Changes of physiological and biochemical characteristics of rat erythrocytes after blood loss

Маслова, М. Н.; Kazennov, Andrey; Katyukhin, L. N.; Novozhilov, A. V.; Skverchinskaya, Elisaveta; Tavrovskaya, T. V.

doi:10.1134/s0022093007050064

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the composition of the lipid vesicles is similar to that of the cell membrane, as it was specifically designed to be unstable in order to fuse and integrate with cell membranes on contact. Because of these similarities, the integration of lipid vesicles within the cell membrane can potentially ameliorate the cell membrane structural abnormalities such as fluidity, which, as demonstrated in a number of studies, is known to occur with haemorrhage [29][30][31]. Second, the observed favorable effects of the lipid vesicles might relate to the ability of the phospholipid components of the vesicles to stimulate the Na+/K+-ATPase [32].…”

Section: Discussionmentioning

confidence: 99%

Improved Post-Resuscitation Survival Time with Adjuvant Cytosolic Energy Replenishment in a Murine Model of Hemorrhagic Refractory Shock

Zakaria¹,

Joseph²,

Jehan³

et al. 2017

J Surg Anesth

View full text Add to dashboard Cite

Objective: A refractory haemorrhagic hypovolemic shock (HS) resuscitation is challenging. HS is associated with profound depletions of cellular energy nucleotides that can cause death from a cardio-circulatory arrest. To prevent an imminent cardio-circulatory arrest, vasopressors, commonly norepinephrine is usually temporarily administered to manage a persistent hypotension that is not corrected by aggressive resuscitation efforts. The objective of this study is to determine the post-resuscitation survival time after adjuvant resuscitations of a refractory HS with norepinephrine, vasopressin or direct cytosolic energy (adenosine-5`-triphosphate, ATP) replenishment using lipid vesicles encapsulating ATP (ATPv).Methods: 50 male Sprague-Dawley rats were randomized to 5 groups of 10 each: HS/conventional resuscitation (CR), HS/CR+Norepinephrine, HS/CR+Vasopressin, HS/CR+Vesicles, and HS/CR+ATPv. (HS=initial removal of 30% of the calculated blood volume, a 60 min hypotensive phase, and a subsequent transection of the spleen for uncontrolled haemorrhage until persistent shock index (SI)>5 and mean arterial pressure (MAP)<35 mmHg were achieved; CR=shed blood returned+double the shed blood volume as lactated Ringer's solution). Direct cytosolic ATP replenishment was accomplished with ATPv, which are highly fusogenic lipid vesicles encapsulating ATP. Fusion of the ATPv with the cell membrane on contact, allows for direct cytosolic ATP delivery. We determined the post-resuscitation survival time as the end-point of the study.Results: All animals displayed the same class of shock as demonstrated by the SI and MAP. Median postresuscitation survival times (computed by the Kaplan-Meier survival curves and the long-rank Mantel-Cox test) were as follows: HS/CR=35.5 min; HS/CR+Norepinephrine=38.5 min; HS/CR+Vasopressin=20 min; HS/CR+Lipid Vesicles control=88.5 min; and HS/CR+ATPv=158.5 min (p<0.001). Conclusion:The replenishment of the depleted cellular cytosolic energy stores in a refractory haemorrhagic hypotensive shock prolongs post-resuscitation survival time and delays cardio-circulatory arrest. This buys time for the initiation of definitive resuscitation protocols. Cellular energy failure appears to contribute to the pathogenesis of shock refractoriness to resuscitation efforts. The temporary administration of vasopressors for pressure-support resuscitation of a refractory haemorrhagic hypovolemic shock exerts no survival benefits.

show abstract

Section: Discussionmentioning

confidence: 99%

Improved Post-Resuscitation Survival Time with Adjuvant Cytosolic Energy Replenishment in a Murine Model of Hemorrhagic Refractory Shock

Zakaria¹,

Joseph²,

Jehan³

et al. 2017

J Surg Anesth

View full text Add to dashboard Cite

show abstract

“…Analysis of red blood system state throughout the annual cycle revealed periodic uctuations of some parameters. For example, hemoglobin concentration and the number of circulating RBCs showed monocyclic dynamics (Bhagat, Banerjee, 1986;Maslova et al, 1988;Joshi, 1989;Al-Hassan et al, 1990), which indirectly re ected a change in the balance between productive and destructive processes. This dynamic depends on water temperature uctuations.…”

Section: Introductionmentioning

confidence: 99%

Annual variation of red blood cell number and composition of erythroid cells in circulating blood and head kidney of golden grey mullet (Chelon auratus Risso, 1810)

Солдатов

Rychkova

Кухарева

et al. 2023

Preprint

View full text Add to dashboard Cite

The changes in the number of erythrocytes in the blood and the production of erythrocytes by the hematopoietic tissue of the golden grey mullet (Chelon auratus Risso, 1810) during the annual cycle were studied. Catching and delivery of fish to the aquarium was carried out monthly. The content of immature erythroid forms was determined: pronormoblasts, basophilic and polychromatophilic normoblasts, in the head kidney (pronephros) and circulating blood. It has been established that the processes of erythropoiesis in the hematopoietic tissue of the golden grey mullet proceed irregularly. The active production of erythroid mass is mainly confined to the post-spawning period. This is evidenced by an increase in the content of immature erythroid forms in the head kidney and blood. This coincides with a general increase in the number of red blood cells in the circulation system and indicates a shift in the erythrocyte balance in favor of production processes. In the rest of the time, the processes are opposite. It is assumed that this is due to the peculiarities of the organization of the red blood system, which excludes the regular production of erythropoietin in the kidneys.

show abstract

Changes of physiological and biochemical characteristics of rat erythrocytes after blood loss

Cited by 2 publications

References 5 publications

Improved Post-Resuscitation Survival Time with Adjuvant Cytosolic Energy Replenishment in a Murine Model of Hemorrhagic Refractory Shock

Improved Post-Resuscitation Survival Time with Adjuvant Cytosolic Energy Replenishment in a Murine Model of Hemorrhagic Refractory Shock

Annual variation of red blood cell number and composition of erythroid cells in circulating blood and head kidney of golden grey mullet (Chelon auratus Risso, 1810)

Contact Info

Product

Resources

About