Chapter 13 Identification of antigenic phenotypes characterising lung tumour cell lines in vitro

Reeve, Julie G.; Shaw, J; Twentyman, Peter R.; Bleehen, Norman M.

doi:10.1016/s0169-5002(88)80015-1

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the findings presented here, it can be concluded that the antigen expression is not associated with a certain histological subtype of SCLC. However, preliminary evidence from in vitro studies suggests that both antigens are restricted in their expression to the classic type of SCLC cell lines (Reeve et al, 1988;Carney & Stahel, unpublished results). In addition to SCLC, a large number of other pulmonary and extrapulmonary tumour cell lines and a limited number of pulmonary and extrapulmonary tumour tissues have been previously examined for expression of CL-5 and CL-5A antigens (Stahel et al, 1986;Waibel et al, 1988).…”

Section: Discussionmentioning

confidence: 98%

Expression of the small cell carcinoma antigens of cluster-5 and cluster-5A in primary lung tumours

Maier

Schmidt²,

Waibel³

et al. 1989

Br J Cancer

View full text Add to dashboard Cite

Summary The expression of the small cell carcinoma (SCLC) antigens cluster-5 (antibody LAM8) and cluster-5A (antibody SWA20) was examined on a panel of routinely processed biopsy or surgical specimens of 290 lung tumours by immunoperoxidase staining. Antigen expression was largely restricted to SCLC. Of over 150 tissue samples evaluated, moderate or strong antigen expression was found in 49% (cluster-5) and 45% (cluster-5A). Concordance in expression of the two antigens was seen in 71% of SCLC samples, with 35% expressing both antigens strongly, 8% moderately and 28% being negative for both antigens. Antigen expression was independent of the morphological subtype of SCLC. (Stahel et al., 1985), antigens shared with white blood cells (Bunn et al., 1985;Cailland et al., 1984), neuroendocrine antigens (De Leij et al., 1985;Takahashi et al., 1986;Watanabe et al., 1987) and tumour-associated antigens (Waibel et al., 1987;Waibel et al., 1988). In the past, it has been very difficult to evaluate individual antibodies based on the original reports, since different laboratories varied widely on the material and methods used for the description of antibody and antigens. Since the First International Workshop for Small Cell Lung Cancer Antigens (Souhami et al., 1987), where antibodies have been analysed comparatively and five clusters of membrane antigens have been identified, the evaluation and reporting of individual antibodies has been greatly facilitated.We have previously reported on the characteristics of two antibodies, the IgM antibody LAM8 and the IgG2a antibody SWA20, which recognise closely related, but not identical membrane sialoglycoproteins heterogeneously expressed in SCLC (Stahel et al., 1986;Waibel et al., 1987Waibel et al., , 1988. By immunoperoxidase technique both antibodies stained equally well frozen and formalin fixed tissues of SCLC, while little or no reactivity was found with non-small cell carcinomas of lung, extrapulmonary carcinomas, and normal tissues, including bronchial epithelium and epithelium of the gastrointestinal tract, liver, kidney, pancreas, lymphoid organs, bone marrow, peripheral nerve and brain. Because of the virtual absence of expression in normal tissues, the antigens were called tumour-associated antigens of SCLC. In the First International Workshop on Small Cell Lung Cancer Antigens, the antibody LAM8 was designated to SCLC cluster-5 (CL-5) and the antibody SWA20 to SCLC cluster-5 associated (CL-5A) (Beverly et al., 1988).The aim of this study was to determine whether these two antibodies. could be used in formalin-fixed, paraffinembedded biopsy and surgical tissues obtained in a routine setting, and whether their restrictive reactivity with SCLC Correspondence: R.A. Stahel. Received 19 October 1988, and in revised form, 15 December 1988. could be confirmed when larger numbers of tissues of all types of primary lung tumours were examined. Materials and methods Tumour tissuesTumour tissues were obtained from files of the Institute of Pathology, Ruhr-University of Bochum,...

show abstract

Section: Discussionmentioning

confidence: 98%

Expression of the small cell carcinoma antigens of cluster-5 and cluster-5A in primary lung tumours

Maier

Schmidt²,

Waibel³

et al. 1989

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Several MAbs to SC-2 determinants (including AUAI) were used in indirect immunofluorescence assays by Reeve and coworkers to examine the relative antigen density of classic and variant SCLC cell lines (Reeve et al, 1988). Two of the three morphologic variant SCLC lines examined were negative for SC-2 MAb reactivity, while all eight classic SCLC lines demonstrated a positive reaction.…”

Section: Discussionmentioning

confidence: 99%

Modulation of neuroendocrine surface antigens in oncogene-activated small cell lung cancer lines

Doyle

Mabry²,

Stahel³

et al. 1991

Lung Cancer

View full text Add to dashboard Cite

Chapter 13 Identification of antigenic phenotypes characterising lung tumour cell lines in vitro

Cited by 2 publications

References 5 publications

Expression of the small cell carcinoma antigens of cluster-5 and cluster-5A in primary lung tumours

Expression of the small cell carcinoma antigens of cluster-5 and cluster-5A in primary lung tumours

Modulation of neuroendocrine surface antigens in oncogene-activated small cell lung cancer lines

Contact Info

Product

Resources

About