Chapter 8: Chemical ecology of sand fly plant-feeding behaviour

Torto, Baldwyn; Hassaballa, Iman B.; Tchouassi, David P.

doi:10.3920/978-90-8686-932-9_8

Cited by 4 publications

(3 citation statements)

References 78 publications

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“…As such, it is essential to embrace alternative ways to control vector populations. For example, semiochemical-baited traps, especially those that mediate oviposition represents promising complementary tools for mosquito control (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

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Bacteria associated with Parthenium hysterophorus root exudate influence olfactory oviposition responses of Anopheles gambiae

Milugo,

Torto,

Tchouassi

2024

Front. Trop. Dis

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IntroductionPreviously, we documented that the malaria vector Anopheles gambiae responds to volatile emissions from the root exudate water of the invasive plant, Parthenium hysterophorus. However, the origin of the volatiles remains to be investigated. Here, we isolated bacteria from the root exudate water of the plant, test the influence of their volatiles in gravid An. gambiae oviposition, and examined relationships between volatile profiles and oviposition.MethodsBacteria from root exudate water of P. hysterophorus were isolated using culture on Luria Bertani medium and identified by sequencing the 16S rRNA gene. Cultures of individual isolates were evaluated for egg laying response by gravid An. gambiae and number of eggs laid compared using generalized linear models relative to those in crude bacteria-mixture. Headspace volatile emissions of the bacterial isolates were analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and relationships between volatile organic compound (VOC) profiles and gravid mosquito oviposition examined using Random Forest Analysis. Proximate analysis was performed to assess the difference in volatile chemistry among the different isolates.ResultsThree isolates were identified as Gram-negative bacteria belonging to two families: Enterobacteriaceae (Enterobacter sp. and Enterobacter mori) and Alcaligenaceae (Alcaligens aquatilis). An. gambiae laid 3-fold more eggs in cultures of A. aquatilis than in those of Enterobacter sp. In turn, approx. 4-fold more eggs were laid in cultures of E. mori than A. aquatilis. Overall, 16 VOCs were identified in the headspace of the isolates belonging to the chemical classes benzenoids, pyrazines, aldehydes, terpenes, alcohols, alkanes, and indoles. Random Forest Analysis identified 10 compounds contributing the most to the attraction of odors of the bacteria isolates to oviposition. Specifically, dodecane and indole were emitted in higher amounts in odors of Enterobacter sp than the other two species. Proximate analysis revealed differential attraction of the isolates on the gravid mosquito to be associated with their volatile profiles.ConclusionOur results provide first report of E. mori or A. aquatilis mediating attractive oviposition responses in An. gambiae in support of the important role microbes play in insect oviposition. The potential use of the microbes and associated volatiles in malaria vector management needs further investigation.

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Section: Introductionmentioning

confidence: 99%

“…To ensure reproductive success, gravid mosquitoes are known to identify and select oviposition sites that can support the growth and survival of their offspring (9,10). Detection of these preferred oviposition environments by gravid mosquitoes is achieved by integrating a range of cues including olfactory, tactile and visual cues (9).…”

Section: Introductionmentioning

confidence: 99%

Bacteria associated with Parthenium hysterophorus root exudate influence olfactory oviposition responses of Anopheles gambiae

Milugo,

Torto,

Tchouassi

2024

Front. Trop. Dis

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“…Phlebotomine sand flies are vectors of Leishmania parasites and cause leishmaniasis that afflict humans besides other bacterial and viral pathogens (22,23). To date, only a handful of studies have assessed the behavioral response of 1-octen-3-ol (racemic mixture) on sandflies.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-catalyzed kinetic resolution of racemic 1-octen-3-ol and field evaluation of its enantiomeric isomers as attractants of sandflies

Tchouassi,

Jacob,

Cheseto

et al. 2024

Front. Trop. Dis

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Phlebotomine sand flies are medically important as vectors of the protozoan parasites that cause leishmaniasis and other bacterial and viral pathogens. Previous work demonstrated that both sexes of certain species of sandflies are attracted to 1-octen-3-ol (octenol). Since 1-octen-3-ol exists as two enantiomeric isomers ─ ((R)-(-)- (R-form) and (S)-(+)- (S-form), we tested the hypothesis that the two enantiomeric forms and racemic mixture (R/S) attracted different sand fly species. We carried out field trials in a leishmaniasis endemic foci in Baringo County, Kenya. In a randomized design, trap captures of sandflies in CDC light traps baited with the R-, S- and racemic (R/S) forms of 1-octen-3-ol in hexane varied with the form and dose of the compound. Interestingly, of the captured species, only Phlebotomus martini, the vector of the parasite causing visceral leishmaniasis, exhibited a dose-dependent response to octenol; captures of both sexes of the species being generally 1.7-fold higher with the R- than S-form. There was no significant effect of treatment on captures of Sergentomyia species (S. schwetzi, S. antennata, S, clydei). Our findings have implications for surveillance of sandfly populations as part of leishmaniasis epidemiologic investigation.

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Chapter 1: Resource location in a complex sensory landscape

Hopkins

2022

Sensory Ecology of Disease Vectors

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Chapter 8: Chemical ecology of sand fly plant-feeding behaviour

Cited by 4 publications

References 78 publications

Bacteria associated with Parthenium hysterophorus root exudate influence olfactory oviposition responses of Anopheles gambiae

Bacteria associated with Parthenium hysterophorus root exudate influence olfactory oviposition responses of Anopheles gambiae

Enzyme-catalyzed kinetic resolution of racemic 1-octen-3-ol and field evaluation of its enantiomeric isomers as attractants of sandflies

Chapter 1: Resource location in a complex sensory landscape

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