Changes of aqueous humor cytokine profiles of patients with high intraocular pressure after PPV for retinal detachment

Abstract

High ocular pressure is one of the early complications after pars plana vitrectomy (PPV), which may cause glaucoma and poor visual prognosis secondary to surgery. It’s essential for the basic mechanism to analyze the relative humor cytokine profiles and clinical factors with intraocular pressure (IOP) after PPV for retinal detachment (RD). In this study, we have collected the aqueous humor of 16 patients with high IOP after PPV and 16 patients with normal IOP after PPV. We qualified 27 cytokines using Luminex multiplex cytokines analysis and compared biomarkers with high IOP group and normal group. In a result, the concentration of VEGF, IL-6, FGF2, and G-CSF upregulated significantly (P < 0.05), while VEGFR2 downregulated significantly (P < 0.05) in high IOP group. IL-6 was positively correlated with high IOP (r = 0.561, P = 0.041). Meanwhile, the concentration of IL-6 (r = 0.543, P = 0.03), IL-5 (r = 0.576, P = 0.019), IL-15 (r = 0.614, P = 0.011), IL-4 (r = 0.517, P = 0.04), ICAM-1 (r = 0.611, P = 0.012) and G-CSF (r = 0.636, P = 0.008) were significantly associated with preoperative PVR classification, and the aqueous humor levels of IL-4 (r = 0.567, P = 0.022), HGF (r = 0.701, P = 0.005), and MCP-1 (r = 0.565, P = 0.035) are significant relative to laser points. Hence, cytokines might potentially be the therapeutic target of high IOP after PPV.