Chapter 2 Methods of Culture for Plasmodial Myxomycetes

Daniel, John W.; Baldwin, Helen H.

doi:10.1016/s0091-679x(08)62084-1

Cited by 287 publications

(169 citation statements)

References 51 publications

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“…Strain TU291 was a gift from Dr. Philipe Albert, Cytobiology Laboratory of Reims University, France, and the culture method was referred to Daniel and Baldwin [21].…”

Section: Strain Resource and Culture Methodsmentioning

confidence: 99%

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

et al. 1999

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The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrix and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrix and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in them. Immunodotting results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic observations further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.

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“…Strain TU291 was a gift from Dr. Philipe Albert, Cytobiology Laboratory of Reims University, France, and the culture method was referred to Daniel and Baldwin [21].…”

Section: Strain Resource and Culture Methodsmentioning

confidence: 99%

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

et al. 1999

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“…Cultures (microplasmodia) were grown in submerged culture on the semi-defined medium as previously described [1]. Microplasmodia are prepared for measurement by low speed centrifugation followed by washing three times with 10-fold the pellet volumes of 0.15 M sucrose-10 mM KCI each time with final resuspension in the same medium, 50 ml per ml packed pellet.…”

Section: Methodsmentioning

confidence: 99%

“…All pH experiments were performed in dim room light. Protein was measured by a modified Lowry method [5] and the yellow pigments as previously described [ 1 ]. Double glass-distilled water was used for all procedures except for growing the cultures.…”

Section: Methodsmentioning

confidence: 99%

“…Light also strongly and rapidly inhibits submerged culture growth of this organism [1,2] suggesting the possibility that the primary light-dependent reactions assumed to initiate the sequence of differentiation reactions is present in at least these two stages of the life cycle. Previously, long term metabolic changes, for example, transient increases in plasmodial ATP and in a glycogen-like polysaccharide as well as a sustained and large increase in the pH of the culture medium were observed [3].…”

Section: Introductionmentioning

confidence: 99%

“…Light is required to induce the gametic sporulation of suitably starved plasmodia of the coenocyte Physarum polycephalum [ 1]. Light also strongly and rapidly inhibits submerged culture growth of this organism [1,2] suggesting the possibility that the primary light-dependent reactions assumed to initiate the sequence of differentiation reactions is present in at least these two stages of the life cycle.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of light on the permeability of a myxomycete

Daniel

Eustace

1972

FEBS Letters

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Effects of medium composition on the growth and lipid production of microplasmodia of Physarum polycephalum

Truong

Stephenson

Phung

et al. 2019

Biotechnology Progress

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Physarum polycephalum is a plasmodial slime mold. One of the trophic stages in the life cycle of this organism is a plasmodium. In submerged culture, plasmodia are fragmented into microplasmodia. The latter both lack cell walls and are capable of rapid growth. There has been limited information on the effects of medium composition on the growth and lipid accumulation of microplasmodia. In this study, optimization of medium components by response surface methodology showed that tryptone and yeast extract concentrations had the most significant effects on lipid and biomass production; significant synergistic interactions between glucose and tryptone concentration on these responses were also recorded. The optimal medium was composed of 20 g/L of glucose, 6.59 g/L of tryptone, and 3.0 g/L of yeast extract. This medium yielded 13.86 g/L of dry biomass and 1.97 g/L of lipids. These amounts are threefold higher than those of the American Type Culture Collection (ATCC) medium. In addition, biomass and lipid production reached maximal values between only 4 and 5 days. Fatty acid compositions analysis by gas chromatography‐mass spectrometer (GC–MS) revealed that P. polycephalum lipids consisted mainly of oleic acid (40.5%), linoleic acid (10%), and octadecynoic (15.8%). This is the first report on the fatty acid composition of P. polycephalum microplasmodia. These results suggest that the biomass of microplasmodia could be used as a source of material for direct conversion into biodiesel because of the absence of cell walls or it could also be used as a supplemental source of beneficial fatty acids for humans, albeit with some further evaluation needed.

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Chapter 2 Methods of Culture for Plasmodial Myxomycetes

Cited by 287 publications

References 51 publications

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

The effect of light on the permeability of a myxomycete

Effects of medium composition on the growth and lipid production of microplasmodia of Physarum polycephalum

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