Changing responsiveness of luteal cells of the marmoset monkey (Callithrix jacchus) to luteotrophic and luteolytic agents during normal and conception cycles

Webley, G. E.; Richardson, M. C.; Summers, P.M.; Given, A.; Hearn, Jack

doi:10.1530/jrf.0.0870301

Cited by 21 publications

(9 citation statements)

References 16 publications

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“…A significant rise in serum gonadotrophin activity [measured by mouse Leydig cell activity, which reacts to both luteinizing hormone (LH) and CG] does not occur until the 17th day of pregnancy (Hearn et al, 1988). However, corpora lutea removed from the marmoset on the 14th day of pregnancy secreted more progesterone in culture than those from nonpregnant animals (Webley et al, 1989). Furthermore, Webley etal.…”

Section: Discussionmentioning

confidence: 95%

Lack of evidence for the prodection of interreron -α-like species by the cultured human pre-embryo

Gunn¹,

Homa

Searle³

et al. 1994

Human Reproduction

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The aim of this study was to determine if the human pre-embryo produces a substance similar to the trophoblast interferon secreted by ruminant trophoblasts. Human embryos surplus to in-vitro fertilization (IVF) treatment were cultured up to 14 days following IVF. Viable cultures were determined by microscopic examination and by assay of the culture medium for human chorionic gonadotrophin (HCG). Four stages of development were visually identified: pre-blastocyst, unhatched, part-hatched and fully hatched blastocyst. HCG was detected in medium which had contained the more developmentally advanced embryos. A total of 62 samples were assayed for human interferon-alpha (IFN-alpha), including all cultures presumed viable. None contained detectable IFN-alpha immunoreactivity. Out of 14 candidate samples subjected to cytopathic effect reduction assay, none contained antiviral activity. We suggest that a trophoblast-derived interferon, unlike HCG, does not play a significant role in the maternal recognition of pregnancy in humans.

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Section: Discussionmentioning

confidence: 95%

Lack of evidence for the prodection of interreron -α-like species by the cultured human pre-embryo

Gunn¹,

Homa

Searle³

et al. 1994

Human Reproduction

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“…The luteotrophic actions of PGF 2· appear to be luteal phase [2,16,17] and concentration-dependent [7]. These actions are reported to be strongest in the mid-luteal phase and during pregnancy of investigated species [11,[24][25][26]. Maximal luteotrophic responses to PGF 2· are reported at a concentration of PGF 2· which corresponds to the reported K d [12,13] of the PGF 2· receptor (approximately 1 nM) [7].…”

Section: Discussionmentioning

confidence: 99%

Interaction of Prostaglandin F<sub>2α</sub> and Prostaglandin-E<sub>2</sub> on Progesterone Production in Human Granulosa-Luteal Cells

Väänänen¹,

Tong²,

Väänänen³

et al. 2001

Neurosignals

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This study examined the effects of prostaglandin-F_2α (PGF_2α), prostaglandin-E₂ (PGE₂) and their interactions on progesterone production in human granulosa-luteal cells (GLCs). Human GLCs collected from in vitro fertilization patients were cultured for 1 (D₁) or 8 days (D₈), followed by a 24-hour treatment period, after which media were collected and radioimmunoassayed for progesterone. Seven-point PGF_2α and PGE₂ concentration-response curves were crossed into a matrix of 49 separate treatments. Responses were plotted in three dimensions and as two-dimensional ‘slices’. In D₁ cultured human GLCs neither PGF_2α nor PGE₂ alone had any effect on progesterone production, however two different combinations of these hormones led to at least a 3-fold increase in progesterone production. This stimulation was seen when cells were treated with 10^–6M PGF_2α plus 10^–9M PGE₂, and when they were treated with 10^–10M PGF_2α plus 10^–9M PGE₂. In D₈ GLCs, PGF_2α stimulated progesterone production maximally at 10^–9M, while the lowest (10^–11 M) and highest concentrations (10^–6 M) tested were ineffectual. On the contrary, in the presence of high concentrations of PGE₂ (10^–6 to 10^–7 M), PGF_2α-mediated stimulation of progesterone production was attenuated. In a similar fashion to PGF_2α, PGE₂ also acted in a luteotrophic manner, although the maximal stimulation of progesterone production was seen at a higher concentration (10^–8 to 10^–7 M). Likewise, PGE₂-mediated progesterone production was attenuated by the presence of high concentrations of PGF_2α (10^–6 to 10^–7 M). In conclusion, in D₁ human GLCs neither PGF_2α nor PGE₂ alone were luteotrophic, although specific combinations of these hormones were. Conversely, in D₈ GLCs both PGF_2α and PGE₂ stimulated progesterone production in a biphasic manner, while the presence of a high concentration of either of these prostaglandins attenuated the luteotrophic effects of the other. Therefore, PGF_2α and PGE₂ interacted in a concentration-dependent manner, resulting in a multimodal progesterone response, which was easily visualized using three-dimensional plots.

