Changes of the Laser-Induced Blue, Green and Red Fluorescence Signatures during Greening of Etiolated Leaves of Wheat

Stober, F.; Lichtenthaler, Hartmut K.

doi:10.1016/s0176-1617(11)81022-9

Cited by 58 publications

(28 citation statements)

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“…3a). Therefore, the peak maximum is shifted to longer wavelengths compared to the results obtained for the spermatophytes, where the main emission band is typically in the ranges of 430-450 nm and 520-530 nm (Stober and Lichtenthaler, 1992;Lichtenthaler and Schweiger, 1998). In P. bifurcatum only a shoulder of the peak was observed in the blue range spectrum, however, changes in the course of fluorescence curves may vary depending on the absorption characteristics of the leaves (Stober and Lichtertahler, 1993;Gitelson et al, 1998).…”

Section: Blue-green and Red Leaf Fluorescencementioning

confidence: 74%

A Low Ratio of Red/Far-Red in the Light Spectrum Accelerates Senescence in Nest Leaves of Platycerium Bifurcatum

Oliwa¹,

Kornaś²,

Skoczowski³

2017

Acta Biologica Cracoviensia S. Botanica

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The fern Platycerium bifurcatum is a valuable component of the flora of tropical forests, where degradation of local ecosystems and changes in lighting conditions occur due to the increasing anthropogenic pressure. In ferns, phytochrome mechanism responsible for the response to changes in the value of R/FR differs from the mechanism observed in spermatophytes. This study analyzed the course of ontogenesis of nest leaves in P. bifurcatum at two values of the R/FR ratio, corresponding to shadow conditions (low R/FR) and intense insolation (high R/FR). The work used only non-destructive research analysis, such as measurements of reflectance of radiation from the leaves, their blue-green and red fluorescence, and the chlorophyll a fluorescence kinetics. This allowed tracing the development and aging processes in the same leaves. Nest leaves are characterized by short, intense growth and rapid senescence. The study identified four stages of development of the studied leaves related to morphological and anatomical structure and changing photochemical efficiency of PSII. Under the high R/FR ratio, the rate of ontogenesis of the leaf lamina was much slower than under the low R/FR value. As shown, the rapid aging of the leaves was correlated with faster decline of the chlorophyll content. It was shown that leaf senescence was accompanied by accumulation of polyphenols, anthocyanins and carotenoids on the basis of reflectance and fluorescence measurements in the blue-green range.Keywords: chlorophyll a fluorescence; fern; nest leaves; photomorphogenesis; R/FR ratio; reflectance Abbreviations:Area -surface area above the chlorophyll fluorescence induction curve; ARI -anthocyanin reflectance index; BRI -browning reflectance index; CRI 1 -carotenoid reflectance index; DI 0 /RC -total energy dissipation not trapped by the PSII reaction center; ET 0 /RC -rate of electron transfer by the active PSII reaction center; F v /F 0 -maximum efficiency of the water-splitting reaction of the donor side of PSII; F v /F m -maximum quantum yield of PS II; H -R/FR higher value of the red/far red ratio; L-R/FR -lower value of the red/far red ratio; PAR -photosynthetically active radiation; PI -PSII vitality index; PSII -photosystem II; Q A -plastochinon Q A ; RC -reaction center; RC/ABS -reaction center density ratio in terms of chlorophyll; R/FR -photon intensity between 660-670 nm/photon intensity between 725-735 nm; TR 0 /RC -energy trapping of one active reaction center; WBI -water band index. * Corresponding authors,

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Section: Blue-green and Red Leaf Fluorescencementioning

confidence: 74%

A Low Ratio of Red/Far-Red in the Light Spectrum Accelerates Senescence in Nest Leaves of Platycerium Bifurcatum

Oliwa¹,

Kornaś²,

Skoczowski³

2017

Acta Biologica Cracoviensia S. Botanica

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“…2 e 3), indicando que houve perturbação no processo fotossintético. Em condições de estresse a taxa de fluorescência (FR) das bandas de emissão da clorofila no vermelho e vermelho distante, denotadas pela relação F690/F735, é aumentada (SCHREIBER, 1983;STOBER;LICHTENTHALER, 1992). O aumento dessa relação F690/F735 indica que a capacidade de captação da luz é superior à capacidade de aproveitamento fotoquímico, resultando na emissão de fluorescência.…”

Section: Resultsunclassified

Estresse salino em duas variedades de cana-de-açúcar: enzimas do sistema antioxidativo e fluorescência da clorofila

Willadino¹,

Filho²,

Silva³

et al. 2011

Rev. Ciênc. Agron.

