Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells

Renner, Kathrin; Amberger, Albert; Konwalinka, G; Kofler, Reinhard; Gnaiger, Erich

doi:10.1016/s0167-4889(03)00105-8

Cited by 111 publications

(72 citation statements)

References 38 publications

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“…Previous investigations have shown that dexamethasone (Dex), a synthetic glucocorticoid, reduces the survival and induces the apoptosis of thymocytes, leukemia and insulin-secreting cells (5)(6)(7)(8)(9), and the effect is mediated through the release of mitochondrial cytochrome c (5,6), the activation of caspases and generation of reactive oxygen species (8), the inhibition of nuclear factor-κB (9,10), and/or the inhibition of phosphorylation of insulin receptor substrate-2 and protein kinase B (PKB) (11). A large body of evidence also demonstrates that Dex treatment leads to alteration of the expression and/or activities of many intracellular signaling proteins (12)(13)(14), including mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) and the family of MAPKs, which are involved in gene expression, cell survival and/or apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity

Kim

Park

Kim³

et al. 2013

International Journal of Molecular Medicine

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Abstract. Excessive and/or long-term glucocorticoid therapy reduces β-cell mass and induces hyperglycemia, which contribute to the development of steroid-induced diabetes. Ceriporia (C.) lacerata is one of the white-rot fungi and has been used in bioremediations, such as lignocellulose degradation, in nature. The pharmacologic effect of C. lacerata on steroid-induced β-cell toxicity is not known. In this study, we evaluated the effect of a crude extract from a submerged cultivation of C. lacerata on the survival and apoptosis of INS-1 rat insulin-secreting cells exposed to dexamethasone (Dex), a synthetic diabetogenic glucocorticoid. Treatment with the C. lacerata crude extract (CLCE) largely blocked the Dex-induced reduction in survival and apoptosis of INS-1 cells. Moreover, CLCE treatment inhibited Dex-induced protein kinase B (PKB) dephosphorylation without affecting Dex-induced extracellular signal-regulated protein kinase-1/2 dephosphorylation and MKP-1 upregulation. Importantly, the protective effect of CLCE on Dex-induced cytotoxicity in INS-1 cells was attenuated by LY294002, an inhibitor of PI3K/PKB. CLCE treatment, however, did not protect the INS-1 cells from the cytotoxic effects triggered by other insults, such as interleukin-1β (an inflammatory cytokine), streptozotocin (a diabetogenic drug), thapsigargin (a calcium mobilizing agent), and tunicamycin (an ER stress inducer).Collectively, these findings demonstrate for the first time the ability of CLCE to specifically protect INS-1 cells from Dex-induced cytotoxicity through the modulation of the PI3K/ PKB pathway. It is suggested that CLCE may be applied for the prevention and/or treatment of steroid diabetes in which reduction of β-cell survival and induction of β-cell apoptosis play pathogenic roles.

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Section: Introductionmentioning

confidence: 99%

Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity

Kim

Park

Kim³

et al. 2013

International Journal of Molecular Medicine

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“…To reduce the impact of this factor, some authors propose to express the tissue respiration data per citrate synthase activity (Renner et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial function and aerobic capacity assessed by high resolution respirometry in Thoroughbred horses

Serteyn

Ceusters

Nonnenmacher

et al. 2016

CEP

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During the initial stages of training of young Thoroughbred horses, low intensity exercise is employed to increase aerobic capacity. High Resolution Respirometry (HRR) allows the determination of aerobic capacities in small samples of permeabilised muscle fibres. The aim of the study was to measure the mitochondrial function by HRR in Thoroughbred horses, to compare these values to Warmblood horses and to evaluate the effect of a 10-weeks training period. The mitochondrial function was measured by HRR using different substrate-uncoupler protocols (SUIT 1 and 2) in muscle microbiopsies from two groups of untrained horses: 17 Warmblood and 8 Thoroughbred and in the group of 8 Thoroughbred horses before and after a 10-week training period. The SUIT1 protocol employed to compare the two groups of horses showed that in Thoroughbred horses, the mean values for oxygen flux expressed as tissue mass-specific respiration were significantly higher for complex I (CI) Glutamate+Malate , CI + complex II, and maximum electron transport capacities (ETSmax) than the mean values measured in Warmblood horses. The SUIT 1 and SUIT 2 protocols revealed large differences among Thoroughbred horses before and after training. The SUIT 2 protocols showed a significant difference for the complex I activity before and after training but only when the oxygen flux was expressed as percentage of ETSmax. This study shows the interest of HRR in equine sport medicine and exercise physiology, but shows that the technique requires further refinement. Indeed significant differences have been shown between the Thoroughbred and the Warmblood horses highlighting the need to have baseline data for each breed. The Thoroughbred horses had globally a high oxidative phosphorylation capacity with an increase of CI activity induced by an aerobic training program.

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“…The cytotoxicity induced by EFTB was determined by the quantitative estimation of cytoplasmic lactate dehydrogenase (LDH) leaked into the culture medium by the method of Renner et al, 2003(Renner et al, 2003. Briefly, after the exposure of cell lines to the different concentrations of EFTB, the culture medium was aspirated and centrifuged at 4000 rpm for 10 min to get cell free supernatant.…”

Section: Neutral Red Cell Viability Assay:-mentioning

confidence: 99%

Cytotoxicity Evaluation of Bioactive Fraction From Terminalia Bellirica(gaertn.)roxb. Fruits in L929 Cells.

Jayesh¹,

Helen²,

Vysakh³

et al. 2017

IJAR

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Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells

Cited by 111 publications

References 38 publications

Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity

Crude extract of Ceriporia lacerata has a protective effect on dexamethasone-induced cytotoxicity in INS-1 cells via the modulation of PI3K/PKB activity

Mitochondrial function and aerobic capacity assessed by high resolution respirometry in Thoroughbred horses

Cytotoxicity Evaluation of Bioactive Fraction From Terminalia Bellirica(gaertn.)roxb. Fruits in L929 Cells.

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