Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Wang, Zhengchao; Wei, Quanwei; Jiang, Yongqing; Lu, Lizhi; Wang, Hao; Watanabe, Gen; Taya, Kazuyoshi

doi:10.1262/jrd.18156

Cited by 17 publications

(19 citation statements)

References 44 publications

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“…In agreement with the present results, treatment of immature rats with eCG increased both cAMP levels and PDE activities in ovarian homogenates (Wang et al 2007), and gonadotropin stimulation caused the activation of PDE via an increase in cAMP in cultured Sertoli (Conti et al 1982) and granulosa cells (Conti et al 1984). Further studies demonstrated that TSH quickly activated the cAMP-specific PDE in the thyroid cells through a phosphorylation mediated by the catalytic subunit of PRKA (Sette et al 1994).…”

Section: Puberty and Oocyte Meiotic Resumptionsupporting

confidence: 91%

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Han¹,

Cao²,

Wang³

et al. 2010

Reproduction

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Although studies suggest that the low competence of oocytes from prepubertal animals is due to their insufficient cytoplasmic maturation and that FSH improves oocyte maturation possibly by retarding meiotic progression and allowing more time for cytoplasmic maturation, the mechanisms by which puberty and gonadotropins regulate meiotic progression require additional detailed studies. For the first time, we observed that while meiotic progression was significantly slower, the maturation-promoting factor (MPF) activity of oocytes was significantly higher in prepubertal than in adult mice. To resolve this contradiction, we specified the molecules regulating the MPF activity and their localization during oocyte maturation in prepubertal and adult mice primed with or without gonadotropins. Our tests using corresponding enzyme regulators suggested that while activities of protein kinase A were unaffected, the activity of adenylate cyclase (ADCY) and phosphodiesterase increased while cell division cycle 2 homolog A (CDC2A) decreased significantly after puberty. While most of the adult oocytes had CDC2A protein concentrated in the germinal vesicle (GV) region, the majority of prepubertal oocytes showed no nuclear concentration of CDC2A. Maximally priming mice with equine chorionic gonadotropin brought the above parameters of prepubertal oocytes close to those in adult oocytes. Together, the results suggest that puberty and gonadotropin control oocyte meiotic progression mainly by regulating the ADCY activity and the concentration of the activated MPF toward the GV region.

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Section: Puberty and Oocyte Meiotic Resumptionsupporting

confidence: 91%

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Han¹,

Cao²,

Wang³

et al. 2010

Reproduction

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“…Our morphological examination showed that typical histological changes were consistent with those observed in our previous report (Wang et al, 2007). At postnatal day 1, the ovary contained only primordial follicles with an oocyte partially or completely encapsulated by squamous pre-granulosa cells ( Figure 1A), and HIF-1α immunoreactivities were markedly localized to the oocytes ( Figure 1B).…”

Section: Follicular Development and Hif-1α Immunohistochemistry In Thsupporting

confidence: 89%

“…1) Postnatal rat model (Wang et al, 2007): neonatal rats were obtained by mating adult Sprague-Dawley males and females. After birth, neonatal rats at 1, 5, 7, 10, and 21 days of age (the day of birth was considered day 1) were sampled under ether anesthesia, and the ovaries were immediately collected.…”

Section: Experimental Designmentioning

confidence: 99%

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Expression of hypoxia-inducible factor-1α during ovarian follicular growth and development in Sprague-Dawley rats

et al. 2015

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ABSTRACT. Hypoxia-inducible factor-1α (HIF-1α) has been identified as a transcription factor that is involved in diverse physiological and pathological processes in the ovary. In this study, we examined whether HIF-1α is expressed in a cell-and stage-specific manner during follicular growth and development in the mammalian ovaries. Using immunohistochemistry and Western blot analysis, HIF-1α expression was observed in granulosa cells specifically and was significantly increased during the follicular growth and development of postnatal rats. Furthermore, pregnant mare serum gonadotropin also induced HIF-1α expression in granulosa cells and ovaries during the follicular development of immature rats primed with gonadotropin. Moreover, we also examined proliferation cell nuclear antigen, a cell proliferation marker, during follicular growth and development and found that its expression pattern was similar to that of HIF-1α protein. Granulosa cell culture experiments revealed that proliferation cell nuclear antigen expression may be regulated by HIF-1α. These results indicated that HIF-1α plays an important role in the follicular growth and development of these 2 rat models. The HIF-1α-mediated signaling pathway may be an important mechanism regulating follicular growth and development in mammalian ovaries in vivo.

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“…The amount of bound radioactivity was determined by γ counting. The caMP level of each sample was measured using commercial ria kits as previously described (12).…”

Section: Methodsmentioning

confidence: 99%

Fibronectin increases RhoA activity through inhibition of PKA in the human gastric cancer cell line SGC-7901

Chen

Yan

et al. 2010

Mol Med Rep

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Abstract. Fibronectin/integrin-mediated signaling plays a key role in the regulation of adhesion, migration and metastasis of tumors. Numerous studies have addressed the significance of the association between integrin and rhoa, but the exact mechanism is unclear. results from laboratories, including ours, have demonstrated that PKa inhibits the activity and function of rhoa. This study was designed to investigate the relationships among the fibronectin/integrin-, cAMP/PKAand rhoa-mediated intracellular signal transduction pathways. rho activity was detected by pull-down assay. caMP concentration was measured by radioimmunoassay. The distribution of the PKa catalytic subunit and the phosphorylation of vasodilator-stimulated phosphoprotein (VaSP) were detected by fluorescence microscopy and Western blotting, respectively, to examine the activation of PKa. caMP-mediated gene expression activity was analyzed using a luciferase reporter gene assay. The results revealed that, in SGc-7901 cells, soluble fibronectin increased RhoA activity and blocked the inhibition of rhoa activity by caMP/PKa. The caMP level, which was increased by forskolin and pertussis toxin, was decreased by fibronectin. The nuclear location of the PKA catalytic unit, the phosphorylation of VaSP and caMP response element (cre)-directed reporter gene expression induced by forskolin were blocked by fibronectin. However, fibronectin did not block VaSP phosphorylation or cre-directed reporter gene expression induced by cAMP. These data suggest that fibronectin/ integrin induces rhoa activation through the inhibition of caMP/PKa signal transduction. The possible point of action of fibronectin/integrin is adenylate cyclase.

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Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Cited by 17 publications

References 44 publications

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Expression of hypoxia-inducible factor-1α during ovarian follicular growth and development in Sprague-Dawley rats

Fibronectin increases RhoA activity through inhibition of PKA in the human gastric cancer cell line SGC-7901

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