Changes of Estradiol and Lipid Concentrations in Serum and Liver Fatty Acid of Maturing Hen and Cockerel

Fatty Acid Compositions of Triacylglycerols and Phospholipids in Hen Liver Lipid Before and After Sexual Maturity

“…The average age at first egg was 147 days of age, while 50% egg production was reached at 149 days of age. Total lipid of the hen liver significantly increased after the onset of egg production (Fujii et al, 1984). When the lipid was separated into neutral lipids and phospholipids by silica gel column chromatography, the ratio of neutral lipids to phospholipids was about 62:38 in the young nonlaying hen liver (97 days of age).…”

“…They were subjected to 14 hr light/day throughout this experiment and were fed the experimental diet (Fujii et al, 1984), which contained 17.9% crude protein, 3.2% crude fat, 4.2% crude ash, 1.6% crude fiber, and 3132 kcal/kg metabolizable energy (air-dry basis). The fatty acid composition (%, w/w) of the lipid in the diet was (carbon number: double bonds): 14:0, .1%; 16:0, 11.3%; 16:1, .4%; 17:0, .1%; 18:0, 2.1%; 18:1, 27.0%; 18:2, 56.2%; 18:3, 2.0%; 20:0, .5%; and 20:1, .3%.…”

“…The areas corresponding to triacylglycerols and phospholipids were scraped off into vials, and the methyl esters of the fatty acids were prepared as described previously (Fujii et al, 1984). The fatty acid esters were analyzed by a Shimadzu GC-4B gas chromatograph with an FID-4C flame ionization detector (conditions: 3 mm X 3 m stainless steel column packed with Advance-DS, 80-100 mesh, support Chromosorb W (Wako Chemical Co., Osaka), column temperature 210 C, sample inlet temperature 250 C, carrier gas nitrogen (N 2 ) with a flow rate of 44 mg/min).…”

“…This increase was due almost entirely to an accumulation of triacylglycerols (Balnave, 1968). We previously described (Fujii et al, 1984) increases in liver lipid and serum estrogen and marked changes in fatty acid composition of liver and serum lipids at the onset of egg production. However, there are few reports concerning the changes in the fatty acid composition of the triacylglycerols and phospholipids of the hen liver before and after the onset of lay.…”

Appearance of Triacylglycerol Lipase in Egg Yolk Sac of Japanese Quail during Embryonic Development, Its Partial Purification and Some Properties

“…Commercial triacylglycerols used as substrate for lipase were purified by Florisil column chromatography,7) and the purity was monitored by silica gel G-60 thin-layer chromatography as described elsewhere. 8 ) Isoelectric focusing was done using 1% carrier ampholyte (vjv) covering the pH range of 4N6 in a 110-ml column, as directed in the instruction manual supplied by LKB. The voltage was maintained at 400 V for the first 10 hr and at 1000 V for the next 30 hr.…”