The study aimed to determine the minimum inhibitory concentration of
Aloe vera
extracts obtained by different extraction methods on eight strains from five different pathogens (
Pseudomonas fluorescens
,
Listeria monocytogenes
,
Salmonella
spp.,
Escherichia coli
and
Staphylococcus aureus
) in the first phase and utilize
Aloe vera
extract in sausage processing in the second phase. Sausages were evaluated for thiobarbituric acid reactive substances (TBARS), physicochemical and microbiological properties. The first phase results indicated that the highest inhibition was determined in
Listeria monocyctogenes
472 regardless of tested
Aloe vera
extract doses and extraction methods (Tukey HSD,
P
< 0.05). The highest
Listeria monocyctogenes
472 levels reached was 0.38 log
10
cfu/g. The second phase results revealed that
Aloe vera
containing treatments of sausage had lower pH than others after storage (ANOVA,
P
< 0.05). Dry matter, protein, fat and ash contents of sausage dough increased in all treatments after fermentation (ANOVA,
P
< 0.05). After 30 d storage, 48 and 45% TBARS reductions were obtained in sausages with only nitrite and those with only
Aloe vera
extract compared to control respectively. The lowest TBARS (68% reduction) were obtained in
Aloe vera
extract and nitrite incorporated sausages (Tukey HSD,
P
< 0.05). Result showed that the use of
Aloe vera
extract and nitrite combination in sausage formulation is a useful approach to control lipid oxidation in the product.
Supplementary information
The online version contains supplementary material available at 10.1007/s13197-021-05183-5.