Changes of Tyrosine Phosphorylation in Liver and Kidney of Polycystic Ovarian Rats Induced by Letrozole

Abstract

is a drug commonly used for breast cancer treatment since it can decrease estrogen level. In experimental animal, the Letro has been used to induce the polycystic ovarian syndrome (PCOS) model. Tyrosine phosphorylation (TyrPho) is an essential process in various biological functions both normal and abnormal conditions especially reproduction. Although some side effects of Letro are reported, the alterations of TyrPho responsible for liver and kidney functions have never been demonstrated. In this study, the blood serum, liver, and kidney of control and PCOS rats induced with Letro (orally, 1 mg/ KgBW) for consecutive 21 days were used to determine the serum biochemical components and to investigate the TyrPho expression using western blot analysis. Histopathology of such tissues was observed by Masson's trichrome staining. The results showed that Letro did not affect histological structures but significantly increased the serum levels of urea nitrogen, cholesterol, triglyceride, HDL, LDL, ALT, AST, and alkaline phosphatase. Additionally, the TyrPho protein expressions of 32 and 27 kDas in liver and of 55 and 43 kDas in kidney were increased while of a kidney 26 kDa was decreased as compared to those of control. In conclusion, this recent study indicated that the changes of TyrPho proteins in liver and kidney induced with Letro associated with their functions by alteration of serum biochemical levels.

Erythropoietin, Myoinositol and Metformin Modulate Insulin Sensitivity Indices, Pancreatic Beta Cell Mass and Hepatocellular Changes in a Rat Model of Polycystic Ovary Syndrome

“…Dilated sinusoids and neutrophilic infiltration were observed. Similar results were previously reported by Chaiyamoon et al (31) . In addition, in a previous study on androgen Induced model of Polycystic Ovary, the appearance of necrotic foci were observed in the liver histology in addition to the inflammatory infiltration and dilated sinusoids as seen in our results.…”

Alterations of serum biochemical parameters and tyrosine phosphorylation in kidney and liver of chronic stress-induced rats

“…To gain our knowledge, CS also changed the functional proteins in liver and kidney especially TyrPho and Hsp 70 proteins. liver and kidney which similar to that stress caused by drug effect called "letrozol" as demonstrated previously (Chaiyamoon et al, 2020). In contrast, a previous study has revealed that the glomerular volume of kidney in CS animals was significantly decreased (Marchon et al, 2018).…”

“…In normal condition, TyrPho also has been observed in the rat kidney (Zhang et al, 2008). Moreover, it was documented that the expression of TyrPho proteins in the liver and kidney was totally changed with other functional parameters in stress rats with polycystic ovarian (Chaiyamoon et al, 2020). For chronic stress model in this study, we suggested that TyrPho proteins also play the essential roles for liver and kidney functions although the individual protein need to be further identified and characterized to elucidate the actual mechanism.…”

“…Such glomerular volume loss in stress condition was shown to associate with hypertension (Hoy et al, 2008). Currently, Chaiyamoon et al (2020) have reported not only the changes of functional parameters of liver and kidney, but also alterations of the tyrosine phosphorylation in polycystic ovary syndrome, a stress condition of women. However, such changes especially expressions of tyrosine phosphorylated proteins in liver and kidney of male have never been elucidated.…”