Changes of d-Limonene Content in Three Citrus Species during Fruit Development.

Hara, Masakazu; Kishimoto, Mayuko; Kuboi, Toru

doi:10.3136/fstr.5.80

Cited by 11 publications

(5 citation statements)

References 10 publications

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“…Among the tested, six terpenes, including ρ-cymene, limonene (+), limonene (−), linalool, α-pinene (+), and β-pinene (−) were shown to relieve the rough eye phenotype when fed to Previously, we reported that ectopic expression of Aβ42 in neurons decreased the survival rate of flies during development. [21][22][23] Therefore, we conducted a secondary screening to observe if feeding the selected terpenes would increase the survival rate of the flies expressing Aβ42 in neurons during the developmental stage. The survival rate of the Drosophila was measured in duplicate after being fed with low concentrations (50 µg/mL) and high concentrations (200 µg/mL) of the six terpenes selected during the first screening.…”

Section: Screening the Terpenes That Alleviate The Phenotypes Of The mentioning

confidence: 99%

Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a <i>Drosophila</i> Model of Alzheimer’s Disease

Shin

Liu

Choi

et al. 2020

Biological & Pharmaceutical Bulletin

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Forest bathing is suggested to have beneficial effects on various aspects of human health. Terpenes, isoprene based-phytochemicals emitted from trees, are largely responsible for these beneficial effects of forest bathing. Although the therapeutic effects of terpenes on various diseases have been revealed, their effects on neuronal health have not yet been studied in detail. Here, we screened 16 terpenes that are the main components of Korean forests using Drosophila Alzheimer's disease (AD) models to identify which terpenes have neuroprotective effects. Six out of the 16 terpenes, ρ-cymene, limonene (), limonene (), linalool, α-pinene (), and β-pinene (), partially suppressed the beta amyloid 42 (Aβ42)-induced rough eye phenotype when fed to Aβ42-expressing flies. Among them, limonene () restored the decreased survival of flies expressing Aβ42 in neurons during development. Limonene () treatment did not affect Aβ42 accumulation and aggregation, but did cause to decrease cell death, reactive oxygen species levels, extracellular signal-regulated kinase phosphorylation, and inflammation in the brains or the eye imaginal discs of Aβ42-expressing flies. This neuroprotective effect of limonene () was not associated with autophagic activity. Our results suggest that limonene () has a neuroprotective function against the neurotoxicity of Aβ42 and, thus, is a possible therapeutic reagent for AD.

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Section: Screening the Terpenes That Alleviate The Phenotypes Of The mentioning

confidence: 99%

Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a <i>Drosophila</i> Model of Alzheimer’s Disease

Shin

Liu

Choi

et al. 2020

Biological & Pharmaceutical Bulletin

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“…[55][56][57] Studies have indicated that the flowers and fruits of citrus synthesize and release a large amount of d-limonene during the flowering stage (February to April) 58 and fruit maturity stage (August to November). 59 Unexpectedly and interestingly, B. minax uses d-limonene as a sex pheromone, but the presence of large amounts of d-limonene in citrus orchards interferes with male directional selection of females. With one generation per year, the emergence and oviposition of adult B. minax mainly occurs from May to July, and a small number of female flies stay in the citrus orchards until September.…”

Section: Discussionmentioning

confidence: 99%

“…D‐limonene is widely distributed in the leaves, flowers and fruits of plants, especially in citrus fruits 55–57 . Studies have indicated that the flowers and fruits of citrus synthesize and release a large amount of d‐limonene during the flowering stage (February to April) 58 and fruit maturity stage (August to November) 59 . Unexpectedly and interestingly, B. minax uses d‐limonene as a sex pheromone, but the presence of large amounts of d‐limonene in citrus orchards interferes with male directional selection of females.…”

Section: Discussionmentioning

confidence: 99%

Exploration of D‐limonene as a sex pheromone for males of Bactrocera minax (Diptera: Tephritidae)

