In this study, secular trends in anthropometric characteristics, physical fitness, physical activity, and biological maturity over the past 25-35 years in Flemish adolescents were investigated. Representative cross-sectional samples of 12-18-year-old secondary school children (11,899 assessments in boys in 1969-1974, 4,899 girls in 1979-1980, 1,429 boys and 1,772 girls in 2005) and parent-offspring pairs tested at approximately the same age during adolescence (55 father-son pairs, mean age fathers = 15.47 years, mean age sons = 15.38 years; 62 mother-daughter pairs, mean age mothers = 16.63 years, mean age daughters = 15.01 years) were used. The cross-sectional data were analyzed in 6 yearly age-categories using Wilcoxon rank sum tests. For the parent-offspring data paired t-tests, simple linear regressions to adjust for parent-offspring differences in chronological age and multiple linear regressions to adjust for parent-offspring differences in chronological and skeletal age were conducted. The cross-sectional study generally revealed an increase in weight, stature, BMI, skinfolds and trunk-extremity index, and a decrease in the performance on several physical fitness tests. In the parent-offspring study, only sons were maturationally advanced compared to fathers. Even after adjustment for parent-offspring differences in chronological age and in chronological and skeletal age, results for stature, trunk-extremity index and physical fitness were generally similar to the cross-sectional study. No secular trend was observed for sports participation. The fact that the positive secular trends in weight, BMI, and skinfolds of the cross-sectional study were not entirely confirmed in the parent-offspring study is probably due to higher similarity in genetic and familial background, higher socio-economic status, and more health-consciousness of the latter.