Characterisation and in vitro evaluation of bioadhesive liposome gels for local therapy of vaginitis

Pavelić, Željka; Škalko‐Basnet, Nataša; Jalšenjak, Ivan

doi:10.1016/j.ijpharm.2005.05.022

Cited by 89 publications

(42 citation statements)

References 21 publications

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“…The release of the drug from the liposomes incorporated into different vehicles was determined by the method originally introduced by Pesckha et al 25 and adapted for liposomal hydrogels 14,16,23 . Briefly, samples of the liposomes-in-vehicle formulations (3 g) were placed in glass vials and separated from 5 mL of receptor solution (distilled water) by a 2% (w/w) agarose layer, which permitted diffusion of both the released drug and intact liposomes from liposomes-in-vehicles (donor compartment).…”

Section: 5mentioning

confidence: 99%

“…To follow the release of diclofenac sodium from the liposomes-in-vehicle formulations, we applied a so-called agarose method that has been shown to be very useful in evaluating drug release from liposomal vaginal gels 14,16,23 . The porosity of the agarose matrix permitted both intact liposomes and released (free) drug to diffuse through the matrix into the receptor 10 solution, and the amount of drug released from the vehicle was determined spectrophotometrically before and after the addition of methanol to the supernatant over the agarose matrix 16 .…”

Section: Liposomes-in-vehicle Formulationsmentioning

confidence: 99%

“…The porosity of the agarose matrix permitted both intact liposomes and released (free) drug to diffuse through the matrix into the receptor solution, and the amount of drug released from the vehicle was determined spectrophotometrically before and after the addition of methanol to the supernatant over the agarose matrix 16 . With this method, the amount of intact liposomes released from the vehicle into the receptor medium can be determined, which is not feasible using the Franz diffusion cell method.…”

Section: Liposomes-in-vehicle Formulationsmentioning

confidence: 99%

“…Thus, they must be incorporated into a suitable vehicle 14 . In addition to increasing the viscosity and preserving physical stability of conventional liposomes incorporated into hydrogels [15][16][17][18] , dermatological vehicles could affect drug bioavailability and play an important role in the care and maintenance of skin health 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Vehicle composition has been shown to influence the release properties of the incorporated drug and the penetration ability across/into the skin and may also improve the skin condition 3,4,33 . To examine the effect of the vehicle on the release of liposomally entrapped diclofenac sodium, different types of liposomes were incorporated into the following vehicles: the hydrogel, the cream base (O/W emulsion) and DMS base.To follow the release of diclofenac sodium from the liposomes-in-vehicle formulations, we applied a so-called agarose method that has been shown to be very useful in evaluating drug release from liposomal vaginal gels 14,16,23 . The porosity of the agarose matrix permitted both intact liposomes and released (free) drug to diffuse through the matrix into the receptor 10 solution, and the amount of drug released from the vehicle was determined spectrophotometrically before and after the addition of methanol to the supernatant over the agarose matrix 16 .…”

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confidence: 99%

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Elastic liposomes-in-vehicle formulations destined for skin therapy: the synergy between type of liposomes and vehicle

Palac

Hurler

Škalko‐Basnet

et al. 2014

Drug Development and Industrial Pharmacy

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Objective: The present study is focused on optimization of elastic liposomes-in-vehicle formulations in respect to drug release and formulation properties. By combining penetration potential of elastic liposomes containing high ratio of entrapped drug and physicochemical properties of vehicles, both affecting the release and texture properties, optimal formulation could be achieved. Materials and methods:Deformable, propylene glycol-containing or conventional liposomes with hydrophilic model drug (diclofenac sodium) were incorporated into the following vehicles appropriate for skin application: a hydrogel, a cream base and derma membrane structure base cream (DMS base). Each formulation was assessed for in vitro drug release and mechanical properties. Results and discussion:The composition and type of both liposomes and the vehicle affected the rate and amount of the released drug. The cream base exhibited the slowest release, followed by the hydrogel and DMS base. Similar release profiles were achieved with both types of elastic vesicles (deformable and propylene glycol liposomes); the slowest release was observed for conventional liposomes, regardless of the vehicle used. The drug release profiles from different liposomes-in-vehicle formulations were in agreement with the physicochemical properties of the formulations. All of the liposomes were found to be compatible with the hydrogel preserving its original textures, whereas a significant decrease in all texture parameters was observed for liposomes-in-DMS base, regardless of liposome type. Conclusion:Propylene glycol liposomes-in-hydrogel is considered as the optimal formulation for improving skin delivery of hydrophilic drug. Further investigations involving in vivo animal studies are necessary to confirm its applicability in skin therapy. INTRODUCTIONEffective (trans)dermal drug delivery depends on both the selection of an appropriate drug and the properties of the delivery system (vehicle). Vehicles exert pronounced effects on the epidermis, affecting hydration, lubrication, drying, skin smoothness, occlusion and protection.In addition, the physicochemical properties of vehicles influence the pharmacokinetic properties of the incorporated drug by controlling its release from the vehicle and affecting penetration through the stratum corneum, permeation through the layers of skin and absorption into the blood. Preparation and characterization of liposomesDeformable, propylene glycol and conventional liposomes were prepared by the film 6The mean diameters, size distributions (polydispersity) and zeta potentials of the liposomes were determined by photon correlation spectroscopy (Zetasizer 3000HS, MalvernInstruments, Malvern, UK) 20,21 . Membrane elasticity of extruded liposomes was evaluated by home-made device at an external pressure of 5 bar using 100-nm membranes. Detailed procedure with equation for calculation of the deformability degree (E) has been previously described in details 14,19 . Separation of the unentrapped drug was performed usin...

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Section: 5mentioning

confidence: 99%

Section: Liposomes-in-vehicle Formulationsmentioning

confidence: 99%

Section: Liposomes-in-vehicle Formulationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Elastic liposomes-in-vehicle formulations destined for skin therapy: the synergy between type of liposomes and vehicle

Palac

Hurler

Škalko‐Basnet

et al. 2014

Drug Development and Industrial Pharmacy

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Liposomes and Drug Delivery

Antimisiaris

Kallinteri

Fatouros³

2010

Pharmaceutical Sciences Encyclopedia

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Liposomes are vesicles in which an aqueous volume is entirely surrounded by a phospholipid membrane and their size can range between 30 and 50 nm up to several micrometers. Liposomes serve as a drug delivery system because of their versatile structure and because they are nontoxic, nonimmunogenic, and fully biodegradable. This chapter focuses on basic characteristics and applications of liposomes, their fate after in vivo administration by different routes, and other possible routes for in vivo administration of liposomes.

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Vaginal Drug Delivery

Neves

Amaral

Bahia

2010

Pharmaceutical Sciences Encyclopedia

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The vagina has been used for a long time as a route for drug administration. Vaginal drug delivery has seen recent advances that make it very promising, in particular therapeutic fields such as the prevention of human immunodeficiency virus (HIV) and other sexually transmitted infections, and contraception. This article discusses the main features of vaginal anatomy, physiology, and histology related to drug delivery as well as vaginal drug delivery systems and their evaluation, current usage of the vagina as a route of drug administration, and promising strategies in this field.

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Characterisation and in vitro evaluation of bioadhesive liposome gels for local therapy of vaginitis

Cited by 89 publications

References 21 publications

Elastic liposomes-in-vehicle formulations destined for skin therapy: the synergy between type of liposomes and vehicle

Elastic liposomes-in-vehicle formulations destined for skin therapy: the synergy between type of liposomes and vehicle

Liposomes and Drug Delivery

Vaginal Drug Delivery

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