Changes of Hematological and Hemorheological Parameters in Rabbits with Hypercholesterolemia

Tanczos, Bence; Somogyi, Viktória; Bombicz, Mariann; Juhász, Béla; Németh, Norbert; Deák, Ádám

doi:10.3390/metabo11040249

Cited by 8 publications

(8 citation statements)

References 58 publications

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“…It has been shown that hypercholesterolemia elevated MCV values in human subjects (Prasad, 2010), although other studies indicated lack of any effect of hypercholesterolemia (Choi and Pai, 2004) or even an inverse correlation (Bunyaratvej et al, 2000). Additionally, a recent study in rabbits indicated that MCV value of RBCs was significantly elevated in hypercholesterolemia (Tanczos et al, 2021). MCV values were significantly elevated in ApoE/LDLR −/− mice, what stands in line with different studies in murine models (Holm et al, 2002;Hoekstra et al, 2013), as well as with our previous work (Dybas et al, 2020).…”

Section: MCV Mch and Mchcsupporting

confidence: 89%

Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice

Alcicek

Mohaissen²,

Bulat³

et al. 2022

Front. Physiol.

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In this study for the first time, we investigated the correlation between sex-specific differences in adenosine triphosphate (ATP) levels in red blood cells (RBCs) and their mechanical, biochemical, and morphological alterations during the progression of atherosclerosis in ApoE/LDLR double-deficient (ApoE/LDLR−/−) mice. Our results indicate that both sex and age affect alterations in RBCs of both ApoE/LDLR−/− and C57BL/6J mice. When compared with male RBCs, female RBCs were characterized by lower basal ATP and mean corpuscular hemoglobin concentration (MCHC), higher hemoglobin concentration (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), deformability, and phosphatidylserine (PS) exposure levels, regardless of age in both, ApoE/LDLR−/− and C57BL/6J mice. ApoE/LDLR−/− mice compared with age-matched controls showed lower basal ATP levels regardless of age and sex. Intracellular ATP level of RBCs was decreased solely in senescent female C57BL/6J mice, while it was elevated in males. Basal extracellular ATP levels were 400 times lower than corresponding intracellular level. In conclusion, basal ATP levels, RBC morphology, deformability, PS exposure levels alterations are sex-dependent in mice. Changes in basal ATP levels were correlated with PS exposure and trends of changes in MCV. Trends of changes of the most RBC parameters were similar in both sexes of ApoE/LDLR−/− mice compared with age-matched controls; however, their kinetics and levels vary greatly between different stages of disease progression.

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Section: MCV Mch and Mchcsupporting

confidence: 89%

Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice

Alcicek

Mohaissen²,

Bulat³

et al. 2022

Front. Physiol.

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“…Figure c shows that the left average RBC numbers on the 5, 15%, and bilayer porous membranes were 2.5 × 10 4 , 1.9 × 10 4 , and 2.1 × 10 4 per square centimetre, respectively. The intercepted RBCs by the three porous membranes were far less than the quantity of the RBCs in rabbit’s blood (∼5.2–7.6 × 10 9 /mL) . Therefore, the characteristics of the porous membranes in intercepting RBCs can be neglected.…”

Section: Resultsmentioning

confidence: 97%

“…The intercepted RBCs by the three porous membranes were far less than the quantity of the RBCs in rabbit's blood (∼5.2−7.6 × 10 9 /mL). 46 Therefore, the characteristics of the porous membranes in intercepting RBCs can be neglected. In addition, SEM images showed that no other cells (e.g., neutrophils, lymphocytes, and monocytes) adhered on the surfaces of the 5 and 15% porous membranes, indicating that the porous 90CA10SF membranes hardly intercept white cells.…”

Section: Hemocompatible and Antifouling Verification Of The Porous 90...mentioning

confidence: 99%

Construction of Porous Bilayer Cellulose Acetate/Silk Fibroin Membranes for Bloodstream Infection Treatment

Cai

Hong

2023

ACS Appl. Eng. Mater.

