Changes of oxygen status of hair cells for breast cancer presence

Abstract

The results of differences of wool oxygen status of healthy and cancer carrier mice with spontaneous cancer tumor are represented herein. The investigation was carried out by electronic microscopy and laser-flash photolysis. In the work, two types of breast tumors were researched. Small tumor (nearly 0.5 ÷ 1 cm) was detected by palpation and was well encapsulated. Large tumor was operated when tumor had been disintegrated. It is shown that wool of healthy mouse has dense structure; external layer with large scales is pronounced. At the time, wool of mice with cancer tumor is thinner; external and internal layers are destroyed. Length of scales is shorter. There is difference even when tumor diameter is small. Oxygen concentration increases for cancer present, and it is related to reactive oxygen species rising within external layer. Reactive oxygen species have most distribution in general oxygen concentration in external cell layer. At the time, its increase in internal layer is insignificant. The obtained results can be used to improve optical techniques of biomedical diagnostics of cancer diseases.

Investigation of Hair Microstructure Changes in Patients with Malignant Tumours by Optical Techniques

“…This leads to decreased nitrogen concentration. Moreover, as was proved in our previous work [10], data of external and internal layer can be experimentally observed due to the destruction of hair external layer in cancer patients.…”

“…The presence of malignant tumour leads to the increase of this ratio, on account of the decrease in nitrogen and the consequent decrease in peptide concentration. This dependence was demonstrated in hair samples in mice with spontaneous breast tumour [10]. Table 2 The results of quantitative analysis of nitrogen and oxygen concentrations in the investigated groups.…”

Nature of phosphorescence kinetics of xanthene dyes in biological media

“…The basic objects of the disscussion will be curves of the dyes by which hair cell suspentions were colored [5]. For additional experiments two types of dye solutions were prepeared after mixing the water solution of the dye with glucose water solution (0.1% weight).…”

“…For example, Penzkofer and coauthors [3] investigated the phosphorescence of xanthene dyes dissolved in gelatin, starch and chitosan and also showed its non-monoexponential type. At the time, in our previous works [4,5], this dependence was represented for samples with hair and mammary cells.…”

“…Figure 1. Typical phosphorescence kinetics of xanthene dyes in different media: eosine in cells of wool of healthy (1) and cancer carrier mice with small (2) and large tumor (3), in glucose without cells (4)[5], rose bengal (5) and erythrosine (6) in collagenase[7].…”