Changing faecal population of escherichia coli in hospital medical patients

Em, Cooke; Ewins, S. P.; Ra, Shooter

doi:10.1136/bmj.4.5683.593

Cited by 66 publications

(33 citation statements)

References 6 publications

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“…Nevertheless, one cannot exclude the possibility of hospital infection caused by strains of B. fragilis prevalent in the various hospitals. Patients could acquire new hospital strains, similar to Escherichia coli (Cooke, Ewins & Shooter, 1969) and to Pseudomonas aeruginosa (Al-Dujaili & Harris, 1975 …”

Section: Discussionmentioning

confidence: 99%

The distribution ofBacteroides fragilisserotypes amongst clinical strains

Elhag

Tabaqchali

1978

J. Hyg.

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Three hundred and twenty-two strains ofBacteroidesfragilis isolatedfrom infected patients at three different hospitals were tested against 20 type specific B. fragilis antisera using the tube agglutination technique. Of these strains 41'3 % were assigned to a single 0-serotype, a further 20-5 % were agglutinated by several antisera and could not be classified and the remainder showed no reactions. Three different serotypes were prevalent in the three hospitals and minor geographical variation was observed.No correlation was found between serotypes and the origin of infection, but those from the blood were the most readily typable strains. No correlation was found between serotypes and biotypes of B. fragilis.

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Section: Discussionmentioning

confidence: 99%

The distribution ofBacteroides fragilisserotypes amongst clinical strains

Elhag

Tabaqchali

1978

J. Hyg.

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“…On two occasions the ingested strain was not made antibiotic resistant and was identified in one case by slide agglutination using OK antiserum prepared against the organism, and in the other by typing ten colonies from the MacConkey medium with 0 antisera. Five colonies of each colonial type of coliform appearing on the MacConkey plate, which contained no antibiotic, were confirmed as Escherichia and serotyped with 0 antisera 1-25, 39, and 75 as described previously (Cooke et al, 1969). Strains not agglutinated by these antisera are referred to as " untypable ".…”

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confidence: 99%

Fate Of Ingested Escherichia Coli In Normal Persons

Cooke

Hettiaratchy

Buck

1972

Journal of Medical Microbiology

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THE factors affecting strains of Escherichia coli carried in the bowel of man are poorly understood. Some workers have found the coliform population of the bowel to be relatively stable (Sears, Brownlee and Uchiyama, 1950), but others have shown that the faecal coliform flora may change (Cooke, Ewins and Shooter, 1969; Wiedmann and Knothe, 1969) and this, in one instance, has been related to the ingestion of large numbers of E. coli in food . It has also been postulated that these strains of E. coli may be of animal origin, and may reach the prepared food by cross-contamination in the kitchen .It is not known whether all strains of ingested E. coli will establish themselves in the bowel, what numbers if ingested will be detectable in the faeces, and whether strains from different sources or of different serotype or colicine type behave differently. The experiments reported here were performed in an attempt to answer these questions. MATERIALS AND METHODSVolunteers. These were one man and two women who were members of the laboratory staff. They were aged 33-37 yr, and had no known abnormality of the gastro-intestinal tract. They were not receiving antibiotics and were eating a normal mixed diet. The food eaten by these volunteers was not examined for the presence of coliform bacteria.E. coli strains for ingestion. Twelve strains of E. coli, some of human and some of animal origin, were ingested. The sources, serotype, antibiotic sensitivity, colicinogenicity, and colicine sensitivity of the strains are shown in table I.The human strains were isolates from normal faeces of patients in a medical ward. The four animal strains were faecal isolates from healthy animals.Except on two occasions, the strains were rendered resistant to streptomycin or nalidixic acid in the laboratory before they were ingested. From a 5-hr broth culture of the test strain 0.5 ml was added to 200 ml of cold milk which was drunk in the late afternoon, 3-4 hr after the last meal. The numbers of organisms ingested, determined by surface-viable counts on blood agar, ranged from 105 to 108 except that on one occasion 1011 freeze-dried organisms were ingested. Examination of faeces. Specimens of faeces were examined on at least three occasions before ingestion of E. coli, and on three occasions after its disappearance. In each experiment, every specimen of faeces passed was examined.Specimens were examined as soon as they were passed or were stored for up to 24 hr at

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“…Não nos preocupamos com a identificação mais específica dos microrganismos isolados, a não ser com o estafilococo, porquanto, através de numerosos trabalhos (Kennedy e col. 21 , Spencer e col. 46 , Cooke e col. 8 , Shooter e col. 44,45 , Weil e col. 53 , Dans e col. 9 , Falcão e col.…”

Section: Antibiogramaunclassified

Infecções hospitalares no município de Araraquara, SP (Brasil)

et al. 1976

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Foi demonstrada a etiologia das infecções adquiridas em hospitais da cidade de Araraquara, S. Paulo, Brasil, verificando-se o comportamento das bactérias isoladas frente a 10 antimicrobianos. A metodologia bacteriológica seguida conduziu à especiação bacteriana, com exceção do estafilococos que foi identificado através da fagotipagem. Dos 171 processos infecciosos, adquiridos nos hospitais no período de setembro de 1974 a maio de 1975, foram isolados 211 microrganismos, sendo 73,0% de bactérias Gram negativas e 27,0% de bactérias Gram positivas. Verificou-se um alto nível de resistência à maioria dos antibióticos. As bactérias Gram negativas apresentaram menor resistência à Gentamicina (22,2%), enquanto as Gram positivas apresentaram menor resistência à Cefalotina (17,7%), à Fosfomicina (25,5%) e à Gentamicina (29,4%). Os resultados permitiram antever sérias dificuldades quanto à ação dos antimicrobianos sobre as bactérias, assim como a intensa disseminação das bactérias Gram negativas, especialmente a Escherichia coli e as Pseudomonas.

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Changing faecal population of escherichia coli in hospital medical patients

Cited by 66 publications

References 6 publications

The distribution ofBacteroides fragilisserotypes amongst clinical strains

The distribution ofBacteroides fragilisserotypes amongst clinical strains

Fate Of Ingested Escherichia Coli In Normal Persons

Infecções hospitalares no município de Araraquara, SP (Brasil)

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