Chapter 7 Roles of milk-borne components in the development of pancreatic enzymes during the suckling period

Kinouchi, Toshi; Yajima, Takaji

doi:10.1016/s1877-1823(09)70123-7

Cited by 1 publication

(1 citation statement)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An explanation to the inconsistency in the effect of PHA on the two pancreatic enzymes could be the involvement of several different hormones in the regulation of different digestive enzymes. It has been shown that insulin is a major stimulator of the pancreatic amylase gene expression, and the plasma insulin level changes in parallel with the amylase activity in the pancreas during the third postnatal week in the rat [22,23] . However, previous results have failed to demonstrate that the plasma-insulin levels are affected by enteral PHA treatment on acute exposure in suckling rats [20] .…”

Section: Pha Effect On the Pancreasmentioning

confidence: 99%

Enterally but Not Parenterally Administered <i>Phaseolus vulgaris </i>Lectin Induces Growth and Precocious Maturation of the Gut in Suckling Rats

Linderoth¹,

Prykhodko²,

Pierzynowski³

et al. 2006

Neonatology

View full text Add to dashboard Cite

Background: The lectin, phytohemagglutinin (PHA) has been shown to induce growth and functional maturation of the gastrointestinal (GI) tract in suckling rats. Objectives: To investigate the effect of the administration route, and whether enteral exposure to PHA was necessary to induce functional maturation. Methods: Fourteen-day-old rats were daily administered PHA via orogastric feeding (0.05 mg PHA/g BW) or via subcutaneous injection (0.05 or 0.005 mg PHA/g BW) for 3 days, while the controls received saline orogastrically. At 17 days of age, organ weight, intestinal and pancreatic function, and plasma corticosterone levels were analyzed. Moreover, 14-days old pups receiving a single dose of PHA, enterally or parenterally, were sacrificed after 12 h and examined for organ PHA binding using immunohistochemistry. Results: Enteral PHA exposure resulted in PHA binding in the epithelial lining of the small intestine, increased gastrointestinal growth, reduced intestinal macromolecular absorption, altered the disaccharidase expression towards an adult-like pattern, and increased the pancreatic protein and trypsin contents. In contrast, parenteral PHA exposure (high dose) resulted in PHA-binding in extra-intestinal organs, increased liver and spleen weight, and decreased thymus weight. Moreover, the intestinal maltase activity increased moderately, and the transfer of BSA to blood plasma was partially reduced. Both PHA treatments led to elevated plasma corticosterone levels. Conclusion: These results demonstrated that enteral exposure to PHA was necessary to induce the precocious maturation of the GI tract and the pancreas, while parenteral administration affects the extra-intestinal organs. Furthermore, the enteral effects were probably not mediated via a corticosteroid dependent pathway.

show abstract

Section: Pha Effect On the Pancreasmentioning

confidence: 99%