Changes of acute-phase proteins during different physiological conditions in dairy Saanen goats

Samimi, Amir Saeed; Aghamiri, Seyed Morteza; Nazifi, Saeed; Asadi, Zahra; Farhang, Mona

doi:10.1007/s00580-020-03123-x

Cited by 6 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, CRP concentrations are reduced due to the endogenous release of E2 during the follicular phase [ 35 ], suggesting that estrogens regulate anti-inflammatory effects, reducing the expression of cytokines and molecular adhesion [ 36 ]. Despite this, in non-synchronized estrous cycles in cows, haptoglobin (Hp) was significantly higher compared to diestrus and proestrus [ 37 ]. It seems that behavioral stress during ovulation in buffaloes, as increased oxidative activity and free radical release during estrus, could be other reasons for the increase of Hp [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares

Satué

Fazio

Fauci

et al. 2023

Animals

View full text Add to dashboard Cite

Several studies have demonstrated that in woman the sex hormones such as estrogen (E2) and progesterone (P4) influence iron (Fe) regulation, contributing to variations in Fe parameters along the menstrual cycle. These mechanisms based on the regulation of hepcidin (Hepc) which limits Fe availability during the cycle, remain poorly characterized in healthy mares. The objective of this study was to establish the relationship between Hepc, Fe, ferritin (Ferr), and the primary ovarian hormones E2 and P4 in cycling Purebred Spanish mares. Blood samples were taken from 31 Purebred Spanish mares day −5, on day 0, day +5 and day +16 of the cycle. Fe and Ferr significantly increased and Hepc decreased during pre- and ovulatory periods. The secretion peak of estradiol-17β (E2) was reached on day 0 and progesterone (P4) between days +5 and +16. Fe and Ferr were positively correlated (r = 0.57). Fe and Ferr were negatively correlated with Hepc (r = −0.72 and r = −0.02, respectively). E2 and P4 were negatively and positively correlated with Hepc (r = −0.753 and r = 0.54, respectively). In cycling Purebred Spanish mares there is a measurable relationship between steroid hormones and systemic Fe metabolism. Estrogenic dominance in the pre- and ovulatory period allows for a more effective iron status, mediated by hepcidin inhibition. However, P4 during the luteal phase substantially reduces serum Fe and iron stores, possibly related to Hepc stimulation. Future research is required to clarify the relationship between steroid hormones and iron metabolism at the molecular level in equids.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares

Satué

Fazio

Fauci

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Zhao et al [ 6 ] screened that PON1, GLYCAM1, HP, SCGB1D were differentially expressed in the blood of dairy cows between inactive ovary and the normal estrus. The concentration of HP in blood at estrus phase was significantly higher than diestrus and proestrus in dairy cows [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Differences in Milk Proteomic Profiles between Estrous and Non-Estrous Dairy Cows

Du,

Nan,

et al. 2023

Animals

View full text Add to dashboard Cite

Efficient reproductive management of dairy cows depends primarily upon accurate estrus identification. However, the currently available estrus detection methods, such as visual observation, are poor. Hence, there is an urgent need to discover novel biomarkers in non-invasive bodily fluids such as milk to reliably detect estrus status. Proteomics is an emerging and promising tool to identify biomarkers. In this study, the proteomics approach was performed on milk sampled from estrus and non-estrus dairy cows to identify potential biomarkers of estrus. Dairy cows were synchronized and timed for artificial insemination, and the cows with insemination leading to conception were considered to be in estrus at the day of insemination (day 0). Milk samples of day 0 (estrus group) and day −3 (non-estrus group) from dairy cows confirming to be pregnant were collected for proteomic analysis using the tandem mass tags (TMT) proteomics approach. A total of 89 differentially expressed proteins were identified, of which 33 were upregulated and 56 were downregulated in the estrus milk compared with the non-estrus milk. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that acetyl coenzyme A carboxylase α (ACACA), apolipoprotein B (APOB), NAD(P)H steroid dehydrogenase-like (NSDHL), perilipin 2 (PLIN2), and paraoxonase 1 (PON1) participated in lipid binding, lipid storage, lipid localization, and lipid metabolic process, as well as fatty acid binding, fatty acid biosynthesis, and fatty acid metabolism, and these processes are well documented to be related to estrus regulation. These milk proteins are proposed as possible biomarkers of estrus in dairy cows. Further validation studies are required in a large population to determine their potential as estrus biomarkers.

show abstract

“…However, the application was limited due to the lack of theoretical basis and data support in small ruminants. Samimi et al reported the changes in APPs in three different physiological times [6]. However, the information on the changes in APPs during the periparturient period is still insufficient.…”

Section: Introductionmentioning

confidence: 99%

Changes in Acute-Phase Proteins in Plasma during the Periparturient Period of Dairy Goats

Zeng

Shen

Yuan

et al. 2021

Veterinary Sciences

View full text Add to dashboard Cite

The present study was conducted regarding four acute-phase proteins (APPs) including C-reactive protein (CRP), ceruloplasmin (CP), serum amyloid A (SAA), and haptoglobin (HP) in dairy goats during the periparturient period. The aim of this study was to detect the changes in APPs in plasma during the periparturient period of healthy dairy goats. Guanzhong dairy goats with no other symptoms (n = 15) were selected on the basis of their blood calcium (Ca) and β-hydroxybutyrate (BHBA) concentration. The plasma was collected once a week for ±3 weeks delivery. The concentrations of the four APPs mentioned above were determined using goat-specific ELISA kits. The results showed the CRP level in plasma decreased from 3 weeks to 1 week antepartum and increased later until 1 week postpartum and then decreased to a similar level with antepartum between 1 and 3 weeks postpartum. The content of CP showed a decline in 3 weeks before parturition and an upward trend between 1 week antepartum and 3 weeks postpartum. The SAA concentration decreased from 3 weeks antepartum to 2 weeks postpartum and rebounded later. The level of HP decreased during 3 weeks before parturition and increased until 1 week postpartum, then reached a stable value. Clear variation range and rules of APPs contribute to perinatal health monitoring of dairy goats.

show abstract

Changes of acute-phase proteins during different physiological conditions in dairy Saanen goats

Cited by 6 publications

References 14 publications

Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares

Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares

Differences in Milk Proteomic Profiles between Estrous and Non-Estrous Dairy Cows

Changes in Acute-Phase Proteins in Plasma during the Periparturient Period of Dairy Goats

Contact Info

Product

Resources

About