Chapter 15: Central effects of tricyclic compounds on the endocrine system - an in vitro study

Julesz, J.; Gálfi, Márta; Molnár, Joséph; Vecsernyés, Miklós

doi:10.1016/s0079-6123(08)62322-5

Cited by 12 publications

(2 citation statements)

References 8 publications

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“…Anterior pituitary tissue culture. Monolayer pituitary cell cultures of Wistar rats weighing 180-230 g were prepared (19). Ten pituitaries were used in every tissue culture experiment.…”

Section: Methodsmentioning

confidence: 99%

Vasopressin pressor receptor-mediated activation of HPA axis by acute ethanol stress in rats

László¹,

Varga²,

Pávó

et al. 2001

American Journal of Physiology-Regulatory, Integrative and Comp

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The plasma arginine vasopressin (AVP), ACTH, and corticosterone levels and the hypothalamic corticotropin-releasing hormone (CRH) content were measured after oral administration of 1 ml of 75% ethanol to rats, a model known to induce acute gastric erosions and stress. Elevated plasma AVP, ACTH, and corticosterone levels were detected 1 h after ethanol administration. Treatment with the vasopressin pressor (V(1)) receptor antagonist [d(CH(2))(5)Tyr(Me)-AVP] before ethanol administration significantly reduced the ACTH and corticosterone level increases. A higher hypothalamic CRH content was measured at 30 or 60 min after ethanol administration. V(1) receptor antagonist injection, 5 min before ethanol administration, inhibited the rise in hypothalamic CRH content. The protein synthesis blocker cycloheximide prevented the hypothalamic CRH content elevation after stress. The AVP-, CRH-, and AVP + CRH-induced in vitro ACTH release in normal anterior pituitary tissue cultures was also prevented by pretreatment with the V(1) receptor antagonist. The results support the hypothesis that stress-induced AVP may not only act directly on the ACTH producing anterior pituitary cells but also indirectly at the hypothalamic level via the synthesis and release of CRH.

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“…Anterior pituitary tissue culture. Monolayer pituitary cell cultures of Wistar rats weighing 180-230 g were prepared (19). Ten pituitaries were used in every tissue culture experiment.…”

Section: Methodsmentioning

confidence: 99%

Vasopressin pressor receptor-mediated activation of HPA axis by acute ethanol stress in rats

László¹,

Varga²,

Pávó

et al. 2001

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Details of the cell culture procedure were described earlier (Laszlo et al 1989;Huszti et al 1990;Julesz et al 1992;Janaky et al 1998). Under sterile conditions, the pituitary of male Wistar rats weighing 180-230 g was removed under pentobarbital anesthesia (4.5 mg/kg Nembutal, Abbott, USA), immediately after decapitation.…”

Section: Cell Culture Techniquementioning

confidence: 99%

Inhibitory Effect of Galanin on Adrenaline- and Noradrenaline-Induced Increased Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

et al. 2010

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The effects of the interactions between the 29 amino acid-containing peptide galanin and adrenaline or noradrenaline on the secretion of oxytocin were studied in 13- to 14-day cultures of isolated rat neurohypophyseal tissue. The alpha-receptor antagonist corynanthine blocked the adrenaline-induced increase of oxytocin secretion. When the beta-receptor antagonist propranolol was added before the noradrenaline treatment, the antagonist prevented the noradrenaline-induced enhancement of oxytocin release. Following the addition of galanin, the extent of oxytocin secretion into the supernatant medium decreased. Adrenaline and noradrenaline treatments increased the oxytocin level. Preincubation with galanin reduced the adrenaline- and noradrenaline-induced oxytocin level elevations. The blocking effect of galanin was prevented by previous treatment with the galanin receptor antagonist galantid (M15). When adrenaline or noradrenaline treatment was applied before galanin addition, the oxytocin secretion remained enhanced. The present results indicate that the changes in oxytocin secretion induced by the adrenergic system can be directly influenced by the galaninergic system. The interactions between the adrenergic and galaninergic systems from the aspect of oxytocin secretion can occur at the level of the posterior pituitary, independently of the hypothalamus.

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Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

et al. 2016

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The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13-14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of vasopressin and oxytocin production were detected in the cell culture media following ghrelin administration, depending on the ghrelin doses. The oxytocin level proved to be more elevated than that of vasopressin. The increase of vasopressin and oxytocin secretion could be totally blocked by previous administration of the ghrelin receptor antagonist ([D-Lys]-growth hormone-releasing peptide-6). Application of the ghrelin receptor antagonist after ghrelin administration proved ineffective. The results indicate that vasopressin and oxytocin release is influenced directly by the ghrelin system, and the effects of ghrelin on vasopressin and oxytocin secretion from the neurohypophyseal tissue in rats can occur at the level of the posterior pituitary. Our observations lend support to the view that neurohypophysis contains ghrelin receptors.

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Chapter 15: Central effects of tricyclic compounds on the endocrine system - an in vitro study

Cited by 12 publications

References 8 publications

Vasopressin pressor receptor-mediated activation of HPA axis by acute ethanol stress in rats

Vasopressin pressor receptor-mediated activation of HPA axis by acute ethanol stress in rats

Inhibitory Effect of Galanin on Adrenaline- and Noradrenaline-Induced Increased Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures

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