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The removal of harmful substances from blood is always a technological challenge to treat bloodstream infection (BSI). To this end, we explore the use of a phase-inversion and bilayer membrane cast process to construct porous bilayer cellulose acetate (CA)/silk fibroin (SF) composite membranes consisting of a big porous membrane (Max/ Average/Min pore size 3.37:1.57:0.63 μm) and a small porous membrane (Max/Average/Min pore size 0.48:0.26:0.11 μm) to capture bacteria and endotoxins. Experiments demonstrated that the concentrations of CA and SF and the weight ratio of CA and SF played important roles in preparing porous CA/SF membranes with desirable pore size and high mechanical strength. In vitro and blood contact experiments demonstrated that the as-prepared porous CA/SF membranes had good hemocompatibility and antifouling properties. In vitro and harmful substance-contained blood contact experiments demonstrated that the porous bilayer CA/SF composite membranes could effectively capture high (10 6 CFU/mL bacteria and 300 EU/mL endotoxins) and low (10 2 CFU/mL bacteria and 5 EU/mL endotoxins) concentrations of Escherichia coli, Staphylococcus aureus, and endotoxins, and the ability of the porous bilayer CA/SF composite membranes to capture bacteria and endotoxins could be attributed to the big size of the pores and the affinity of the bacteria/endotoxins to CA/SF. Our work shows that porous bilayer CA/SF membranes have the potential for the treatment of BSI.

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“…Given that RBCs are incapable of performing biosynthesis due to the lack of organelles, it is opportune to mention that the membrane lipid composition of RBCs depends on the exchange with the plasma lipids [12][13][14]. In support of this, hemorheological changes have been demonstrated in rabbits with hypercholesterolemia [33]. Other researchers have proved that RBCs are sensitive to plasma lipids [34][35][36].…”

Section: Discussionmentioning

confidence: 95%

Glycerophospholipids in Red Blood Cells Are Associated with Aerobic Performance in Young Swimmers

Silva,

Bertolucci,

Scariot

et al. 2024

Nutrients

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This study aimed to characterize the composition of lipids in the red blood cells (RBCs) of adolescent swimmers and correlate this lipidome with the aerobic performance of the athletes. Five experimental assessments were performed by 37 adolescent swimmers. During the first session, the athletes went to the laboratory facility for venous blood sampling. The critical velocity protocol was conducted over the 4 subsequent days to measure aerobic performance (CV), comprising maximal efforts over distances of 100, 200, 400, and 800 m in a swimming pool. RBCs were obtained and extracted for analysis using the liquid chromatography—high resolution mass spectrometry untargeted approach. A total of 2146 ions were detected in the RBCs, of which 119 were identified. The enrichment pathway analysis indicated intermediary lipids in the glycerophospholipid, glycerolipid, sphingolipid, linoleic acid, and alpha-linolenic metabolisms, as well as pentose and glucuronate interconversions. A significant impact of the intermediary lipids was observed for the glycerophospholipid metabolism, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), 1-acyl-sn-glycero-3-phosphocholine, sn-glycerol 3-phosphate, and phosphatidic acid. Inverse and significant associations were observed for PE 18:2/18:3 (r = −0.39; p = 0.015), PC 18:3/20:0 (r = −0.33; p = 0.041), and phosphatidic acid 18:0/0:0 (r = −0.47; p = 0.003) with aerobic performance. Swimmers who exhibited higher levels of aerobic performance also had the lowest abundance of PE, PC, and phosphatidic acid.

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Changes of Hematological and Hemorheological Parameters in Rabbits with Hypercholesterolemia

Cited by 8 publications

References 58 publications

Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice

Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice

Construction of Porous Bilayer Cellulose Acetate/Silk Fibroin Membranes for Bloodstream Infection Treatment

Glycerophospholipids in Red Blood Cells Are Associated with Aerobic Performance in Young Swimmers

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