Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil

Abstract

Phylogenetic and taxonomic characterization was performed for bacterium RB-25 T , which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurred at 20-37 C at pH 5-8 but not in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C 16:0 , C 18:1 !7c and summed feature 3 (C 16:1 !7c and/or iso-C 15:0 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed the presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 mol% phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25 T formed a distinct lineage within the family Enterobacteriaceae of the class Gammaproteobacteria. It exhibited a low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pantoea rwandensis LMG 26275 T (96.6 %), Rahnella aquatilis CIP 78.65 T (96.5 %), Pectobacterium betavasculorum ATCC 43762 T (96.4 %), Pantoea rodasii LMG 26273 T (96.3 %), Gibbsiella dentisursi NUM 1720 T (96.3 %) and Serratia glossinae C1 T (96.2 %). Multilocus sequence analyses based on fusA, pyrG, rplB, rpoB and sucA sequences showed a clear distinction of strain RB-25 T from the most closely related genera. Isolate RB-25 T could also be distinguished from members of these genera by a combination of the DNA G+C content, respiratory quinone system, fatty acid profile, polar lipid composition and other phenotypic features. Strain RB-25 T represents a novel species of a new genus, for which the name Chania multitudinisentens gen. nov., sp. nov. is proposed. The type strain is RB-25 T (=DSM 28811 T =LMG 28304 T ).

Scandinavium goeteborgense gen. nov., sp. nov., a New Member of the Family Enterobacteriaceae Isolated From a Wound Infection, Carries a Novel Quinolone Resistance Gene Variant

“…We tried to counteract this limitation by finding two additional strains belonging to the proposed novel taxon, by screening publicly available genome sequences. Even though this is not optimal from a taxonomic perspective, novel genera in family Enterobacteriaceae including Chania and Nissabacter (proposed reclassification to family Yersiniaceae) have previously been established from single strain investigations (Ee et al, 2016; Mlaga et al, 2017). In any case, our results confirm that strain CCUG 66741 T represents a novel taxonomic position within the family Enterobacteriaceae , representing a novel genus and species, for which the name Scandinavium goeteborgense gen. nov., sp.…”

Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25T gen. nov., sp. nov.

“…Isolated in a soil sample collected from a former municipal landfill site, C. multitudinisentens RB-25 T was initially misidentified as a member of the Serratia genus (Ee et al, 2014a). Further in-depth investigation later reclassified C. multitudinisentens RB-25 T as a novel genus (Ee et al, 2016). To date, C. multitudinisentens RB-25 T is only characterized for its quorum sensing properties (the production of C4-HSL, C6-HSL, and 3-oxo-C6-HSL) and its potential chitinolytic activity (Ee et al, 2014a; Lim et al, 2015b).…”

Enterobacteria in the 21st century: a review focused on taxonomic changes

“…The genera Coetzeea and Thorsellia, are the only genera described for the Thorselliaceae family. Even though this review focuses only on genera within the family Enterobacteriaceae described during the 21th century, Table 1 shows all the families and genera that belong to the order Enterobacteriales [13][14][15][16][17][18][19][20][21] , in order to show the genera related to Enterobacteriaceae.…”