Changes in X-ray Sensitivity of HeLa Cells during the Division Cycle

Terasima, Toyozo; Tolmach, L. J.

doi:10.1038/1901210a0

Cited by 416 publications

(103 citation statements)

References 4 publications

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“…HeLa cells were synchronized by two well-known methods. One method was that exponentially growing cells were synchronized at the mitotic phase by the mitotic cell selection technique without the use of any drugs (5), and then cultured again in the culture medium. The other was the thymidine-hydroxyurea method.…”

Section: Materials and Methods Cellsmentioning

confidence: 99%

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: Comparison between cell synchronization methods

et al. 2003

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Background: A novel high-throughput analysis, cell array system, was developed for an extensive study of the expression of genes and/or the degradation of gene products at the cellular level. To exemplify the usefulness of this system, we showed the changes in the expression level of cyclin A and B1 during the cell cycle in a single experiment. Methods: We used the cell array system to chase the changes in cyclin A and B1 expression during the cell cycle in HeLa cells. Cells were synchronized by mitotic selection and thymidine-hydroxyurea methods. Cells were harvested at intervals of 1 h from 0 through 23 h. These 48 cell samples were spotted on a circle of the cell array glass slide. Cyclin A and B1 were immunologically stained with Alexa Fluor 488, and nuclear DNA was stained with propidium iodide. The amount of cyclins and nuclear DNA

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Section: Materials and Methods Cellsmentioning

confidence: 99%

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: Comparison between cell synchronization methods

et al. 2003

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“…Variations in the radiosensitivity of cultured mammalian cells at different phases of the division cycle have been known since the early 1960s (Tersima and Tolmach, 1961Tolmach, , 1963Sinclair and Morton, 1963. It was not, however, until the late 1960s and early 1970s that the high LET radiations were found to diminish the amplitude of the variation between the most sensitive and the most resistant phases.…”

Section: E Cell Age Response and Progression Effectsmentioning

confidence: 99%

Heavy-Ion Radiobiology: Cellular Studies

Blakely

Ngo

Curtis

et al. 1984

Advances in Radiation Biology

199

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“…77,78 When cells were irradiated at different phases of the cell cycle, the cells in M phase showed the greatest sensitivity. Cells at the transition between G 1 and S are also quite radiosensitive, but less than M-phase cells.…”

Section: Effect Of Hormonal Pretreatment On the Effectiveness Of Cytomentioning

confidence: 99%

Cell Cycle Arrest by Glucocorticoids May Protect Normal Tissue and Solid Tumors from Cancer Therapy

Mattern¹,

Büchler²,

Herr³

2007

Cancer Biology & Therapy

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ABBrevIAtIons DEX ABstrActGlucocorticoids have been widely used as cotreatment for patients with cancer due to potent pro-apoptotic properties in lymphoid cells, reduction of nausea and diminishing acute toxicity on normal tissue. There are now data from preclinical and, to some extent, clinical studies, demonstrating that these medicaments are highly suspicious to induce therapy resistance in the majority of malignant solid tumors-irrespective of tumor origin and the nature of specific anticancer drugs or irradiation used for treatment. Despite these huge amounts of data, the underlying mechanisms of cell type-specific signaling by these steroid hormones are just beginning to be described. This review summarizes our present understanding of a relationship between glucocorticoid-induced reversible cell cycle arrest and therapy resistance in solid tumors. We give a summary of our current knowledge of decreased proliferation rates in response to glucocorticoid pre and combination treatment which are suspicious to be involved not only in protection of normal tissues, but also in protection of solid tumors from cytotoxic effects of anticancer agents. The inhibition of cell cycle progression by pretreatment with GCs may be crucially involved in switching the balance of several interacting pathways to survival upon treatment with GCs.

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Changes in X-ray Sensitivity of HeLa Cells during the Division Cycle

Cited by 416 publications

References 4 publications

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: Comparison between cell synchronization methods

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: Comparison between cell synchronization methods

Heavy-Ion Radiobiology: Cellular Studies

Cell Cycle Arrest by Glucocorticoids May Protect Normal Tissue and Solid Tumors from Cancer Therapy

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