“…According to the method of our previous study [ 6 , 11 ], immunohistochemical staining for CB was performed using rabbit anti-CB (1:200, Millipore, Temecula, CA, U.S.A.), biotinylated goat anti-rabbit IgG (1:200, Vector, Burlingame, CA, U.S.A.) and streptavidin peroxidase complex (1:200, Vector). In order to establish the specificity of the immunostaining, a negative control test was carried out with pre-immune serum instead of primary antibody.…”