Chapter 4 A web-accessible digital atlas of the distribution of nitric oxide synthase in the mouse brain

Cork, R. John; Perrone, Marly; Bridges, Daniel; Wandell, J.; Scheiner, C.A.; Mize, R. Ranney

doi:10.1016/s0079-6123(08)63199-4

Cited by 40 publications

(29 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The NO-producing system in the brain of B6EiC3HF1 mice, and the other two strains, is well developed, widely diffused, and distinct from other neurochemically identified cell populations. Our results are in general in harmony with those of previous studies, performed both with immunohistochemistry or histochemistry, in rats (Vincent and Kimura, 1992;Yamada et al, 1996) and C57BL/6J mice (Cork et al, 1998).…”

Section: Pons Medulla Oblongata and Cerebellumsupporting

confidence: 92%

“…In the more caudal portion of the cortex the cells were of larger size and were located mainly in the deeper layers. The presence of NO-producing elements in the cerebral cortex was earlier suggested after ND studies, when intensely labeled neurons were found throughout the cerebral cortex of rat and mouse (Cork et al, 1998;Vincent and Kimura, 1992). In these two species, a clear colocalization of nNOS and ND was observed only in a subset of interneurons (Bredt et al, 1991;Kharazia et al, 1994;Oermann et al, 1999).…”

Section: Cortexmentioning

confidence: 60%

“…More recently, a digital atlas of the distribution of NOS activity in the C57/BL6J mouse brain, using ND histochemistry, was published (Cork et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distribution of nitric oxide synthase immunoreactivity in the mouse brain

Gotti

Sica

Panzica

2005

Microsc. Res. Tech.

View full text Add to dashboard Cite

Nitric oxide (NO) is a gaseous intercellular messenger with a wide range of neural functions. NO is synthesized by activation of different isoforms of nitric oxide synthases (NOS). At present NOS immunoreactivity has been described in mouse brain in restricted and definite areas and no detailed mapping studies have yet been reported for NOS immunoreactivity. We have studied the distribution of neuronal NOS-containing neurons in the brain of three months male mice, using a specific commercial polyclonal antibody against the neuronal isoform of nitric oxide synthase (nNOS). Neuronal cell bodies exhibiting nNOS immunoreactivity were found in several distinct nuclei throughout the brain. The neurons that were positively stained exhibited different intensities of reaction. In some brain areas (i.e., cortex, striatum, tegmental nuclei) neurons were intensely stained in a Golgi-like fashion. In other regions, immunoreactive cells are moderately stained (i.e., magnocellular nucleus of the posterior commissure, amygdaloid nucleus, interpeduncular nucleus, lateral periaqueductal gray) or weakly stained (i.e., vascular organ of the lamina terminalis, hippocampus, inferior colliculus, reticular nucleus). In the mouse, the NO-producing system appears well developed and widely diffused. In particular, nNOS immunoreactive neurons seem chiefly present in several sensory pathways like all the nuclei of the olfactory system, as well as in many regions of the lymbic system. These data suggest a widespread role for the NO system in the mouse nervous system.

show abstract

Section: Pons Medulla Oblongata and Cerebellumsupporting

confidence: 92%

Section: Cortexmentioning

confidence: 60%

See 1 more Smart Citation

Distribution of nitric oxide synthase immunoreactivity in the mouse brain

Gotti

Sica

Panzica

2005

Microsc. Res. Tech.

View full text Add to dashboard Cite

show abstract

“…Confirming the regional relationship between levels of sGC and NOS previously reported (Ibarra et al, 2001), we found especially high levels of sGC in the neostriatum, olfactory tubercle, and supraoptic nucleus, three brain structures that also express high level of NOS (Vincent and Kimura, 1992;Cork et al, 1998); conversely, regions low in sGC generally lacked significant levels of NOS. Fig.…”

Section: Relationship Between Sgc and Nos-isupporting

confidence: 89%

Distribution of soluble guanylyl cyclase in the rat brain

Ding

Burette

Nedvetsky³

et al. 2004

J of Comparative Neurology

View full text Add to dashboard Cite

The diffusible messenger nitric oxide (NO) acts in the brain largely through activation of soluble guanylyl cyclase (sGC), a heterodimer comprising alpha and beta subunits. We used immunohistochemistry to study the distribution of both sGC subunits in the brain of adult rats. alpha and beta subunits gave similar widespread staining throughout the CNS, which was strongest in neostriatum, olfactory tubercle, and supraoptic nucleus. Double-labeling experiments showed striking cellular colocalization in most brain regions, suggesting that the two subunits may be organized into enzymatically active alpha/beta heteromers. Mismatches were observed in cerebellar cortex: Purkinje cells and Bergmann glia were positive for both subunits, whereas granule cells and interneurons in the molecular layer were strongly immunopositive for beta but only weakly stained for the alpha subunit. By using multiple labeling, we compared the localization of sGC with neuronal nitric oxide synthase (NOS-I, the NO-producing enzyme in neurons). In forebrain, the distribution of sGC and NOS-I was complementary, with only occasional colocalization. In contrast, cellular colocalization was common in midbrain and cerebellum. These data support a widespread role for the NO/sGC/cGMP pathway in the CNS and suggest that, in addition to its role as paracrine messenger, NO may also be an intracellular autocrine agent.

show abstract

“…In some parts of the mouse brain, the distributions of NADPH-d-positive cell bodies and fibers were reported and shown in photomicrographs; in the olfactory bulb [31,67], cortex [43,44], and cerebellar cortex [19]. The distribution of the NADPH-d-positive neuronal cell bodies in the mouse brain is reported in a web-accessible digital atlas [5], but the atlas does not have detailed mapping of the NADPH-d-positive fibers. To date, there has been no histochemical study of the distribution of neuronal NADPHd-positive cell bodies and fibers throughout the mouse brain including a schematic map.…”

Section: Introductionmentioning

confidence: 99%