Changes in working memory induced by lipopolysaccharide administration in mice are associated with metabotropic glutamate receptors 5 and contrast with changes induced by cyclooxygenase-2: Involvement of postsynaptic density protein 95 and down syndrome cell adhesion molecule

Stachowicz, Katarzyna; Pańczyszyn-Trzewik, Patrycja; Sowa-Kućma, Magdalena; Misztak, Paulina

doi:10.1016/j.npep.2023.102347

Cited by 3 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the functional association between the other identified e/sQTLs are not known for OUD, the DSCAM gene is in close proximity and highly correlated with Ets2. DSCAM, a neuronal cell adhesion molecule, regulates cellular connectivity [79] and glutamatergic plasticity associated with learning [80,81]. Glutamate-mediated neuroplasticity occurs following heroin experience, and is considered a driving force behind relapse vulnerability [39].…”

Section: Gwasmentioning

confidence: 99%

“…Glutamate-mediated neuroplasticity occurs following heroin experience, and is considered a driving force behind relapse vulnerability [39]. DSCAM is necessary for tasks requiring memory formation [82,83], including those associated with rewarded behaviors [83], and is upregulated in the hippocampus following a learned memory task [81].…”

Section: Gwasmentioning

confidence: 99%

“…Heroin consumption: Several relevant genes were associated with total consumption of heroin across all training sessions. DSCAM, a neuronal cell adhesion molecule, regulates cellular connectivity [70] and glutamatergic plasticity associated with learning [71,72] . Glutamate-mediated neuroplasticity occurs following heroin experience, and is considered a driving force behind relapse vulnerability [36] .…”

Section: Gwasmentioning

confidence: 99%

“…Glutamate-mediated neuroplasticity occurs following heroin experience, and is considered a driving force behind relapse vulnerability [36] . DSCAM is necessary for tasks requiring memory formation [73,74] , including those associated with rewarded behaviors [74] , and is upregulated in the hippocampus following a learned memory task [72] . Furthermore, GWAS analysis in human showed DSCAM acts as a biomarker for cigarette cessation success [75] , and should be explored for heroin consumption as well.…”

Section: Gwasmentioning

confidence: 99%

See 3 more Smart Citations

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Kuhn,

Cannella,

Chitre

et al. 2024

Preprint

View full text Add to dashboard Cite

The increased prevalence of opioid use disorder (OUD) world-wide has made it imperative to disentangle the biological mechanisms contributing to OUD propensity. OUD shows strong heritability, however genetic variants contributing toward vulnerability remain under explored. In the current study, genome-wide association (GWAS) analysis on over 850 male and female heterogeneous stock rats were performed to identify genes underlying heroin-taking, refraining and seeking behaviors. By using an animal model of OUD, we were able to identify genetic variants associated with distinct behaviors contributing to OUD, an experimental design not easily achieved in human GWAS analysis, and not performed to date for OUD GWAS studies. Our findings show, akin to the human population, several heritable traits related with OUD. We also identified genetic variants associated with heroin consumption, escalation of intake, motivation to obtain heroin, and basal levels of analgesic threshold prior to heroin experience, which was behaviorally unrelated to the heroin behaviors. Several of the genes identified are known biomarkers of other substance use disorders and mediators of neurobiological mechanisms underlying addiction-related behaviors. These findings emphasize the need to assess the role of these genetic variants in OUD, thereby augmenting our understanding of risk factors and neurobiological processes associated with OUD vulnerability.

