Chapter 6 Emerging Techniques: Enzyme-Linked Immunosorbent Assay (ELISA) as Alternatives to Chromatographic Methods

Ward, C. M.; Wilkinson, A. W.; Morgan, Michael R. A.

doi:10.1016/s0301-4770(08)60566-7

Cited by 1 publication

(2 citation statements)

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“…Immunochemical methods have been developed for the determination of several mycotoxins including the aflatoxins, ochratoxin A, cyclopiazonic acid, rubratoxin, zearalanone, sterigmatocystin, and various trichothecenes (Chu, 1986;Ward et al, 1993 (PAb) against the fumonisins from the splenic lyphocytes of mice immunized with a FB 1 -cholera toxin conjugate. A MAb-based competitive direct enzymelinked immunosorbent assay (CD-ELISA) was developed, whereby FB 1 -horseradish peroxidase conjugate and free FB 1 competed for antibody binding sites immobilized on microtiter plates (Ascona-Olivera et al, 1992b).…”

Section: Introductionmentioning

confidence: 99%

“…Immunochemical techniques for the determination of mycotoxins are generally rapid, matrix independent, and easy to apply in both the laboratory and field environments, exhibiting comparable sensitivity and higher selectivity characteristics than corresponding chromatographic methods (Chu, 1986;Ward et al, 1993). Immunochemical methods have been developed for the determination of several mycotoxins including the aflatoxins, ochratoxin A, cyclopiazonic acid, rubratoxin, zearalanone, sterigmatocystin, and various trichothecenes (Chu, 1986;Ward et al, 1993 (PAb) against the fumonisins from the splenic lyphocytes of mice immunized with a FB 1 -cholera toxin conjugate. A MAb-based competitive direct enzymelinked immunosorbent assay (CD-ELISA) was developed, whereby FB 1 -horseradish peroxidase conjugate and free FB 1 competed for antibody binding sites immobilized on microtiter plates (Ascona-Olivera et al, 1992b).…”

Section: Introductionmentioning

confidence: 99%

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Determination of Fumonisins in Corn: Evaluation of Competitive Immunoassay and HPLC Techniques

Sydenham¹,

Shephard²,

Thiel³

et al. 1996

J. Agric. Food Chem.

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The fumonisins, mycotoxins produced by Fusarium moniliforme, are known to occur as natural contaminants of corn worldwide and to be associated with several animal disease syndromes. Highperformance liquid chromatography (HPLC) and monoclonal antibody-based (MAb) competitive direct enzyme-linked immunosorbent assay (CD-ELISA) methods, developed for the determination of fumonisins in corn, were compared. CD-ELISA results for naturally contaminated corn were consistently higher than corresponding HPLC results. Quantitative differences were reduced by decreasing the organic phase composition of the extract and by introducing a hexane partitioning step. The results were indicative of a possible lipid-based matrix effect, but when applied to fumonisin-free corn spiked with fumonisin levels ranging from 0.8 to 12.8 µg/g, analyses by both techniques were well correlated (r ) 0.996). It is concluded that structurally related fumonisinlike compounds, present in naturally contaminated corn, may contribute to the differences recorded between the two methods, although the MAb-based CD-ELISA may still be used as an initial semiquantitative screening technique.

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Section: Introductionmentioning

confidence: 99%