As in bacteriology, the goals of a good mycology laboratory are to accurately isolate and identify fungi suspected of causing infection. It is the laboratory's responsibility to ensure that specimens are appropriate for the requested test and to provide submitters with the guidelines for proper specimen selection, collection, and transport to the laboratory. This chapter provides general information related to fungal culture from various specimens and is based on historical practice that has been successful. Pretreatment of several specimen types is necessary to maximize the recovery of fungi. Abscess specimens should be collected from the active peripheral edge of open abscesses or aspirated from closed abscesses by use of a syringe. There are presently a wide variety of manual, biphasic, automated, and continuously monitoring systems for blood cultures, but no single commercial system or culture medium can detect all potential blood pathogens.