show abstract

Section: Gwasmentioning

confidence: 99%

Section: Gwasmentioning

confidence: 99%

Section: Gwasmentioning

confidence: 99%

Section: Gwasmentioning

confidence: 99%

See 2 more Smart Citations

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Kuhn,

Cannella,

Chitre

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Kuhn,

Cannella,

Chitre

et al. 2024

Preprint

View full text Add to dashboard Cite

The increased prevalence of opioid use disorder (OUD) makes it imperative to disentangle the biological mechanisms contributing to individual differences in OUD vulnerability. OUD shows strong heritability, however genetic variants contributing toward vulnerability remain poorly defined. We performed a genome-wide association study using over 850 male and female heterogeneous stock (HS) rats to identify genes underlying behaviors associated with OUD such as nociception, as well as heroin-taking, extinction and seeking behaviors. By using an animal model of OUD, we were able to identify genetic variants associated with distinct OUD behaviors while maintaining a uniform environment, an experimental design not easily achieved in humans. Furthermore, we used a novel non-linear network-based clustering approach to characterize rats based on OUD vulnerability to assess genetic variants associated with OUD susceptibility. Our findings confirm the heritability of several OUD-like behaviors, including OUD susceptibility. Additionally, several genetic variants associated with nociceptive threshold prior to heroin experience, heroin consumption, escalation of intake, and motivation to obtain heroin were identified. Tom1, a microglial component, was implicated for nociception. Several genes involved in dopaminergic signaling, neuroplasticity and substance use disorders, including Brwd1, Pcp4, Phb1l2 and Mmp15 were implicated for the heroin traits. Additionally, an OUD vulnerable phenotype was associated with genetic variants for consumption and break point, suggesting a specific genetic contribution for OUD-like traits contributing to vulnerability. Together, these findings identify novel genetic markers related to the susceptibility to OUD-relevant behaviors in HS rats.

show abstract

Study on the Mechanism of Dictyophora duplicata Polysaccharide in Reducing Depression-like Behavior in Mice

Yang,

Chen,

Tang

et al. 2024

Nutrients

View full text Add to dashboard Cite

Background/Objectives: Depression is a prevalent worldwide mental health disorder that inflicts significant harm to individuals and society. Dictyophora duplicata is an edible fungus that contains a variety of nutrients, including polysaccharides. This study aims to investigate the monosaccharide composition and molecular weight of the Dictyophora duplicata polysaccharide (DDP-B1), followed by an exploration of its antidepressant effects in chronic unpredictable mild stress (CUMS) mice. Methods: Dictyophora duplicata was purified using a DEAE-52 column and an S-400 column to obtain DDP-B1. The monosaccharide composition and molecular weight of DDP-B1 were investigated via high-performance gel permeation chromatograph. Six-week-old C57BL/6 male mice were utilized for the CUMS modeling to evaluate the antidepressant efficacy of DDP-B1. Fluoxetine served as the positive control group. The depressive-like behaviors and brain pathology of mice were evaluated. Immunofluorescence (IF) staining, metabolomics analysis, and western blot were employed to further investigate the underlying mechanisms. Results: DDP-B1 significantly alleviated the depression-like behavior of CUMS mice and increased the expression of SYN and PSD-95 in the mice’s brains, which was further validated by western blot. Metabolomics analysis indicated a reduction in serum glutamate in CUMS mice following DDP-B1 treatment. Moreover, DDP-B1 treatment led to an increase in levels of GABAAR, BDNF, p-TrkB and p-p70S6K. Conclusions: DDP-B1 regulated abnormalities in the glutamatergic system, subsequently activated the BDNF-TrkB-mTOR pathway and mitigated the pathological manifestations of CUMS mice. This study validated the potential of DDP-B1 as an antidepressant medication and established a theoretical foundation for the development of fungi with similar properties.

show abstract

Changes in working memory induced by lipopolysaccharide administration in mice are associated with metabotropic glutamate receptors 5 and contrast with changes induced by cyclooxygenase-2: Involvement of postsynaptic density protein 95 and down syndrome cell adhesion molecule

Cited by 3 publications

References 49 publications

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Genome-wide association study reveals multiple loci for nociception and opioid consumption behaviors associated with heroin vulnerability in outbred rats

Study on the Mechanism of Dictyophora duplicata Polysaccharide in Reducing Depression-like Behavior in Mice

Contact Info

Product

Resources

About