Changes of Chemical Properties and Microbiological Succession of Lignites with Weathering.

Abstract

Changes of chemical properties of lignite, collected from Shenyang Coal Mine, Liao Ning Province, Northeast China, and microbial succession on it with 4 years of weathering in open air were studied. With weathering, the content of total carbon and nitrogen, and C/N ratio decreased, while the ash content and the amount of organic matter which was extracted with 0.1M NaOH increased distinctly. UV-VIS spectra of humic and fulvic acids obtained from lignite samples changed systematically with weathering. Distribution of fulvic acids into adsorbed and nonadsorbed fractions with a cross-linked polyvinylpyrrolidone (PVP), which were regarded as humic and non-humic substances, respectively, was also changed. However, the IR spectra scarcely differed among the humic acids in lignite samples which were recently excavated and those which were weathered. Microbial succession on lignite was observed with weathering; actinomycetes as the first colonizers, followed by bacteria and then fungi. The dominant microorganisms were Actinosynnema sp., Streptomyces sp. and Nocardia sp. among actinomycetes, two Arthrobacter spp. among bacteria and two Aspergillus spp. among fungi.

A potent endophytic fungus Purpureocillium lilacinumYZ1 protects against Fusarium infection in field‐grown wheat

“…One liter of Martins media for culturing Purpureocillium lilacinum L. YZ1 contained 10 g glucose, 5 g peptone, 1 g KH 2 PO 4 , 0.5 MgSO 4 ·7H 2 O, and 15 g Agar for solid media (Yuan et al ., 1994). For culturing Fusarium graminearum S. ( Fg ), potato dextrose agar (PDA) was prepared by dissolving 46 g of Coolaber Potato Dextrose Agar (Cat no.…”