Chapter 9 Fusion of Viral Envelopes with Cellular Membranes

Ohnishi, Shun‐ichi

doi:10.1016/s0070-2161(08)60137-9

Cited by 67 publications

(46 citation statements)

References 139 publications

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“…Second, fusion of viral and plasma membranes occurs quickly (within 1-2 min after binding). 21 Third, liposomes composed of anionic lipid are able to hold 500-1000 molecules of plasmid DNA per liposome. 17 These features seem to be important for a high transduction rate in vivo.…”

Section: Discussionmentioning

confidence: 99%

Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-liposome

et al. 1998

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Most viral vectors are highly immunogenic and are of limthirds of the liver by histological staining. It was found that ited use for somatic gene therapy that requires repetitive the transfection efficiency was not affected by repetitive administrations. We have developed a highly efficient gene transfections. In support of these findings, antibody transduction procedure useful for repetitive transfections response to HVJ-liposome detected in the rat sera was using liposome containing hemagglutinating virus of Japan weak and transient. Furthermore, cytotoxic T lymphocytes (HVJ-liposome). The Escherichia coli ␤-galactosidase (␤-were not elicited against autologous rat hepatocytes that gal) gene was embodied in HVJ-liposome, and introduced were transfected in vivo using HVJ-liposome. Thus, our directly into the caudal lobe of rat liver that was transiently results demonstrate that the isolation of a target liver from isolated from a systemic circulation. A 116 kDa ␤-gal prosystemic circulation and the direct administration of foreign tein was detected in transfected rat liver tissues by Westgenes using HVJ-liposomes are useful for high gene transern blot analysis and it was expressed in more than twoduction and persistent gene expression in the liver.

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Section: Discussionmentioning

confidence: 99%

Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-liposome

et al. 1998

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“…These changes in the conformation of G protein are thought to associate with the fusion event (2,11,12), but the molecular mechanisms underlying VSV-induced cell fusion remain uncertain (11,(13)(14)(15).…”

Section: Discussionmentioning

confidence: 99%

VESICULAR STOMATITIS VIRUS-MEDIATED CELL FUSION SUBSEQUENT TO VIRUS ADSORPTION AT DIFFRENT pH VALUES

Nagata

Kurata

Ueno

et al. 1991

JJMSB

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SUMMARY:Vesicular stomatitis virus (VSV)-mediated cell fusion from without can be induced by transient exposure to low pH, subsequent to adsorption of VSV at neutral pH. To study the mechanism of VSV-induced cell fusion, we examined the effect of pH condition at virus adsorption on acid-inducible VSVmediated cell fusion. Although the binding of VSV to BHK-21 cells was most efficient under acidic condition (pH 5.7-6.3), extensive cell fusion was not observed under this condition. A temporary exposure to low pH after binding at neutral pH also decreased fusion activity. However, return to neutral pH for 2 min just after the acid binding restored the fusion activity. These results indicate the requirement of neutral pH condition for VSV-mediated cell fusion prior to the acid stimulation which induces conformational change of the virus glycoprotein into a fusogenic form.

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“…Finally, in negative stain EM, isolated soluble TBHA 2 has very similar dimensions to low-pH HAs on the viral surface (Ruigrok et al, 1986a;1988), which sticks out of the membrane in an upright manner. Recently, Wharton et al (1995) have determined the orientation of low-pH HA s on the viral membrane in relation to the TBHA 2 crystal-structure.…”

Section: Low-ph Induced Conformational Change In Hamentioning

confidence: 97%

Low-pH induced conformational changes in viral fusion proteins: implications for the fusion mechanism

Gaudin

Ruigrok

Brunner

1995

Journal of General Virology

119

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Chapter 9 Fusion of Viral Envelopes with Cellular Membranes

Cited by 67 publications

References 139 publications

Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-liposome

Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-liposome

VESICULAR STOMATITIS VIRUS-MEDIATED CELL FUSION SUBSEQUENT TO VIRUS ADSORPTION AT DIFFRENT pH VALUES

Low-pH induced conformational changes in viral fusion proteins: implications for the fusion mechanism

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