Changes of structures and biosynthesis/hydrolysis-associated genes expression of glucans at different Volvariella volvacea maturity stages

Shao, Ze-Yu; Zan, Xinyi; Jia, Wei; Sun, Lei; Cui, Fengjie; Zhu, Hong-An; Sun, Wenjing; Liang, Yingying; Fu, Xin; Zhang, Jingsong; Zhao, Xuyao

doi:10.1016/j.ijbiomac.2021.09.128

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…The potential anomeric configuration and glycosidic linkage of the synthesized oligoglucoses or glucans by CMGLS were identified based on the 1 H and 13 C NMR spectroscopy. , As shown in Figure C,D, CMGLS-synthesized products had a typical β-glycosidic configuration with four signals in 1 H NMR spectrum with chemical shifts of δ 4.79, 4.76, 4.61 and 4.42 in the anomeric region (Figure C), which confirmed that the synthesized oligoglucoses or glucans contained β-configuration for glucopyranosyl residues. The 13 C NMR spectrum (Figure D) showed carbon peaks at 60.92, 68.45, 72.93, 76.44, 86.24, and 103.09 ppm for C6, C4, C2, C5, C3, and C1, respectively, which indicated the existence of 1,3 linkage since the resonance signal at 86.24 ppm is the typical peak for C3 involved in the glycosidic linkages of β-1,3-glucans …”

Section: Resultsmentioning

confidence: 56%

Functional Characterization and Structural Basis of the β-1,3-Glucan Synthase CMGLS from Mushroom Cordyceps militaris

Zan

Sun

et al. 2022

J. Agric. Food Chem.

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β-1,3-Glucan synthases play key roles in glucan synthesis, cell wall assembly, and growth of fungi. However, their multi-transmembrane domains (over 14 TMHs) and large molecular masses (over 100 kDa) significantly hamper understanding of their catalytic characteristics and mechanisms. In the present study, the 5841-bp gene CMGLS encoding the 221.7 kDa membranebound β-1,3-glucan synthase CMGLS in Cordyceps militaris was cloned, identified, and structurally analyzed. CMGLS was partially purified with a specific activity of 87.72 pmol/min/μg, a purification fold of 121, and a yield of 10.16% using a product-entrapment purification method. CMGLS showed a strict specificity to UDP-glucose with a K m value of 84.28 μM at pH 7.0 and synthesized β-1,3-glucan with a maximum degree of polymerization (DP) of 70. With the assistance of AlphaFold and molecular docking, the 3D structure of CMGLS and its binding features with substrate UDP-glucose were proposed for the first time to our knowledge. UDPglucose potentially bound to at least 11 residues via hydrogen bonds, π-stacking ,and salt bridges, and Arg 1436 was predicted as a key residue directly interacting with the moieties of glucose, phosphate, and the ribose ring on UDP-glucose. These findings would open an avenue to recognize and understand the glucan synthesis process and catalytic mechanism of β-1,3-glucan synthases in mushrooms.

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Section: Resultsmentioning

confidence: 56%

Functional Characterization and Structural Basis of the β-1,3-Glucan Synthase CMGLS from Mushroom Cordyceps militaris

Zan

Sun

et al. 2022

J. Agric. Food Chem.

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“…The variances in monosaccharide composition could be attributed to the different growth stages and batches of raw materials . Concretely, this may be related to the activity of the enzymes involved in sugar biosynthesis and hydrolysis at different stages . For example, Zhu el al .…”

Section: Resultsmentioning

confidence: 99%

“…36 Concretely, this may be related to the activity of the enzymes involved in sugar biosynthesis and hydrolysis at different stages. 18 For example, Zhu el al. 37 found that the higher activity of β-D-mannan enzyme resulted in the decrease of mannose more than that of galactose during fenugreek seeds germination.…”

Section: Dynamic Changes Of Alkali-extracted Polysaccharides From a C...mentioning

confidence: 99%

“…17 growth stages based on their respective morphologies. 18 Recent studies indicated that different growth stages can obviously affect yields and biological activities of mushrooms polysaccharides. For example, Cui et al 19 revealed that the yields of polysaccharides from Pleurotus eryngii fruiting body increased along the growth process, with the highest yield at the mature stage.…”

Section: Introductionmentioning

confidence: 99%

“…Their formations include vegetative hyphae aggregation, primordia differentiation, and fruiting bodies’ growth . Among them, the development of fruiting bodies can be divided to multiple growth stages based on their respective morphologies . Recent studies indicated that different growth stages can obviously affect yields and biological activities of mushrooms polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Changes of the Physicochemical Properties and Structural Characteristics of Alkali-Extracted Polysaccharides from Agrocybe cylindracea Across the Growth Process

Liu,

Chen,

Wang

et al. 2024

J. Agric. Food Chem.

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Polysaccharides derived from Agrocybe cylindracea have been demonstrated to exhibit various bioactivities. However, studies on their structural characteristics during the growth process are limited. This study aimed to compare the physicochemical properties and structural characteristics of alkali-extracted polysaccharides from A. cylindracea fruiting bodies (JACP) across four growth stages. Results showed that the extraction yields and protein levels of JACP declined along with the growth of A. cylindracea, while the contents of neutral sugar and glucose increased significantly. However, JACP exhibited structural characteristics similar to those across the four stages. Four polysaccharide subfractions were isolated from each growth stage, including JACP-Et30, JACP-Et50, JACP-Et60, and JACP-Et70. JACP-Et30 from the four stages and JACP-Et50 from the initial three stages were identified as heteroglucans with β-1,3-d-Glcp and β-1,6-d-Glcp residues as main chains, respectively. However, other subfractions were considered as β-1,6-d-glucans containing minor glucuronic acid. These subfractions were predominantly replaced by Glcp residues at the O-3 and O-6 positions. Overall, while JACP exhibited variable physicochemical properties, its structural characteristics remained stable during the growth process, offering new insights into its potential applications in the food and medicinal industries.

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Insights into dynamic evolution of glucuronofucogalactoglucan from water extract of Agrocybe cylindracea during maturation

Liu,

Chen,

Luo

et al. 2024

Carbohydrate Polymers

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Changes of structures and biosynthesis/hydrolysis-associated genes expression of glucans at different Volvariella volvacea maturity stages

Cited by 7 publications

References 37 publications

Functional Characterization and Structural Basis of the β-1,3-Glucan Synthase CMGLS from Mushroom Cordyceps militaris

Functional Characterization and Structural Basis of the β-1,3-Glucan Synthase CMGLS from Mushroom Cordyceps militaris

Changes of the Physicochemical Properties and Structural Characteristics of Alkali-Extracted Polysaccharides from Agrocybe cylindracea Across the Growth Process

Insights into dynamic evolution of glucuronofucogalactoglucan from water extract of Agrocybe cylindracea during maturation

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