Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Abstract

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Regulation of Flagellar Biogenesis by a Calcium Dependent Protein Kinase in Chlamydomonas reinhardtii

“…reinhardtii , the cloned CDPK in C . moewusii [29] was used as query to search Chlamydomonas genome. 14 CDPKs were identified that had unique CDPK features.…”

“…Flagellar or ciliary localization of CDPKs has been reported in Paramecium [27], [28], and green algae C . eugametos [29] and C. reinhardtii [30] while their physiological functions remain unknown.…”

Two Calcium-Dependent Protein Kinases fromChlamydomonas reinhardtiiare transcriptionally regulated by nutrient starvation

“…19 Another 65 kDa CDPK has been shown to be localized in the C. moewusii flagella. 41 In conclusion, the 2 CDPKs from C. reinhardtii cells are regulated by nutrient starvation (P and N); however, it was only CDPK3 that was upregulated upon acetate starvation. This might suggest a plausible Ca 2+ -dependent pathway in response to nutrient stress.…”

“…As of now, these algal CDPKs have been characterized as Ca 2+ -binding and Ca 2+ -dependent Protein Kinases. 40,41 Expression analysis of CDPK1 and CDPK3 transcripts under nutrient starvation…”

CLONING OF A cDNA ENCODING A 66‐kDa Ca2+‐DEPENDENT PROTEIN KINASE (CDPK) FROM DUNALIELLA TERTIOLECTA (CHLOROPHYTA)

“…Hence, DtCPK1 does not belong to any of the seven subfamilies as defined by Satterlee and Sussman (1998). Therefore, D. tertiolecta DtCPK1, as well as the similar CCK1, a CDPK from C. eugamentos (Siderius et al 1997), may form the eighth subfamily of CDPKs. The representative domain structure of the new subfamily of CDPK is schematically shown in Fig.…”