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Resumo -O Brasil destaca-se por ser o maior produtor de cana-de-açúcar (Saccharum officinarum L.) e por dominar todos os estágios da tecnologia de produção de açúcar e etanol. Atualmente se observa a expansão dessa cultura para a região semiárida brasileira, a qual apresenta solos propensos à salinização. O presente trabalho objetivou avaliar a resposta à salinidade de duas variedades de cana-de-açúcar. O ensaio foi implantado em casa de vegetação, em delineamento inteiramente casualizado, em esquema fatorial 2 x 8 com cinco repetições, correspondendo a duas variedades de cana-de-açúcar (RB867515 e RB863129) e oito níveis de NaCl (0; 25; 50; 75; 100; 125; 150; 200 mM de NaCl). A salinidade induziu aumento na fluorescência e diminuição na concentração de clorofila em ambas as variedades. A redução da clorofila, entretanto, foi maior na variedade RB867515. Observou-se, nesta mesma variedade, aumento da atividade da peroxidase do ascorbato (APX) e da catalase (CAT), enquanto na variedade RB863129 ocorreu redução na atividade dessas enzimas. Esses resultados indicam que a variedade RB867515 apresenta um mecanismo mais eficiente na proteção da planta contra danos no aparato fotoquímico e contra a ação de espécies reativas de oxigênio, em particular o H 2 O 2 .Palavras-chave -Catalase. Fluorescência da clorofila. Peroxidase. Saccharum officinarum.Abstract -Brazil is seen as being the largest producer of sugarcane (Saccharum officinarum L.) and of mastering all stages in the production of sugar and ethanol. Currently this production is being expanded into the semi-arid regions of Brazil, where there are soil-types prone to salinity. This study aimed to evaluate the response to salinity in two sugarcane genotypes, RB867515 and RB863129. Salinity caused an increase in chlorophyll fluorescence and a reduction of chlorophyll concentration in both varieties. The reduction of chlorophyll however was higher in RB867515. In this same type, an increase in the activity of ascorbate peroxidase (APX) and catalase (CAT) was observed, while in genotype RB863129 there was a reduction in the activity of these two enzymes. These results show that RB867515 has a mechanism which better protects the plant against damage to the photochemical system and the action of reactive oxygen species, particularly H 2 O 2 .

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“…For instance, an increase in green fluorescence has been reported in pine needles as a result of exposure to pollutants (Schneckenburger and Frenz, 1986). An increased BGF was also recorded in senescent soybean leaves (Chappelle et al, 1984b), senescent (Stober and Lichtenthaler, 1992). The common feature of these reports was a reduced amount of Chl per unit of leaf area.…”

mentioning

confidence: 79%

“…beech leaves , and etiolated wheat leaves (Stober and Lichtenthaler, 1992). The common feature of these reports was a reduced amount of Chl per unit of leaf area.…”

mentioning

confidence: 99%

Characterization of Blue-Green Fluorescence in the Mesophyll of Sugar Beet (Beta vulgaris L.) Leaves Affected by Iron Deficiency

1994

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The mesophyll of sugar beet (Beta vulgaris 1.) leaves emits red (chlorophyll a ) fluorescence and blue-green fluorescence when excited with ultraviolet light. The intensity of blue-green fluorescence was increased in mesophylls affected by iron deficiency. This increase was large and progressive. It was concomitant with a decrease of photosynthetic pigments per unit of leaf area. Most of the increase in blue-green fluorescence can be explained by the decrease of the screening of ultraviolet light by chlorophylls and carotenoids. In addition, chlorophylls selectively reabsorb blue fluorescence, which leads to a change in the form of the fluorescence emission spectra. This effect induces an increase of the blueto-green fluorescence ratio in control mesophylls that was concomitant with the decrease of chlorophyll per unit of leaf area. Iron deficiency induced a decrease of the blue-to-green fluorescence ratio that may be attributed to an accumulation of flavins fluorescing in the green. Time-resolved fluorescence measurements indicate that they are mostly riboflavin and/or flavin mononucleotide phosphate. Our data also indicate that the blue-green fluorescence emitted from the mesophyll contains fluorescence of nicotinamide nucleotides.In the last decade several spectroscopic methods have been considered for the characterization of the physiological state of plants. Among them, the use of the fluorescence signal emitted by plants has been the object of intense research activity. Measurements of red (Chl a ) fluorescence have become a useful investigational tool in photosynthesis research, plant physiology, and early detection of stress conditions in plants ( Weis, 1984, 1991). Red fluorescence is usually excited in the visible part of the spectrum. Upon excitation with UV light, leaves also emit blue (maximum at 450 nm) and green (shoulder at 530 nm) fluorescence (Chappelle et al., 1984b(Chappelle et al., , 1991Goulas et al., 1990;Lang et al., , 1992. The intensity and form of the emission spectra of the UV light-induced fluorescence are influenced by environmental conditions (Chappelle et al., 1984a(Chappelle et al., , 1984bTheisen, 1988), or by stage of development. For instance, an increase in green fluorescence has been reported in pine needles as a result of exposure to pollutants (Schneckenburger and Frenz, 1986). An increased BGF was also recorded in senescent soybean leaves (Chappelle et al., 1984b), senescent (Stober and Lichtenthaler, 1992). The common feature of these reports was a reduced amount of Chl per unit of leaf area. This is also the most obvious characteristic of leaves affected by iron deficiency (Bolle-Jones and Notton, 1953;Terry, 1980; Abadia et al., 1989;Morales et al., 1990). Therefore, we used iron deficiency as a model system to investigate the effect of Chl on the BGF.Chl's and carotenoids absorb UV light (Lichtenthaler, 1987;Chappelle et al., 1990Chappelle et al., , 1991. This absorption attenuates the UV light that reaches the fluorophores fluorescing bluegreen. The result is a si...

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Changes of the Laser-Induced Blue, Green and Red Fluorescence Signatures during Greening of Etiolated Leaves of Wheat

Cited by 58 publications

References 14 publications

A Low Ratio of Red/Far-Red in the Light Spectrum Accelerates Senescence in Nest Leaves of Platycerium Bifurcatum

A Low Ratio of Red/Far-Red in the Light Spectrum Accelerates Senescence in Nest Leaves of Platycerium Bifurcatum

Estresse salino em duas variedades de cana-de-açúcar: enzimas do sistema antioxidativo e fluorescência da clorofila

Characterization of Blue-Green Fluorescence in the Mesophyll of Sugar Beet (Beta vulgaris L.) Leaves Affected by Iron Deficiency

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