Chapter III Techniques for Microscopic Preparation

Cultural Growth Patterns and Incompatibility Reactions in Diaporthe and Phomopsis Populations

“…ml; formalin (37%) 10 ml; glacial acetic acid 5 ml), where they were allowed to remain until they sank (about 24 h). Methods for paraffin embedding, block-making, microtome-cutting and wax-removal were as descried by Dring (1971). Sections 5-8 ^m thick were stained (10-15 s) with Light Green SF Yellowish (0.5% sol in clove oil).…”

The influence of sampling duration on recovery of culturable fungi using the Andersen N6 and RCS bioaerosol samplers

“…Microscopic preparations were made in distilled water, clear lactic acid, or lactofuchsin (Carmichael, 1955) depending on Sampling duration and recovery of culturable fungi the organism examined. Fungi with delicate reproductive structures were examined by preparing microscopic mounts on clear adhesive tape placed in mounting fluid and covered with a cover slip (Dring, 1971). Several standard reference works were used during identification procedures, including: Arx (1970), Barnett and Hunter (1986), Barron (1968), de Hoog et al (2000, Domsch et al (1980), Ellis (1971Ellis ( , 1976, Hanlin (1990), Malloch (1981), Onions et al (1981), and Samson et al (1996).…”

Chlorhexidine digluconate induces mitotic recombination in diploid cells of Aspergillus nidulans

“…Chlorhexidine digluconate was added to MM plates to achieve final concentrations of 1, 5 and 10 μ M. Conidia of the diploid strain were inoculated and cultivated over dialysis membranes supported by MM + CLX. Plates were incubated at 37°C and samples were collected, stained with lactophenol cotton blue (Dring, 1971), and examined under a light microscope after 24 and 48 h of exposure.…”