Changes of cytosolic calcium and contractility of young rat vas deferens by acute treatment with amphetamine, fluoxetine or sibutramine

Jurkiewicz, Neide Hyppolito; Silva, Edilson Dantas da; Souza, Bruno Palmieri de; Verde, Luciana Ferreira; Pereira, Janaína Drawanz; Sobrinho, Cairo Mendes; Smaili, Soraya Soubhi; Caricati‐Neto, Afonso; Miranda-Ferreira, Regiane; Jurkiewicz, Aron

doi:10.1016/j.ejphar.2012.07.027

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…Furthermore, sibutramine promotes increased tension developed in the epididymal duct, mediated by calcium ions influx, as shown by the nifedipine assays. This finding was corroborated by a previous work [37], in which sibutramine pretreatment promoted an influx of calcium ions and increased the tension developed in the vas deferens.…”

Section: Discussionsupporting

confidence: 87%

“…Currently, there are some reports in the literature on the direct and indirect effects of sympathomimetic drugs on reproductive organs [12], [20], [34], [35], [36], [37], however there are no extensive studies on the mechanisms involved. In this study, we report pharmacological mechanisms by means sibutramine, a serotonin-NE reuptake inhibitor, promotes increased NE effects in the rat epididymal duct and ventral prostate and promotes NE depletion in the epididymis.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in the mechanical activity of the distal epididymal cauda was abolished in the presence of nifedipine, a L-type calcium channel blocker [45]. As previous studies showed that sibutramine blocks voltage-dependent K + channel [12], [20], [37], [46], [47] it is possible that the resulting membrane depolarization opens L-type calcium channels, allowing influx of extracellular calcium and contracting the distal epididymal cauda.…”

Section: Discussionmentioning

confidence: 99%

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Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine

et al. 2013

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Sperm acquire motility and fertility capacity during epididymal transit, under the control of androgens and sympathetic innervations. It is already known that the acceleration of epididymal sperm transit time can lead to lower sperm quality. In a previous work we showed that rats exposed to the anorexigen sibutramine, a non-selective serotonin-norepinephrine reuptake inhibitor, presented faster sperm transit time, lower epididymal sperm reserves and potentiation of the tension of epididymal duct to norepinephrine exposed acutely in vitro to sibutramine. In the present work we aimed to further investigate pharmacological mechanisms involved in these alterations and the impact on rat sperm quality. For this, adult male Wistar rats were treated with sibutramine (10 mg/kg/day) or vehicle for 30 days. Sibutramine decreased final body, seminal vesicle, ventral prostate and epididymal weights, as well as sperm transit time in the epididymal cauda. On the contrary of the in vitro pharmacological assays, in which sibutramine was added directly to the bath containing strips of distal epididymal cauda, the ductal tension was not altered after in vivo sub-chronic exposure to sibutramine. However, there is pharmacological evidence that the endogenous epididymal norepinephrine reserves were reduced in these animals. It was also shown that the decrease in prostate weight can be related to increased tension developed of the gland, due to sibutramine sympathomimetic effects. In addition, our results showed reduced sperm quality after in utero artificial insemination, a more sensitive procedure to assess fertility in rodents. The epididymal norepinephrine depletion exerted by sibutramine, associated with decreases in sperm transit time, quantity and quality, leading to reduced fertility in this experimental model, reinforces the concerns about the possible impact on fertility of man taking sibutramine as well as other non-selective serotonin-norepinephrine reuptake inhibitors, especially considering the lower reproductive efficiency of humans compared to males of other species.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine

et al. 2013

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“…This effect results in increased level of serotonin in the synaptic cleft, facilitating its binding to its postsynaptic receptor. However, in the past two decades, preclinical studies demonstrated that fluoxetine, and other SSRIs, also exert significant other cellular effects that are independent of their ability to inhibit serotonin reuptake and involve antagonism of serotonin receptors and inhibition of L-type and T-type voltage-dependent Ca 2+ channels, as well as other ion channels (Pacher et al, 1998; Pacher et al, 1999a; Ungvari et al, 1999; Deak et al, 2000; Pacher et al, 2000; Ungvari et al, 2000; Terstappen et al, 2003; Traboulsie et al, 2006; Jurkiewicz et al, 2012; Gobin et al, 2015). Importantly, fluoxetine, as well as other SSRIs, was shown to exert significant vascular effects, including inhibition of pressure-induced myogenic constriction of blood vessels (Pacher et al, 1999a; Ungvari et al, 1999; Ungvari et al, 2000) ( Figure 1 ).…”

Section: Vascular Side Effects Of Fluoxetine and Other Ssris: Potentimentioning

confidence: 99%

Potential Adverse Cardiovascular Effects of Treatment With Fluoxetine and Other Selective Serotonin Reuptake Inhibitors (SSRIs) in Patients With Geriatric Depression: Implications for Atherogenesis and Cerebromicrovascular Dysregulation

et al. 2019

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Late life depression is an important public health problem, which associates with increased risk of morbidity and mortality. Selective serotonin reuptake inhibitors (SSRIs), including fluoxetine, are often prescribed to treat geriatric depression. There is increasing evidence that fluoxetine and other SSRIs exert a wide range of cardiovascular side effects. Furthermore, there is evidence that aging may increase plasma level of SSRIs. In this overview, the potential role of side effects of treatment with fluoxetine and other SSRIs in the pathogenesis of age-related cardiovascular diseases, including atherogenesis, cardiac pathologies, and cerebromicrovascular impairment, is discussed.

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Effects of in vitro, acute and chronic treatment with fluoxetine on the sympathetic neurotransmission of rat vas deferens

Pedroso

Souza

Jurkiewicz

et al. 2017

Autonomic Neuroscience

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Changes of cytosolic calcium and contractility of young rat vas deferens by acute treatment with amphetamine, fluoxetine or sibutramine

Cited by 7 publications

References 40 publications

Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine

Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine

Potential Adverse Cardiovascular Effects of Treatment With Fluoxetine and Other Selective Serotonin Reuptake Inhibitors (SSRIs) in Patients With Geriatric Depression: Implications for Atherogenesis and Cerebromicrovascular Dysregulation

Effects of in vitro, acute and chronic treatment with fluoxetine on the sympathetic neurotransmission of rat vas deferens

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