Cheng,

Wen,

et al. 2023

Pest Management Science

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BACKGROUNDBactrocera minax is a devastating pest of citrus fruits. However, there have been no effective control measures before. Few reports on the sex pheromones of B. minax are available.RESULTSIn this study, nine of the volatile compounds in adult females were identified using headspace solid‐phase microextraction (HS‐SPME) in combination with gas chromatography–mass spectrometry (GC–MS). Among them, d‐limonene, caprolactam, 2‐Nitro‐1H‐imidazole, and creatinine could evoke antennal responses in males. Field bioassays showed that only d‐limonene could lure male flies, with a relative lure rate of 78.18% in all tested samples, which was significantly higher than that of paraffin oil control, while all volatile compounds did not have any lure effective to female flies. Moreover, d‐limonene was diluted with paraffin oil into differential concentrations, the lure effect on males was better at 100, 500, and 800 μL d‐limonene mL−1 than pure d‐limonene (1000 μL mL−1). The relative male lure rate of d‐limonene at 100 μL mL−1 was 85.88%, which was significantly higher than that of food−baits (14.12%) on day 3. However, d‐limonene was unattractive to female and male Bactrocera dorsalis and Zeugodacus tau. Further kinetic analysis showed that female adults released d‐limonene around 15‐day post eclosion. Electroantennography 1 results showed that 500 μL mL−1 d‐limonene evoked the strongest responses to antennae of 10‐ to 25‐day‐old male flies.CONCLUSIONOur results indicated that d‐limonene could be a sex pheromone from female flies of B. minax, and it could be used as a male‐specific sex attractant for B. minax. © 2023 Society of Chemical Industry.

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“…In the HO and HP populations, discs were sampled from the intact leaves at 0 DPI, namely before wounding. Volatile organic compounds were extracted from the samples as described in Hara et al (1995). The GC-MS analysis was conducted according to the methods of Shimada et al (2017).…”

Section: Quanti Cation Of Linalool In Leaf Samples From Eld-grown Tre...mentioning

confidence: 99%

Biological and molecular characterization of linalool-mediated field resistance against Xanthomonas citri subsp. citri in citrus trees

et al. 2021

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The biological and molecular traits of the Ponkan mandarin (Citrus reticulata Blanco) were characterized in an investigation of the mechanisms of field resistance against citrus canker disease caused by the bacterial pathogen, Xanthomonas citri subsp. citri (Xcc). Various conventional citrus varieties that show diverse responses to Xcc were investigated, and the temporal changes in Xcc titer in response to linalool concentrations among the varieties revealed differences in Xcc proliferation trends in the inoculated leaves of the immune, field-resistant, and susceptible varieties. In addition, increased linalool accumulation was inversely related to Xcc titers in the field-resistant varieties, which is likely caused by host-pathogen interactions. Quantitative trait locus (QTL) analysis using the F1 population of the resistant Ponkan mandarin and susceptible ‘Harehime’ (‘E-647’ × ‘Miyagawa-wase’) cultivar revealed that linalool accumulation and Xcc susceptibility QTLs overlapped. These results provide novel insights into the molecular mechanisms of linalool-mediated field resistance to Xcc, and suggest that high linalool concentrations in leaves has an antibacterial effect and become a candidate-biomarker target for citrus breeding to produce seedlings with linalool-mediated field resistance against Xcc.

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Changes of d-Limonene Content in Three Citrus Species during Fruit Development.

Cited by 11 publications

References 10 publications

Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a <i>Drosophila</i> Model of Alzheimer’s Disease

Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a <i>Drosophila</i> Model of Alzheimer’s Disease

Exploration of D‐limonene as a sex pheromone for males of Bactrocera minax (Diptera: Tephritidae)

Biological and molecular characterization of linalool-mediated field resistance against Xanthomonas citri subsp. citri in citrus trees

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