Changes induced by corticosterone and adrenalectomy in synaptosomal and platelet uptake and binding of 5-HT The relationship to [3H]imipramine binding

Lee, P. H. K.; Chan, Mo-Yin

doi:10.1016/0028-3908(85)90189-3

Cited by 10 publications

(3 citation statements)

References 23 publications

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“…First, glucocorticoids including corticosterone can elevate tryp- tophan levels, and stimulate the uptake of L-[3H]-tryptophan by brain synaptosomes (23,24).…”

Section: Discussionmentioning

confidence: 99%

Blockade of Corticosterone Synthesis Reduces Serotonin Turnover in the Dorsal Hippocampus of the Rat as Measured by Microdialysis

Korte-Bouws

Korte

Kloet³

et al. 1996

J Neuroendocrinology

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The influence of plasma corticosterone concentration on serotonin (5-HT) turnover in the dorsal hippocampus was investigated. The experiments were performed in freely moving male Wistar rats in their home cage. Blood samples were taken via a permanent jugular vein catheter to determine plasma corticosterone levels. Extracellular levels of 5-HT and its metabolite 5-hydroxy-indole acetic acid (5-HIAA) were measured using in vivo microdialysis. The rats received an intravenous (i.v.) infusion of the steroid synthesis-inhibitor metyrapone (150 mg/kg/ml) in order to manipulate circulating corticosterone levels. Three hours later, the monoamine oxidase inhibitor pargyline (15 mg/kg/2 ml i.v.) was administered to produce an accumulation of extracellular 5-HT. Pargyline administration led to a four fold increase in 5-HT levels, while reducing 5-HIAA by 45%. Metyrapone pretreatment blocked the pargyline-induced rise in plasma corticosterone to baseline levels and diminished the pargyline-induced increase in 5-HT, without affecting 5-HIAA levels. Thus, the data suggest that a decrease in availability of corticosterone for its receptors by metyrapone diminished the 5-HT synthesis rate. Since plasma corticosterone levels during this blockade are still low, it is assumed that brain glucocorticoid receptor occupation is reduced, while mineralocorticoid receptors are still substantially occupied. Therefore the present results support the hypothesis that corticosterone through glucocorticoid receptor activation enhances 5-HT synthesis rate and release in the dorsal hippocampus.

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“…First, glucocorticoids including corticosterone can elevate tryp- tophan levels, and stimulate the uptake of L-[3H]-tryptophan by brain synaptosomes (23,24).…”

Section: Discussionmentioning

confidence: 99%

Blockade of Corticosterone Synthesis Reduces Serotonin Turnover in the Dorsal Hippocampus of the Rat as Measured by Microdialysis

Korte-Bouws

Korte

Kloet³

et al. 1996

J Neuroendocrinology

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“…Moreover, future studies should be designed to demonstrate the direct evidence that increased methylation of the 5-HTT (SLC6A4) gene in the PFC is related to a lower binding of the radiotracer DASB to the 5-HTT. 67 , 68 , 69 , 70 , 71 This could be performed by using human autopsy material to gain additionally further mechanistic insights (that is, by the analysis of 5-HTT mRNA levels in different brain tissues) to confirm the accordant in vivo findings. 29 …”

Section: Limitationsmentioning

confidence: 99%

Serotonin transporter gene promoter methylation status correlates with in vivo prefrontal 5-HTT availability and reward function in human obesity

et al. 2017

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A polymorphism in the promoter region of the human serotonin transporter (5-HTT)-coding SLC6A4 gene (5-HTTLPR) has been implicated in moderating susceptibility to stress-related psychopathology and to possess regulatory functions on human in vivo 5-HTT availability. However, data on a direct relation between 5-HTTLPR and in vivo 5-HTT availability have been inconsistent. Additional factors such as epigenetic modifications of 5-HTTLPR might contribute to this association. This is of particular interest in the context of obesity, as an association with 5-HTTLPR hypermethylation has previously been reported. Here, we tested the hypothesis that methylation rates of 14 cytosine–phosphate–guanine (CpG) 5-HTTLPR loci, in vivo central 5-HTT availability as measured with [11C]DASB positron emission tomography (PET) and body mass index (BMI) are related in a group of 30 obese (age: 36±10 years, BMI>35 kg/m2) and 14 normal-weight controls (age 36±7 years, BMI<25 kg/m2). No significant association between 5-HTTLPR methylation and BMI overall was found. However, site-specific elevations in 5-HTTLPR methylation rates were significantly associated with lower 5-HTT availability in regions of the prefrontal cortex (PFC) specifically within the obese group when analyzed in isolation. This association was independent of functional 5-HTTLPR allelic variation. In addition, negative correlative data showed that CpG10-associated 5-HTT availability determines levels of reward sensitivity in obesity. Together, our findings suggest that epigenetic mechanisms rather than 5-HTTLPR alone influence in vivo 5-HTT availability, predominantly in regions having a critical role in reward processing, and this might have an impact on the progression of the obese phenotype.

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“…Regarding the long-term regulation of 5HTt activity (and, consequently, 5HT content) produced for e.g. by endocrine status or chronic in vivo drug administration, there are studies indicating the parallelism in changes in brains and platelets (Bianchi et al, 2002;Lee and Chan, 1985;Jensen et al, 1994), but significant dissimilarities between them have also been reported (Alvarez et al, 1999;Jensen et al, 1994).…”

Section: Introductionmentioning

confidence: 96%

Serotonin transporter kinetics in rats selected for extreme values of platelet serotonin level

Čičin‐Šain¹,

Froebe²,

Bordukalo-Niksic³

et al. 2005

Life Sciences

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Changes induced by corticosterone and adrenalectomy in synaptosomal and platelet uptake and binding of 5-HT The relationship to [3H]imipramine binding

Cited by 10 publications

References 23 publications

Blockade of Corticosterone Synthesis Reduces Serotonin Turnover in the Dorsal Hippocampus of the Rat as Measured by Microdialysis

Blockade of Corticosterone Synthesis Reduces Serotonin Turnover in the Dorsal Hippocampus of the Rat as Measured by Microdialysis

Serotonin transporter gene promoter methylation status correlates with in vivo prefrontal 5-HTT availability and reward function in human obesity

Serotonin transporter kinetics in rats selected for extreme values of platelet serotonin level

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