Changes in transferrin and hepcidin genes expression in the liver of the fish Pseudosciaena crocea following exposure to cadmium

Chen, J.; Shi, Yuhong; Li, M. Y.

doi:10.1007/s00204-008-0297-7

Cited by 33 publications

(22 citation statements)

References 20 publications

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“…Numerous previous studies have revealed deleterious actions of Cd in diverse systems and models (2-5); however, relatively few studies have attempted to determine the potential biological effects of exposure to chronic sublethal Cd levels. Increasing evidence has suggested that Cd may affect the homeostasis of essential metals, through competition or other unknown mechanisms (34,35). Cd burden was previously shown to stimulate the expression of divalent metal transporter 1 in enterocytes (36).…”

Section: Resultsmentioning

confidence: 99%

Cadmium depletes cellular iron availability through enhancing ferroportin translation via iron responsive element

Sun

Wang

et al. 2014

Molecular Medicine Reports

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Abstract. Cadmium (Cd) is a heavy metal that has detrimental effects on various organs. The widespread contamination of Cd in the environment, crops and food sources poses a severe threat to human health. Acute toxicities of Cd have been extensively investigated; however, the health impact of chronic low-dose exposure to Cd, particularly exposure under non-toxic concentrations, has yet to be elucidated. Furthermore, the toxic threshold of Cd is currently unknown. Ferroportin is the only known iron exporter in vertebrate cells, and it has an essential role in controlling iron egress from cells. To the best of our knowledge, the present study is the first to verify the regulation of ferroportin by Cd. Treatment with low-dose Cd (i.e. at sublethal concentrations, without undermining cell viability) increased the protein expression of ferroportin in macrophages, and this was associated with depleted cellular iron levels. Mechanistic investigations revealed that Cd modulated the ferroportin concentration at the translational level, via the iron responsive element located at the 5'-untranslated region of ferroportin. In conclusion, these data provide evidence for the molecular basis by which Cd alters cellular iron availability through elevating concentrations of ferroportin.

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Section: Resultsmentioning

confidence: 99%

Cadmium depletes cellular iron availability through enhancing ferroportin translation via iron responsive element

Sun

Wang

et al. 2014

Molecular Medicine Reports

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“…Transferrin saturation could function as a signal for iron balance maintenance mechanisms. In a cadmium exposure study, changes in the expression of transferrin genes in the liver of the fish Pseudosciaena crocea were shown subsequent to cadmium exposure 25 . In our subtractive hybridization, three transferrin clones were found, and the mRNA expression levels of transferrin were altered in the group exposed to BaP.…”

Section: Dose-dependent Gene Expression Of Cytochrome P450 1a In Bap mentioning

confidence: 99%

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

Won

Yum

Woo

2011

Toxicol. Environ. Health Sci.

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The construction of a subtractive cDNA library of organisms and the analysis thereof have proven meaningful in the search for clones whose expressions are regulated by exterior changes. The principal objective of this study was to identify the differentially expressed genes of marine medaka fish (Oryzias javanicus) under benzo[a]pyrene exposure conditions. Medaka fish were exposed to 100 μg/L of benzo[a]pyrene for 24 hr and its hepatic RNA was extracted; RNA from the livers of non-exposed medaka fish was also extracted. Both RNAs were employed in the construction of the subtractive cDNA library and the nucleotide sequences of differentially expressed clones were analyzed. Twenty-eight genes of the total differentially expressed clones were considered significant; in particular, the transcription of the cytochrome P450 1A gene was upregulated in a dose-dependent manner under various benzo[a]pyrene concentration exposure conditions. The 28 genes were then divided into 4 categories: (1) information storage and processing, (2) cellular processes and signaling, (3) metabolism, and (4) poorly characterized. The data reported herein provide general information regarding the effects of benzo[a]pyrene contamination on marine organisms, and constitute a primary step in the development of novel biomarkers for marine environmental pollution.

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“…It is synthesized by the hepatic cells and secreted into the blood, and level of transferrin saturation may act as a signal for mechanisms that maintain iron balance. The recent study showed the induction of transferrin mRNA in liver upon exposure to cadmium 33 . Similarly, we found that B[k]F exposure resulted in transcriptional change of transferrin gene compared with non-exposed control group (Table 1).…”

Section: Cell Defense Mechanismmentioning

confidence: 99%

Differential gene expression profiling in B[k]F-exposed marine medaka,Oryzias javanicus by subtractive hybridization

Jeon

Woo

Won

et al. 2010

Toxicol. Environ. Health. Sci.

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Benzo[k]fluoranthene (B[k]F) is one of numerous polycyclic aromatic hydrocarbons (PAHs) which is a persistent environmental contaminant in air and water.Upon B[k]F exposure, differential gene expression profiling was conducted in marine medaka fish (Oryzias javanicus) using subtractive hybridization. As a result, forty two differentially expressed candidate genes were induced in B[k]F-exposed fish as compared to control group, and the genes were associated with general metabolism, signal transduction, cell cycle, immune response, cytoskeleton, development, nucleotide/protein binding and some were non-categorized. These identified gene candidates have great potential to be developed as biomarkers for the identification of effects of B[k]F exposure in the environment. The results obtained in this study will offer insight to the physiological change induced by B[k]F exposure and will help assess the molecular mechanisms of B[k]F toxicity.

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Changes in transferrin and hepcidin genes expression in the liver of the fish Pseudosciaena crocea following exposure to cadmium

Cited by 33 publications

References 20 publications

Cadmium depletes cellular iron availability through enhancing ferroportin translation via iron responsive element

Cadmium depletes cellular iron availability through enhancing ferroportin translation via iron responsive element

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

Differential gene expression profiling in B[k]F-exposed marine medaka,Oryzias javanicus by subtractive hybridization

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