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“…injection containing a mixture of ketamine (Parke Davis, USA; 5 mg/350 g body weight) and xylazine (Rompun, Bayer; 1 mg/350 g body weight), and narcotized with a halothane-nitrous oxideoxygen mixture (Baxter, Unterschleißheim, Germany). Laparotomy was performed, and the tissue was removed by luteectomy as described by Webley et al (1989). The CLs were staged retrospectively to the different luteal phases by plasma P 4 determination twice per week after ovulation, and the ensuing analysis of the plasma P 4 on the day of luteectomy.…”

Section: Tissue Collectionmentioning

confidence: 99%

Luteotrophic effects of relaxin, chorionic gonadotrophin and FSH in common marmoset monkeys (Callithrix jacchus)

Beindorff¹,

Einspanier²

2010

Reproduction

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In early pregnant primates, relaxin (RLX) is highly upregulated within the corpus luteum (CL), suggesting that RLX may have an important role in the implantation of the blastocyst. Therefore, the aim of the present study was to investigate the local effects of RLX and gonadotrophins on the maintenance of the CL using an in vitro microdialysis system. CLs of common marmoset monkeys were collected by luteectomy during different stages of the luteal phase and early pregnancy. Each CL was perfused with either Ringer's solution alone or Ringer's solution supplemented with either porcine RLX (250, 500 and 1000 ng/ml) or gonadotrophins (50 IU/ml). Application of RLX provoked a significant luteal response of progesterone (P 4 ) and oestradiol (E 2 ) secretions during the mid-luteal phase (500 ng/ml: P 4 54G42%, E 2 24G11%; 1000 ng/ml: E 2 16G13%), and especially during the late luteal phase (250 ng/ml: P 4 53G10%; 500 ng/ml: P 4 44G15%; 1000 ng/ml: P 4 62G15%, E 2 18G7%). The effects of RLX on steroid secretion were irrespective of the RLX dosages. While treatment with human chorionic gonadotrophin did not affect luteal steroid or RLX secretion, the application of FSH resulted in a significant increase in the secretion of both P 4 (20G8%) and E 2 (37G28%), and a prominent rise in RLX during early pregnancy. In conclusion, our results demonstrate that RLX and FSH have a luteotrophic function in the marmoset monkeys; moreover, FSH has a function beyond its traditional role just as a follicle-stimulating hormone.Reproduction (2010) 139 923-930

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Changing responsiveness of luteal cells of the marmoset monkey (Callithrix jacchus) to luteotrophic and luteolytic agents during normal and conception cycles

Cited by 21 publications

References 16 publications

Lack of evidence for the prodection of interreron -α-like species by the cultured human pre-embryo

Lack of evidence for the prodection of interreron -α-like species by the cultured human pre-embryo

Interaction of Prostaglandin F<sub>2α</sub> and Prostaglandin-E<sub>2</sub> on Progesterone Production in Human Granulosa-Luteal Cells

Luteotrophic effects of relaxin, chorionic gonadotrophin and FSH in common marmoset monkeys (Callithrix jacchus)

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