Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study

Abstract

Background: Postmortem interval (PMI) is an important in forensic practice. Many studies in blood and tissue of animals have revealed correlation between adenosine triphosphate (ATP) level and PMI. In this study aimed to explore the correlation between human blood ATP and its metabolites level and PMI in vitro model. Methods:Ten milliliter of venous blood samples were collected form four healthy men and contained in EDTA tube. One milliliter of the blood sample was divided at 0, 1, 2, 4, 8, 12 and 24 h, respectively. The blood samples were extracted and measured ATP, adenosine diphosphate (ADP) and adenosine monophosphate (AMP) level by High performance liquid chromatography-diode array. Energy charge value was calculated to predict charge of ATP metabolites. Results:The blood ATP, ADP and AMP levels increased in the first hour. The blood ATP, ADP and AMP levels did not obviously change before 12 hour. The blood ATP level trended to decrease but the blood ADP and AMP levels trended to increase after 24 hour. Energy Charge was not change in first 12 hour but trended to decrease after 24 hour. Conclusion:The blood ATP level and its metabolites changed after 12 hour and apply for the PMI investigation.

Postmortem Alteration of Purine Metabolism in Coronary Artery Disease

“…The levels of ATP and GTP in endocardial cells of CAD patients associated with left ventricular hypertrophy were decreased [41]. ATP and its metabolites showed a decreased tendency after death [42]. These two phenomena might appear in CAD cadavers.…”