Changes of buoyant density during the S-phase of the cell cycle. Direct evidence demonstrated in acute myeloid leukemia by flowcytometry

Daenen, Simon; Huiges, W; Modderman, E.; Halie, M. R.

doi:10.1016/0145-2126(93)90139-c

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…For example, differences in the buoyant density of animal cells correlate with cell morphology, differentiation status, and phase of the cell cycle (e.g., Daenen et al 1993, Parent et al 1997, Maric et al 1998. And in plants, the buoyant density of protoplasts varies with cell type (Fitzsimons and Weyers 1983).…”

Section: Introductionmentioning

confidence: 99%

The density of apical cells of dark-grown protonemata of the mossCeratodon purpureus

Schwuchow¹,

Kern²,

Wagner³

et al. 2000

Protoplasma

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Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First, the volume fraction of each cell component was determined by stereology, and published values for component density were used to extrapolate to the entire cell. Second, protonemal tips were immersed in bovine serum albumin solutions of different densities, and then the equilibrium density was corrected for the mass of the cell wall. Third, apical cell protoplasts were centrifuged in low-osmolarity gradients, and values were corrected for shrinkage during protoplast isolation. Values from centrifugation (1.004 to 1.015 g/cm3) were considerably lower than from other methods (1.046 to 1.085 g/cm3). This work appears to provide the first corrected estimates of the density of any plant cell. It also documents a method for the isolation of protoplasts specifically from apical cells of protonemal filaments.

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