupoints which around CV4 and SP6 were selected in the electro-acupuncture at non-acupoints group (EN group). EA group and EN group were treated for 3 weeks. The ovarian function was evaluated by assays of histopathological and molecular. Meanwhile, the gut microbiome of POF mice was detected by 16S rDNA sequencing. Results The results showed that EA could restore the estrous cycle and reduce the number of atresia follicles in POF mice. The levels of serum follicle stimulating hormone and luteinizing hormone were decreased by EA. As well as, the levels of serum estradiol, anti mullerian hormone and β-glucuronidase were increased by EA. The relative expressions of PI3K, AKT and mTOR were increased to promote the proliferation of ovarian cells in EA group. According to the results of 16S rDNA sequencing, we found that the diversity of gut microbiome in mice with POF was changed. However, the abundance and diversity of gut microbiome could be regulated by EA in POF mice. The relative abundance of beneficial bacteria was increased by EA. The KEGG pathway analysis showed that the gut microbiome which associated with estrogen signaling pathway, oocyte maturation and PI3K-AKT signaling pathway were regulated by EA. Conclusions EA could provide protection against cisplatin-induced ovarian damage. The abundance and diversity of probiotics could be increased by EA. Moreover, EA might treat POF via regulating the gut microbiome.

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“…In menacme and perimenopause, medium and short Gram-positive bacilli are more frequent, and in the event of dysbiosis, a polymicrobial population predominates. With menopause, there appears to be a reduction in lactobacilli associated with higher serum levels of folliclestimulating hormone (FSH) and lower estrogen levels (9). Intriguingly, more recently, researchers observed that in cases of postmenopausal women complaining of vaginal dryness and irritation, there is a significant frequency in the vagina of BVAB1, which morphologically presented as a curved Gram-negative bacillus, similar to Mobiluncus sp.…”

Section: Vaginal Microbiota and Menopausementioning

confidence: 99%

Postmenopausal Vaginal Microbiome and Microbiota

Oliveira

Lima²,

Brito

et al. 2022

Front. Reprod. Health

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The ovulatory cycle has a significant influence on the microbial composition, according to the action of estrogen and progesterone on the stratified squamous epithelium, due to an increase in epithelial thickness, glycogen deposition, and influence on local immunology. The 16S rRNA gene amplification and pyrosequencing study demonstrated that healthy women have community state types (CST), classified as; type “L,” with a predominance of Lactobacillus crispatus, type II, with a predominance of Lactobacillus gasseri, type III, where Lactobacillus iners predominates, and type V with a predominance of Lactobacillus jensenii. Type IV does not identify lactobacilli but a heterogeneous population of bacteria. There seems to be a relationship between increased vaginal bacterial diversity and poverty of lactobacilli with the complaining of vaginal dryness. With menopause, there appears to be a reduction in lactobacilli associated with higher serum levels of follicle-stimulating hormone (FSH) and lower estrogen levels. The evaluation of Gram-stained vaginal smears in postmenopause women must take into account the clinical-laboratory correlation. We should observe two meanly possibilities, atrophy with few bacterial morphotypes, without inflammatory, infiltrate (atrophy without inflammation), and atrophy with evident inflammatory infiltrate (atrophy with inflammation or atrophic vaginitis). The relationship between the microbiome and postmenopausal vulvovaginal symptoms seems to be related to the bacterial vaginal population. However, more robust studies are needed to confirm this impression.

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Changes in the vaginal microbiota associated with primary ovarian failure

Cited by 18 publications

References 59 publications

Vaginal Microbiota Changes in Patients With Premature Ovarian Insufficiency and Its Correlation With Ovarian Function

Vaginal Microbiota Changes in Patients With Premature Ovarian Insufficiency and Its Correlation With Ovarian Function

Effect of Electro-Acupuncture on Gut Microbiome in Cisplatin-Induced Premature Ovarian Failure Mice

Postmenopausal Vaginal Microbiome and Microbiota

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