Chaperones in the Parotid Gland: Localization of Heat Shock Proteins in Human Adult Salivary Glands

Abstract

Heat shock proteins (HSPs) are expressed or increased in response to various biological stresses. Moreover, these ‘stress proteins’ seem to be expressed by some cells living in physiological conditions. From then on, they could play an important physiological role in normal cell functioning. The best-known physiological role of these HSP proteins is to act as ‘molecular chaperones’. In this context, we have investigated the immunohistochemical expression of HSP27, HSP70, HSP90 and HSP110 in 10 human adult salivary glands. To highlight the presence of RNAm encoding HSP70, an in situ hybridization was performed. In our material, HSP27 was strongly expressed in the cytoplasm of striated duct cells and in some myoepithelial cells. The same localization was less stained for HSP70 and HSP90. The immunocytochemical reaction was weak or negative for HSP110 in striated ducts. HSPs were not expressed in acinic cells. In situ hybridization gave a positive signal in striated ducts with a probe encoding HSP70. Epithelial cells of the striated ducts and myoepithelial cells expressed HSP27, HSP70 and HSP90. These HSPs probably act in part as molecular chaperones for protein synthesis, transport and for several interactions between HSPs and different proteins.

Potential immunological functions of salivary Hsp70 in mucosal and periodontal defense mechanisms

“…albumin, blood group antigens, etc.). A passive transport or a small-capacity active transport [26,27] from the striated duct cells of human salivary glands is also possible, since a higher amount of Hsp70 was detected in these cells compared with others with immunocytochemical methods [82].…”

An Immunohistochemical Study of the Localization of Inducible Nitric Oxide Synthase (iNOS) and Heat Shock Protein (HSP) in Pleomorphic Adenoma

“…Although the localization of iNOS and the HSPss and their roles in normal salivary gland are well documented [1,5,10,21,31], little information is available on their role in neoplasm of salivary gland [4,5]. The present study showed iNOS and the HSPs are widely localized at neoplastic epithelium in pleomorphic adenoma.…”

“…The sections were rinsed in PBS and incubated with streptavidin-biotin peroxidase complex (SAB-PO kit; Histofine, Nichirei, Tokyo, Japan) according to manufacturer procedure, reacted with 3,3'-diaminobenzidine (DAB) and hydrogen peroxide for 5 min at room temperature, rinsed in distilled water, dehydrated, and mounted in Malinol. As a positive control, the staining in the normal salivary glands adjacent to the tumors was used with reference to previous reports [5,31]. All individuals were informed about the purpose of the study and consented to the study.…”

“…E-mail: yoshi-ab@hoku-iryo-u.ac.jp gland have been well documented [1,5,10,21,31], little information is available on them in the pleomorphic adenoma. The present study investigates the localization of iNOS and HSPs in pleomorphic adenoma.…”

Salivary extracellular heat shock protein 70 (eHSP70) levels increase after 59 min of intense exercise and correlate with resting salivary secretory immunoglobulin A (SIgA) levels at rest

“…Further research is required to identify the influence of the autonomic nervous system on eHSP70 secretion. Vanmuylder et al (2000) reported that eHSP70 is strongly expressed in epithelial cells of the striated ducts and weakly expressed in the acinar cells of the salivary glands. Therefore, it can be inferred that constitutive salivary eHSP70 is secreted from the ductal cells.…”