Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase

Song, Hongyan; Song, Xiaoling; Xu, Lixin; Ren, Yan; Shah, Muhammad Ali; Li, Xiangrui

doi:10.1016/j.vetpar.2010.06.030

Cited by 31 publications

(27 citation statements)

References 49 publications

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“…The spleen is an important immunological organ in the chicken and has been successfully utilized to detect differences in cytokine gene expression after immunization Marek's disease vaccines and DNA vaccines [30-34]. The selection of vaccination at 2 weeks of age has also been successfully utilized to detect splenic expression differences [31], with several other vaccination times used successfully: day 18 of incubation, 1 day of age, and 2 weeks of age with a 4 week booster [30,32-34]. The time period selected between immunization and sampling can greatly impact the ability to detect expression differences, with later sampling times, 10 days post vaccination, showing reduced expression differences compared to earlier sampling times [31,33], though detection 26 days post vaccination is still possible [34].…”

Section: Discussionmentioning

confidence: 99%

Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

et al. 2011

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BackgroundAvian pathogenic Escherichia coli (APEC) is detrimental to poultry health and its zoonotic potential is a food safety concern. Regulation of antimicrobials in food-production animals has put greater focus on enhancing host resistance to bacterial infections through genetics. To better define effective mechanism of host resistance, global gene expression in the spleen of chickens, harvested at two times post-infection (PI) with APEC, was measured using microarray technology, in a design that will enable investigation of effects of vaccination, challenge, and pathology level.ResultsThere were 1,101 genes significantly differentially expressed between severely infected and non-infected groups on day 1 PI and 1,723 on day 5 PI. Very little difference was seen between mildly infected and non-infected groups on either time point. Between birds exhibiting mild and severe pathology, there were 2 significantly differentially expressed genes on day 1 PI and 799 on day 5 PI. Groups with greater pathology had more genes with increased expression than decreased expression levels. Several predominate immune pathways, Toll-like receptor, Jak-STAT, and cytokine signaling, were represented between challenged and non-challenged groups. Vaccination had, surprisingly, no detectible effect on gene expression, although it significantly protected the birds from observable gross lesions. Functional characterization of significantly expressed genes revealed unique gene ontology classifications during each time point, with many unique to a particular treatment or class contrast.ConclusionsMore severe pathology caused by APEC infection was associated with a high level of gene expression differences and increase in gene expression levels. Many of the significantly differentially expressed genes were unique to a particular treatment, pathology level or time point. The present study not only investigates the transcriptomic regulations of APEC infection, but also the degree of pathology associated with that infection. This study will allow for greater discovery into host mechanisms for disease resistance, providing targets for marker assisted selection and advanced drug development.

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Section: Discussionmentioning

confidence: 99%

Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

et al. 2011

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“…Recently, many publications demonstrated that co-delivery of cytokines as adjuvants could enhance the potential for DNA vaccines to induce strong humoral and cellular immunity [13,15,16]. In this study, the cSZ-JN1 could stimulate high levels of IL-4 and TGF-␤ and resulted in an ACI more than 160 in the protective experiment.…”

Section: Discussionmentioning

confidence: 68%

“…IL-4, a Th2-type cytokine, is known to regulate humoral immunity and to function more effectively as helper for B-cell activation [35,36]. TGF-␤, as a regulatory (iTreg) cytokine, plays important role in the immune response against Eimeria antigens [16]. So, in this research, we used these cytokines as marks to select antigens with different functions.…”

Section: Discussionmentioning

confidence: 99%

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Identification and molecular characterization of a novel antigen of Eimeria acervulina

Zhu

Ren

Wang

et al. 2012

Molecular and Biochemical Parasitology

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“…It has also been found that DNA vaccines or recombinant antigen can provoke both humoral and cell-mediated immune responses [9,10], and co-delivery of cytokines as adjuvants could enhance the potential for DNA vaccines or recombinant antigen to induce broad and long-lasting humoral and cellular immunity [11].…”

Section: Introductionmentioning

confidence: 99%

Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge

Huang

Zhang

et al. 2015

Parasitology International

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Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase

Cited by 31 publications

References 49 publications

Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

Identification and molecular characterization of a novel antigen of Eimeria acervulina

Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge

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