Chapter 8 Biocatalytic applications of autotrophic organisms

Grimm, Hanna C.; Erdem, Elif; Kourist, Robert

doi:10.1515/9783110550603-008

Cited by 4 publications

(3 citation statements)

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“…Particularly, their capacity to utilise light for the biotransformation of specific substrates into the desired products is a unique advantage compared to heterotrophic bacteria. Some studies also found the reductive ability of in vivo biotransformations using terpenes, hydrocortisone, chalcones, and oxophosphonates [ 198 , 199 ]. Studies of photobiocatalysis were also performed using microalgae like C. reinhardtii [ 200 ].…”

Section: Photolysis Photocatalytic and Photobiocatalytic Transformati...mentioning

confidence: 99%

Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review

Barone,

Rodríguez-Seijo,

Parati

et al. 2024

Environmental Science and Ecotechnology

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Section: Photolysis Photocatalytic and Photobiocatalytic Transformati...mentioning

confidence: 99%

Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review

Barone,

Rodríguez-Seijo,

Parati

et al. 2024

Environmental Science and Ecotechnology

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“…Oxyfunctionalization reactions belong to the most important transformations in organic chemistry. Oxyfunctionalization involves integration of at least one oxygen atom into the product [18]. Conventionally, reactions involving oxygen require high reaction temperatures and/or pressures.…”

Section: Oxyfunctionalizationmentioning

confidence: 99%

“…Due to these constraints, photobiotransformations are usually employed at small scale (V 10 mL) and at low cell densities (< 10 g CDW L -1 ). The latter requirement is due to ''self-shading'' where cells obscure each other resulting in non-homogeneous light distribution [18]. Other important factors to consider are photoinhibition, CO 2 dispersion as well as O 2 degassing [84].…”

Section: Internally Illuminated Photobioreactorsmentioning

confidence: 99%

Recent Advances in Cyanobacterial Biotransformations

Malihan‐Yap

Grimm

Kourist

2022

Chemie Ingenieur Technik

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Light-driven biotransformations in recombinant cyanobacteria have been explored in recent years for the production of chiral compounds and platform chemicals. In particular, cyanobacteria harboring oxidoreductases proved to be sustainable hosts providing and recycling reducing equivalents and improving the atom economy of the reactions. However, the provision of light in photobioreactors is considered to be the major bottleneck for up-scaling. In this review, genetic tools for generating recombinant cyanobacterial strains and up to date cyanobacterial biotransformations including redesigning of the photosynthetic electron transport chain to increase specific activities are presented. Finally, several photobioreactor geometries to circumvent the light limitation are discussed and some future perspectives are presented.

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Sucrose as an electron source for cofactor regeneration in recombinant Escherichia coli expressing invertase and a Baeyer Villiger monooxygenase

Sovic,

Malihan-Yap,

Tóth

et al. 2024

Microb Cell Fact

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Background The large-scale biocatalytic application of oxidoreductases requires systems for a cost-effective and efficient regeneration of redox cofactors. These represent the major bottleneck for industrial bioproduction and an important cost factor. In this work, co-expression of the genes of invertase and a Baeyer–Villiger monooxygenase from Burkholderia xenovorans to E. coli W ΔcscR and E. coli BL21 (DE3) enabled efficient biotransformation of cyclohexanone to the polymer precursor, ε-caprolactone using sucrose as electron source for regeneration of redox cofactors, at rates comparable to glucose. E. coli W ΔcscR has a native csc regulon enabling sucrose utilization and is deregulated via deletion of the repressor gene (cscR), thus enabling sucrose uptake even at concentrations below 6 mM (2 g L−1). On the other hand, E. coli BL21 (DE3), which is widely used as an expression host does not contain a csc regulon. Results Herein, we show a proof of concept where the co-expression of invertase for both E. coli hosts was sufficient for efficient sucrose utilization to sustain cofactor regeneration in the Baeyer–Villiger oxidation of cyclohexanone. Using E. coli W ΔcscR, a specific activity of 37 U gDCW−1 was obtained, demonstrating the suitability of the strain for recombinant gene co-expression and subsequent whole-cell biotransformation. In addition, the same co-expression cassette was transferred and investigated with E. coli BL21 (DE3), which showed a specific activity of 17 U gDCW− 1. Finally, biotransformation using photosynthetically-derived sucrose from Synechocystis S02 with E. coli W ΔcscR expressing BVMO showed complete conversion of cyclohexanone after 3 h, especially with the strain expressing the invertase gene in the periplasm. Conclusions Results show that sucrose can be an alternative electron source to drive whole-cell biotransformations in recombinant E. coli strains opening novel strategies for sustainable chemical production.

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Chapter 8 Biocatalytic applications of autotrophic organisms

Cited by 4 publications

References 0 publications

Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review

Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review

Recent Advances in Cyanobacterial Biotransformations

Sucrose as an electron source for cofactor regeneration in recombinant Escherichia coli expressing invertase and a Baeyer Villiger monooxygenase

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