Characterisation and evaluation of the efficiency of petroleum degrading bacteria isolated from soils around the oil exploration areas in western Uganda

Wedulo, Andrew; Atuhaire, David Kalenzi; Ochwo, Sylvester; Muwanika, Vincent B.; Rwendeire, Abel John Julian; Nakavuma, Jesca

doi:10.5897/ajb2014.13888

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…The results presented are the averages and standard deviations for triplicate assays. Wedulo et al, 2014;Lumactud et al, 2016), although other strains within these genera do not have this ability, even when detected/isolated from petroleum-polluted sites (Rajaei et al, 2013;Subhash and Lee, 2018). In fact, the presence of bacterial strains that are not able to degrade hydrocarbons in oilcontaminated environments is quite common, generally ascribed to their tolerance to hydrocarbons or to their involvement in the degradation of intermediary compounds.…”

Section: Effect Of Corksorb On Growth and Alkane Degradation By Hydrocarbonoclastic Bacteriamentioning

confidence: 99%

Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria

et al. 2021

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Biosorbent materials are effective in the removal of spilled oil from water, but their effect on hydrocarbonoclastic bacteria is not known. Here, we show that corksorb, a cork-based biosorbent, enhances growth and alkane degradation by Rhodococcus opacus B4 (Ro) and Alcanivorax borkumensis SK2 (Ab). Ro and Ab degraded 96 ± 1% and 72 ± 2%, respectively, of a mixture of n-alkanes (2 g L–1) in the presence of corksorb. These values represent an increase of 6 and 24%, respectively, relative to the assays without corksorb. The biosorbent also increased the growth of Ab by 51%. However, no significant changes were detected in the expression of genes involved in alkane uptake and degradation in the presence of corksorb relative to the control without the biosorbent. Nevertheless, transcriptomics analysis revealed an increased expression of rRNA and tRNA coding genes, which confirms the higher metabolic activity of Ab in the presence of corksorb. The effect of corksorb is not related to the release of soluble stimulating compounds, but rather to the presence of the biosorbent, which was shown to be essential. Indeed, scanning electron microscopy images and downregulation of pili formation coding genes, which are involved in cell mobility, suggest that cell attachment on corksorb is a determinant for the improved activity. Furthermore, the existence of native alkane-degrading bacteria in corksorb was revealed, which may assist in situ bioremediation. Hence, the use of corksorb in marine oil spills may induce a combined effect of sorption and stimulated biodegradation, with high potential for enhancing in situ bioremediation processes.

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Section: Effect Of Corksorb On Growth and Alkane Degradation By Hydrocarbonoclastic Bacteriamentioning

confidence: 99%

Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria

et al. 2021

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Section: Effect Of Corksorb On Growth and Alkane Degradation By Hydrocarbonoclastic Bacteriamentioning

confidence: 99%

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Diesel Biodegradation Capacities and Biosurfactant Production in Saline-Alkaline Conditions by Delftia sp NL1, Isolated from an Algerian Oilfield

Lenchi

Kebbouche-Gana

Servais

et al. 2020

Geomicrobiology Journal

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In this study, a diesel oil-degrading bacterium was isolated from an oilfield water injection (waterbearing formations, 1,205 m depth) in Algeria. The bacterial strain, designated NL1, was cultivated on diesel oil as sole carbon and energy sources. Molecular analyses of the 16S rRNA gene sequence (KY397882) placed NL1 strain closely related to distinct cultivated species of the Delftia genus. Optimal diesel oil biodegradation by Delftia sp NL1 strain occurred at pH 11, 40 C, 2 M NaCl and initial hydrocarbon concentration of 5% (v/v) as sole carbon source. GC-MS analyses evidenced that strain Delftia sp NL1 was able to degrade more than 66.76% of diesel oil within only 7 days. On the other hand, and in the same conditions, biosurfactant production by Delftia sp NL1 was also evaluated evidencing high emulsifying capacity (E 24 ¼ 81%), ability to lower the surface tension of growing media (with the value of 25.7 mN m 21 ), and production of glycolipids (8.7 g L À1 ) as biosurfactants. This research presents indigenous strain Delftia sp NL1 for diesel degradation and synthesis of biosurfactant in extreme conditions. In this sense, strain NL1 is a good candidate for possible in situ oil recovery and in wastewater treatment in refineries and oil terminals in petroleum industry.

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Characterisation and evaluation of the efficiency of petroleum degrading bacteria isolated from soils around the oil exploration areas in western Uganda

Cited by 4 publications

References 20 publications

Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria

Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria

Data_Sheet_1.pdf

Diesel Biodegradation Capacities and Biosurfactant Production in Saline-Alkaline Conditions by Delftia sp NL1, Isolated from an Algerian Oilfield

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