Characterisation and differentiation of oligotrophic waters by culturable particle-attached and free-living bacterial communities

Blažina, Maria; Najdek, Mirjana; Fuks, Dragica; Ruso, Ana; Štifanić, Mauro; Pavlinić, Dinko

doi:10.1016/j.ecolind.2009.04.005

Cited by 7 publications

(6 citation statements)

References 18 publications

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“…The free-living community could profit from the activity of the attached bacteria releasing dissolved organic matter into the water via exoenzymatic activities, providing needed substrate for the free-living microbial community (73). In addition to POM, temperature also appeared to influence the free-living fraction of the HAM.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, there are reports of Synechococcus attached to particles (72), and Malfatti and Azam (68) observed Synechococcus cells attached to one or more heterotrophic bacterial cells using atomic force microscopy (AFM), suggesting that at least some Synechococcus cells are legitimate members of an attached community. Attached Synechococcus in the HAM lagoon could benefit from the dissolved organic carbon released by their bacterial partners and nutrients made available from the extracellular enzymatic activities of the associated bacteria (73). Some freeliving bacteria can even spend most of their time in nutrient agglomerations in the surroundings of or within particles (74).…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Mohit

Archambault

Toupoint

et al. 2014

Appl Environ Microbiol

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bMost of what is known about coastal free-living and attached bacterial diversity is based on open coasts, with high particulate and nutrient riverine supply, terrestrial runoffs, and anthropogenic activities. The Magdalen Islands in the Gulf of St. Lawrence (Canada) are dominated by shallow lagoons with small, relatively pristine catchments and no freshwater input apart from rain. Such conditions provided an opportunity to investigate coastal free-living and attached marine bacterial diversity in the absence of confounding effects of steep freshwater gradients. We found significant differences between the two communities and marked temporal patterns in both. Taxonomic richness and diversity were greater in the attached than in the free-living community, increasing over summer, especially within the least abundant bacterial phyla. The highest number of reads fell within the SAR 11 clade (Pelagibacter, Alphaproteobacteria), which dominated free-living communities. The attached communities had deeper phylum-level diversity than the free-living fraction. Distance-based redundancy analysis indicated that the particulate organic matter (POM) concentration was the main variable separating early and late summer samples with salinity and temperature changes also significantly correlated to bacterial community structure. Our approach using high-throughput sequencing detected differences in free-living versus attached bacteria in the absence of riverine input, in keeping with the concept that marine attached communities are distinct from cooccurring free-living taxa. This diversity likely reflects the diverse microhabitats of available particles, implying that the total bacterial diversity in coastal systems is linked to particle supply and variability, with implications for understanding microbial biodiversity in marine systems.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Mohit

Archambault

Toupoint

et al. 2014

Appl Environ Microbiol

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“…The C16:1cis, C16: 1trans, C16:0, C17:1, C18:1cis, and C18:1trans profiles and the C16:1/C18:1 and C16:1cis/C16:1trans ratios were used to obtain the grouping of the most similar samples according to the structural (DRgamma = C16:1/C18:1 ? Brtot; C17:1; Bertone et al, 1996;Blažina et al, 2005Blažina et al, , 2009) as well as physiological (C16:1cis, C16:1trans; C18:1cis, C18:1trans, cis/trans) similarities of the bacterial communities.…”

Section: Discussionmentioning

confidence: 97%

“…We selected samples characterised as freshly formed aggregates from all of the data processed in previous studies (Najdek et al, 2002Blažina et al, 2005;Blažina et al, 2009). Fatty acid profiles of cultured bacterial communities from fresh mucilages (with C. closterium dominance), their adjacent waters, oligotrophic high-salinity waters and halocline waters were investigated and compared after incubation under identical conditions in culture media.…”

Section: Introductionmentioning

confidence: 99%

Microbial communities in northern Adriatic mucilaginous aggregates: insight into the early phase of aggregate formation

et al. 2010

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Insight into the initial phase of aggregate formation was provided by comparison between bacterial communities from freshly formed aggregates dominated by the epipelic diatom Cylindrotheca closterium and associated water masses. This study was performed from 2000 to 2006 in the northern Adriatic. The chemotaxonomic structures and physiological conditions were inferred from the fatty acid profiles of the cultured bacterial communities of all implicated components, fresh aggregates, their adjacent waters, oligotrophic high-salinity waters and halocline waters. The results showed similarity between bacterial communities of fresh aggregates and oligotrophic highsalinity water, suggesting their common origin and involvement in the formation of aggregates. In contrast, the origin of the water adjacent to aggregates was different from that of the other components but was similar to the halocline layer and was likely derived from northern Adriatic waters. The presence and activity of heterotrophic bacteria belonging to Alteromonadaceae, which are regularly observed on fresh aggregates, suggest that the early phase of aggregate formation corresponds to an abrupt change of environmental conditions due to the mixing of central and northern Adriatic waters. The initial colonisation of fresh mucilage ascribes to Alteromonas an important ecological function in aggregate community development related to the succession of phytoplankton and heterotrophic bacteria.

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“…This method allows consideration of probability to derive statistical inferences about the groups of observations. It has been widely applied to scientific studies, and recent ones include in manufacturing material, medical, chemical, oceanographic and food [21][22][23][24][25][26].…”

Section: Related Literaturementioning

confidence: 99%

Fault Diagnosis of Shaft- Ball Bearing System Using One-Way Analysis of Variance

Yüzükırmızı

Arslan

2014

MCA

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Abstract-Roller bearing is one of the most widely used and critical elements in rotating machinery. In consequence, bearing fault diagnosis in machines, as well as to discriminate the different fault conditions have been a great interest. In this study, firstly, analytical model of a shaft-ball bearing system is developed. The shaft is assumed to be perfectly rigid and uniform, and supported by two radial ball bearings. Then, the effect of localized defects on bearing running surfaces (i.e. surfaces of inner and outer rings and balls) on the shaft vibrations are obtained using the simulation program. Then, vibration signatures are analyzed by one-way analysis of variance (ANOVA) method. Finally, post-hoc tests are applied to differentiate the ball bearing element's localized defects in shaft-ball bearing simulation model.

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Characterisation and differentiation of oligotrophic waters by culturable particle-attached and free-living bacterial communities

Cited by 7 publications

References 18 publications

Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Phylogenetic Differences in Attached and Free-Living Bacterial Communities in a Temperate Coastal Lagoon during Summer, Revealed via High-Throughput 16S rRNA Gene Sequencing

Microbial communities in northern Adriatic mucilaginous aggregates: insight into the early phase of aggregate formation

Fault Diagnosis of Shaft- Ball Bearing System Using One-Way Analysis of Variance

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