Changes in tumor suppressors and inflammatory responses during hydrogen peroxide-induced senescence in rat fibroblasts

Liu, Rui-Fang; Hu, Lan; Wu, Junnan; Wang, Jing-Xuan; Wang, Xinyu; Liu, Zhiyuan; Zhao, Qida; Li, Wenjing; Song, Xudong; Xiao, Jianhua

doi:10.1080/10715762.2022.2037582

Cited by 4 publications

(2 citation statements)

References 56 publications

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“…Several studies have observed the appearance of senescence and oxidative stress in carcinogenesis models, where both senescence and oxidative stress play significant regulatory roles [ 15 ]. For instance, in rat tail-tip fibroblasts, oxidative stress induced by H2O2 enhanced the senescence and growth of fibroblasts, ultimately promoting the fibroblast carcinogenesis [ 39 ]. It's noteworthy that an unhealthy dietary regimen in daily life can contribute to oxidative stress, thereby fostering age-related carcinogenesis [ 24 ].…”

Section: Oxidative Stress and Senescence In Carcinogenesismentioning

confidence: 99%

Interactions between oxidative stress and senescence in cancer: Mechanisms, therapeutic implications, and future perspectives

Li,

Yu,

et al. 2024

Redox Biology

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Section: Oxidative Stress and Senescence In Carcinogenesismentioning

confidence: 99%

Interactions between oxidative stress and senescence in cancer: Mechanisms, therapeutic implications, and future perspectives

Li,

Yu,

et al. 2024

Redox Biology

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“…The H 2 O 2 -induced HepG2 senescent model was established in a manner previously described [32]. HepG2 was incubated for 24 h in the FBS-free MEM supplemented with different concentrations of H 2 O 2 (from 50 to 1000 µM).…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Auricularia auricula Peptides Nutritional Supplementation Delays H2O2-Induced Senescence of HepG2 Cells by Modulation of MAPK/NF-κB Signaling Pathways

Han,

Li,

Zhao

et al. 2023

Nutrients

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Auricularia auricula is a traditional medicinal and edible mushroom with anti-aging effects. Many studies focused on polysaccharides and melanin. However, the anti-aging effects and mechanism of the nutritional supplementation of Auricularia auricula peptides (AAPs) were not elucidated. In this study, AAPs were prepared by enzymolysis of flavor protease and the protective effects on H2O2-induced senescence of HepG2 cells were explored for the first time. The potential mechanism was also investigated. AAPs were mostly composed of low molecular weights with less than 1000 Da accounting for about 79.17%, and contained comprehensive amino acids nutritionally, including seven essential amino acids, aromatic, acidic, and basic amino acids. AAPs nutritional supplementation could significantly decrease the levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA), and increase the activities of antioxidant enzymes (SOD, CAT, and GSH-Px). In addition, the senescence-associated-β-galactosidase (SA-β-gal) activity was restrained, and the expression levels of senescence-associated secretory phenotype (SASP) (IL-6, IL-8, IL-1β, and CXCL2) were also decreased. Ribonucleic acid sequencing (RNA-Seq) was carried out to screen the differentially expressed genes (DEGs) between different groups. GO and KEGG enrichment analysis showed that the mechanism was related to the MAPK/NF-κB signaling pathways. Quantitative real-time PCR (qRT-PCR) analysis and Western blot were carried out to verify the key genes and proteins in the pathways, respectively. AAPs nutritional supplementation resulted a significant down-regulation in key the genes c-fos and c-jun and up-regulation in DUSP1 of the MAPK signaling pathway, and down-regulation in the key genes CXCL2 and IL-8 of the NF-κB signaling pathway. The results of Western blot demonstrate that AAPs nutritional supplementation could inhibit MAPK/NF-κB pathways by reducing the expression levels of IKK, IκB, P65, and phosphorylation of ERK, thus decreasing the inflammatory reaction and delaying cell senescence. It is the first time that AAPs nutritional supplementation was proved to have protective effects on H2O2-induced oxidative damage in HepG2 cells. These results implicate that dietary AAPs could be used as nutrients to reduce the development or severity of aging.

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Gold nanoclusters stabilized with dopa-containing ligands: Catalyst-indicator integrated probe for tumor cell screening

Li,

Pan,

et al. 2025

Talanta

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Changes in tumor suppressors and inflammatory responses during hydrogen peroxide-induced senescence in rat fibroblasts

Cited by 4 publications

References 56 publications

Interactions between oxidative stress and senescence in cancer: Mechanisms, therapeutic implications, and future perspectives

Interactions between oxidative stress and senescence in cancer: Mechanisms, therapeutic implications, and future perspectives

Auricularia auricula Peptides Nutritional Supplementation Delays H2O2-Induced Senescence of HepG2 Cells by Modulation of MAPK/NF-κB Signaling Pathways

Gold nanoclusters stabilized with dopa-containing ligands: Catalyst-indicator integrated probe for tumor cell screening

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