Chapter III Inoculation Techniques—Effects Due to Quality and Quantity of Inoculum

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

“…The inoculum quantity of normally used is between 3% and 10% of the medium volume (Lincoln 1960, Meyrath and Suchanek 1972, Hunt and Stieber 1986. A relatively large inoculum volume may be used to generate the maximum production in as short a time as possible, thus increasing the vessel productivity.…”

EFFECT OF CULTURE PARAMETERS ON PROTEASE AND CELLULASE PRODUCTION BY TWO BACTERIAL STRAINS, Corynebacterium alkanolyticum ATH3 AND Bacillus licheniformis CBH7 ISOLATED FROM FISH GUT

“…C alkanolyticum ATH3 (8.58±0.340 U) and CBH7 B. licheniformis (6.51±0.473 U) were found to be the highest at 3% inoculum size (Table 4). Inoculum quantity normally used is between 3% and 10% of the medium volume (Lincoln 1960;Meyrath & Suchanek 1972;Hunt & Stieber 1986). Lincoln (1960) stressed that bacterial inocula should be transferred in the logarithmic growth phase when the cells were still metabolically active.…”

Flow Microcalorimetric Studies of Yeast Growth: Fundamental Aspects

“…The commonly used overnight culture technique may require passaging a sample through the chosen medium before inoculation (Meyrath & Suchanek 1972) so that (i) the micro-organism may be adapted from its environmental physiological state to that in the medium of choice and (ii) an approximate cell density can be predicted. Figure 3 illustrates how different the p t curves are if there is no passaging prior to inoculation into the calorimetric medium.…”

“…The effects of inoculum density on microbial growth are well known (Dean & Hinshelwood 1966;Meyrath & Suchanek 1972;Pirt 1975;Pamment & Hall 1978). The known effects may limit the usefulness of the p-t curves as 'fingerprints' for identification purposes and illustrate the need for detailed investigation of the effects of inoculum density upon the derived p -t curves.…”