Changes of aminoacyl‐tRNA synthesis in the liver of rats administered cholesteryl 14‐methylhexadecanoate

Komárková, E.; Hradec, J

doi:10.1016/0014-5793(71)80118-7

Cited by 2 publications

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“…However, significant changes were found in mixtures containing at least supernatant enzymic factors from experimental animals. It seems that cholesteryl 14-methylhexadecanoate interacts primarily with components of the supernatant fraction in a way similar to pH5 enzymes (Kom6rkova & Hradec, 1971a).…”

Section: Discussionmentioning

confidence: 99%

“…Cholesteryl 14-methylhexadecanoate affects the activity of some enzymes required for protein synthesis in rat liver and evidence has been obtained that this ester is present in molecules of aminoacyl-tRNA synthetases (Hradec & Dulek, 1969), and of peptideelongation factors (Hradec et al, 1971), as a normal constituent essential for the function ofthese enzymes. Significant changes of RNA polymerase activity in the nuclei ofliver cells (Komirkovi & Hradec, 1971b), as well as of L-leucyl-tRNA synthetase activity (Komarkov&a & Hradec, 1971a) follow the injection ofthis compound into rats. For a better understanding of the mode of action of this ester, which seems to be an important cofactor in protein synthesis, further studies are required on its possible effect on different enzymic systems, in particular at the level of the expression of the genetic message on the ribosome.…”

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confidence: 99%

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Protein synthesis in the liver of rats injected with cholesteryl 14-methylhexadecanoate

Komárková¹,

Hradec²

1972

Biochemical Journal

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1. Rats were injected intraperitoneally with cholesteryl 14-methylhexadecanoate and killed after various intervals of time up to 3 days; ribosomes and cell sap were isolated from their liver tissue. These fractions were tested for their ability to participate in protein synthesis. 2. Protein synthesis in complete systems containing ribosomes, cell sap and all necessary cofactors was significantly enhanced at 12 and 72h after the injection and significantly inhibited at 24h. At early times after injection isolated ribosomes had a slightly enhanced ability to bind nRNA. Peptide-elongation processes (i.e. binding of aminoacyl-tRNA to ribosomes, peptidyl transfer and polyphenylalanine synthesis) showed significant stimulation or inhibition depending on the time after injection of the ester. 3. A correlation was found between the ability of cell sap to stimulate polyphenylalanine synthesis and the relative cholesteryl 14-methylhexadecanoate content in the postmicrosomal supernatant at different time-intervals after administration of the ester. No significant changes were found in its content in the whole liver tissue. 4. Since the injected ester has previously been shown to accumulate in some enzymic fractions, the changes in its relative content may represent a regulatory mechanism modulating the rate of protein synthesis.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%